(Circular) (Six-base) MAPSORT of: pFN21AB8235.seq Check: 382 from: 1 to: 5365 pFN21AB8235 5365 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2626 2626 Size: 5365 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2114 3175 278 Size: 53 83 186 936 578 1061 2468 Fragments arranged by size: 2468 1061 936 578 186 83 53 AccI GT'mk_AC Cuts at: 1937 2708 1937 Size: 771 4594 Acc65I G'GTAC_C Cuts at: 2253 2686 2253 Size: 433 4932 AclI AA'CG_TT Cuts at: 1563 3492 3865 1563 Size: 1929 373 3063 Fragments arranged by size: 3063 1929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3428 4812 5080 1632 Size: 1796 1384 268 1917 Fragments arranged by size: 1917 1796 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5168 AflIII A'CryG_T Cuts at: 1203 2067 4264 5241 1203 Size: 864 2197 977 1327 Fragments arranged by size: 2197 1327 977 864 AgeI A'CCGG_T Cuts at: 4196 4196 Size: 5365 AhdI GACnn_n'nnGTC Cuts at: 4094 4094 Size: 5365 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5365 AlwNI CAG_nnn'CTG Cuts at: 2395 3108 4680 2395 Size: 713 1572 3080 Fragments arranged by size: 3080 1572 713 ApaI G_GGCC'C Cuts at: 2618 2618 Size: 5365 ApaLI G'TGCA_C Cuts at: 3422 4578 3422 Size: 1156 4209 ApoI r'AATT_y Cuts at: 1493 2674 2900 3052 5161 1493 Size: 1181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1181 226 152 AseI AT'TA_AT Cuts at: 160 1032 3919 160 Size: 872 2887 1606 Fragments arranged by size: 2887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 AvaI C'yCGr_G Cuts at: 1870 1943 2690 1870 Size: 73 747 4545 Fragments arranged by size: 4545 747 73 BamHI G'GATC_C Cuts at: 2695 3062 2695 Size: 367 4998 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 2603 2638 2686 4141 Size: 325 217 627 466 350 35 48 1455 Cuts at: 4141 618 Size: 1842 Fragments arranged by size: 1842 1455 627 466 350 325 217 48 35 BanII G_rGCy'C Cuts at: 1391 2618 2684 1391 Size: 1227 66 4072 Fragments arranged by size: 4072 1227 66 BbeI G_GCGC'C Cuts at: 2607 2607 Size: 5365 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3556 3590 1903 Size: 34 1619 34 3678 Fragments arranged by size: 3678 1619 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3257 4467 3257 Size: 1210 4155 BclI T'GATC_A Cuts at: 1548 1548 Size: 5365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3976 136 Size: 107 122 71 631 605 2304 1525 Fragments arranged by size: 2304 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2226 5360 2226 Size: 3134 2231 BlpI GC'TnA_GC Cuts at: 2782 2782 Size: 5365 Bme1580I G_kGCm'C Cuts at: 1790 2618 3426 4582 1790 Size: 828 808 1156 2573 Fragments arranged by size: 2573 1156 828 808 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3096 4054 442 Size: 416 53 83 399 24 1679 958 1753 Fragments arranged by size: 1753 1679 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2465 1056 Size: 1409 3956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4025 1239 Size: 2786 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3490 4355 4653 3490 Size: 865 298 4202 Fragments arranged by size: 4202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4028 882 Size: 617 2529 2219 Fragments arranged by size: 2529 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3156 493 Size: 711 1952 2702 Fragments arranged by size: 2702 1952 711 BsaBI GATnn'nnATC Cuts at: 2081 5359 2081 Size: 3278 2087 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 2604 3172 3554 Size: 53 83 186 936 578 493 568 382 Cuts at: 3554 4189 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 578 568 493 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2256 3798 4196 4470 4617 5028 2256 Size: 1542 398 274 147 411 2593 Fragments arranged by size: 2593 1542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5140 Fragments arranged by size: 5140 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5365 BseYI C'CCAG_C Cuts at: 4568 5064 4568 Size: 496 4869 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 1163 Size: 573 627 4165 Fragments arranged by size: 4165 627 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2815 3576 4604 1540 Size: 275 186 814 761 1028 2301 Fragments arranged by size: 2301 1028 814 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3426 3511 4582 3426 Size: 85 1071 4209 Fragments arranged by size: 4209 1071 85 BsmI GAATG_Cn' Cuts at: 2876 2969 2876 Size: 93 5272 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2618 2684 3426 3511 4582 1391 Size: 399 828 66 742 85 1071 2174 Fragments arranged by size: 2174 1071 828 742 399 85 66 BspEI T'CCGG_A Cuts at: 5028 5028 Size: 5365 BspHI T'CATG_A Cuts at: 3254 4984 3254 Size: 1730 3635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2626 2720 844 Size: 1782 94 3489 Fragments arranged by size: 3489 1782 94 BsrBI CCG'CTC Cuts at: 1394 1823 2251 2431 3252 1394 Size: 429 428 180 821 3507 Fragments arranged by size: 3507 821 429 428 180 BsrDI GCAATG_nn' Cuts at: 65 1248 3860 4034 65 Size: 1183 2612 174 1396 Fragments arranged by size: 2612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2454 4009 4196 1700 Size: 110 644 1555 187 2869 Fragments arranged by size: 2869 1555 644 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5365 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5365 BssSI C'ACGA_G Cuts at: 3425 4437 3425 Size: 1012 4353 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5365 BstBI TT'CG_AA Cuts at: 4169 4169 Size: 5365 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5365 BstYI r'GATC_y Cuts at: 1972 2226 2695 3062 3447 3464 4916 5002 5014 Size: 254 469 367 385 17 1452 86 12 Cuts at: 5014 5360 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 469 385 367 346 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 3712 Fragments arranged by size: 3712 939 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4626 Fragments arranged by size: 4626 683 56 BtsI GCAGTG_nn' Cuts at: 2346 2881 3675 3695 2346 Size: 535 794 20 4016 Fragments arranged by size: 4016 794 535 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5330 DraI TTT'AAA Cuts at: 2669 2669 Size: 5365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2183 2594 4372 809 Size: 507 867 411 1778 1802 Fragments arranged by size: 1802 1778 867 507 411 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2452 2812 3701 Size: 54 1425 50 275 18 622 360 889 Cuts at: 3701 5330 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 622 360 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2812 1537 Size: 275 1000 4090 Fragments arranged by size: 4090 1000 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 3295 1353 Size: 773 1169 3423 Fragments arranged by size: 3423 1169 773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2002 2600 3952 4326 4472 387 Size: 1064 138 413 598 1352 374 146 1280 Fragments arranged by size: 1352 1280 1064 598 413 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3428 4025 4812 5080 1239 Size: 393 1796 597 787 268 1524 Fragments arranged by size: 1796 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2614 2614 Size: 5365 EcoRI G'AATT_C Cuts at: 2674 3052 2674 Size: 378 4987 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2131 2545 2131 Size: 414 4951 FspI TGC'GCA Cuts at: 3871 3871 Size: 5365 HaeII r_GCGC'y Cuts at: 1678 2289 2502 2539 2607 4512 1678 Size: 611 213 37 68 1905 2531 Fragments arranged by size: 2531 1905 611 213 68 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2182 2214 2549 2581 4006 Size: 32 135 32 610 32 335 32 1425 Cuts at: 4006 4038 4080 4112 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1425 610 335 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2709 2955 669 Size: 988 281 771 246 3079 Fragments arranged by size: 3079 988 771 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5365 HpaI GTT'AAC Cuts at: 2955 2955 Size: 5365 KasI G'GCGC_C Cuts at: 2603 2603 Size: 5365 KpnI G_GTAC'C Cuts at: 2257 2690 2257 Size: 433 4932 MfeI C'AATT_G Cuts at: 2964 2964 Size: 5365 MluI A'CGCG_T Cuts at: 5241 5241 Size: 5365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 4479 4663 1305 Size: 1251 1923 184 2007 Fragments arranged by size: 2007 1923 1251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3543 Fragments arranged by size: 3543 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 3323 3682 3841 518 Size: 648 1137 1020 359 159 2042 Fragments arranged by size: 2042 1137 1020 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2781 3458 4606 4851 1606 Size: 1175 677 1148 245 2120 Fragments arranged by size: 2120 1175 1148 677 245 NaeI GCC'GGC Cuts at: 2456 2456 Size: 5365 NarI GG'CG_CC Cuts at: 2604 2604 Size: 5365 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3874 Fragments arranged by size: 3874 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5365 NgoMIV G'CCGG_C Cuts at: 2454 2454 Size: 5365 NheI G'CTAG_C Cuts at: 1052 2461 1052 Size: 1409 3956 NotI GC'GGCC_GC Cuts at: 2812 2812 Size: 5365 NspI r_CATG'y Cuts at: 1132 1229 2071 2723 4268 1132 Size: 97 842 652 1545 2229 Fragments arranged by size: 2229 1545 842 652 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5365 PciI A'CATG_T Cuts at: 2067 4264 2067 Size: 2197 3168 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5365 PmeI GTTT'AAAC Cuts at: 2669 2669 Size: 5365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4947 4979 3434 Size: 32 1481 32 3820 Fragments arranged by size: 3820 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5365 PsiI TTA'TAA Cuts at: 2935 2935 Size: 5365 PspOMI G'GGCC_C Cuts at: 2614 2614 Size: 5365 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2048 2080 2048 Size: 32 5333 PstI C_TGCA'G Cuts at: 830 2226 2717 830 Size: 1396 491 3478 Fragments arranged by size: 3478 1396 491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5365 SalI G'TCGA_C Cuts at: 1936 2707 1936 Size: 771 4594 SbfI CC_TGCA'GG Cuts at: 2717 2717 Size: 5365 ScaI AGT'ACT Cuts at: 3613 5325 3613 Size: 1712 3653 SfcI C'TryA_G Cuts at: 826 1045 2222 2713 3848 4529 4720 826 Size: 219 1177 491 1135 681 191 1471 Fragments arranged by size: 1471 1177 1135 681 491 219 191 SfoI GGC'GCC Cuts at: 2605 2605 Size: 5365 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5365 SmaI CCC'GGG Cuts at: 1872 2692 1872 Size: 820 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3469 4370 4632 820 Size: 197 926 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3156 493 Size: 2663 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5365 SphI G_CATG'C Cuts at: 1132 2723 1132 Size: 1591 3774 SspI AAT'ATT Cuts at: 5 52 3289 5 Size: 47 3237 2081 Fragments arranged by size: 3237 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5365 StyI C'CwwG_G Cuts at: 513 1065 2004 2473 2502 2804 513 Size: 552 939 469 29 302 3074 Fragments arranged by size: 3074 939 552 469 302 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2372 3392 3409 3562 3747 4192 325 Size: 1255 792 1020 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1020 792 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3611 5323 96 Size: 274 80 33 51 1443 1634 1712 138 Fragments arranged by size: 1712 1634 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5325 5357 5325 Size: 32 5333 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5365 XbaI T'CTAG_A Cuts at: 2701 2701 Size: 5365 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5365 XmaI C'CCGG_G Cuts at: 1870 2690 1870 Size: 820 4545 XmnI GAAnn'nnTTC Cuts at: 3494 3494 Size: 5365 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2112 3173 276 Size: 53 83 186 936 578 1061 2468 Fragments arranged by size: 2468 1061 936 578 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI