Restriction map for
pFN21AB8236
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 827 TCCGGA T^CCGGA
AciI 423 GCGG CCGC(-3/-1)
AcsI 799 AAATTC [GA]^AATT[CT]
AfaI 247 GTAC GT^AC
AocI 33 CCTGAGG CC^T[ACGT]AGG
ApoI 799 AAATTC [GA]^AATT[CT]
Asp700I 819 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 115 GAGCAC G[AT]GC[AT]^C
AvaII 718 GGACC G^G[AT]CC
BanII 27 GAGCCC G[GA]GC[CT]^C
BbsI 733 GAAGAC GAAGAC(2/6)
BbvI 475 GCTGC GCAGC(8/12)
BfaI 722 CTAG C^TAG
BglII 855 AGATCT A^GATCT
BpuAI 733 GAAGAC GAAGAC(2/6)
BsaWI 827 TCCGGA [AT]^CCGG[AT]
BseAI 827 TCCGGA T^CCGGA
BsiHKAI 115 GAGCAC G[AT]GC[AT]^C
BsiYI 433 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 433 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 876 GTCTC GTCTC(1/5)
Bsp1407I 246 TGTACA T^GTACA
BspEI 827 TCCGGA T^CCGGA
BsrDI 190 GCAATG GCAATG(2/0)
BsrGI 246 TGTACA T^GTACA
BssKI 433 CCTGG ^CC[ACGT]GG
BstNI 433 CCTGG CC^[AT]GG
BstYI 855 AGATCT [GA]^GATC[CT]
Bsu36I 33 CCTGAGG CC^T[ACGT]AGG
Cac8I 753 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 568 GCGC GCG^C
Eco81I 33 CCTGAGG CC^T[ACGT]AGG
EcoRII 433 CCTGG ^CC[AT]GG
EcoT14I 264 CCTTGG C^C[AT][AT]GG
Fnu4HI 475 GCTGC GC^[ACGT]GC
HaeII 567 AGCGCC [GA]GCGC^[CT]
HapII 828 CCGG C^CGG
HhaI 568 GCGC GCG^C
HinP1I 568 GCGC G^CGC
HpaII 828 CCGG C^CGG
ItaI 475 GCTGC GC^[ACGT]GC
MaeI 722 CTAG C^TAG
MaeIII 220 GTAAC ^GT[ACGT]AC
MflI 855 AGATCT [GA]^GATC[CT]
MroI 827 TCCGGA T^CCGGA
MspI 828 CCGG C^CGG
MvaI 433 CCTGG CC^[AT]GG
MwoI 601 GCCAAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PpuMI 717 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 247 GTAC GT^AC
ScrFI 433 CCTGG CC^[ACGT]GG
SfaNI 364 GATGC GCATC(5/9)
SfcI 728 CTGTAG C^T[GA][CT]AG
SspBI 246 TGTACA T^GTACA
StyI 264 CCTTGG C^C[AT][AT]GG
TaqI 10 TCGA T^CGA
TseI 475 GCTGC G^C[AT]GC
TthHB8I 10 TCGA T^CGA
XhoII 855 AGATCT [GA]^GATC[CT]
XmnI 819 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 297 CTTAAG C^TTAAG
405 CTTAAG
AluI 351 AGCT AG^CT
555 AGCT
BfrI 297 CTTAAG C^TTAAG
405 CTTAAG
BmyI 27 GAGCCC G[AGT]GC[ACT]^C
115 GAGCAC
BpmI 596 CTCCAG CTGGAG(16/14)
434 CTGGAG
BsaJI 264 CCTTGG C^C[ACGT][ACGT]GG
432 CCCTGG
BseRI 385 GAGGAG GAGGAG(10/8)
388 GAGGAG
Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C
115 GAGCAC
DdeI 34 CTGAG C^T[ACGT]AG
345 CTTAG
DpnII 662 GATC ^GATC
856 GATC
DraII 37 AGGCCCC [GA]G^G[ACGT]CC[CT]
717 AGGACCT
EcoO109I 37 AGGCCCC [GA]G^G[ACGT]CC[CT]
717 AGGACCT
MboI 662 GATC ^GATC
856 GATC
MslI 18 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
328 CATATTAGTG
NdeII 662 GATC ^GATC
856 GATC
NlaIII 18 CATG CATG^
24 CATG
NlaIV 38 GGCCCC GG[ACGT]^[ACGT]CC
450 GGTTCC
PleI 637 GACTC GAGTC(4/5)
370 GAGTC
Sau3AI 662 GATC ^GATC
856 GATC
SmlI 297 CTTAAG C^T[CT][GA]AG
405 CTTAAG
TfiI 7 GATTC G^A[AT]TC
676 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BsrI BstF5I Cfr13I EarI FokI
HaeIII HinfI Ksp632I MboII MnlI
MseI Sau96I Tru9I Tsp509I TspRI