Restriction map for
pFN21AB8231
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI AspEI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
Ksp632I KspI MaeII MaeIII MluI
MluNI MscI MseI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tsp45I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 335 GGTACC G^GTACC
AccIII 453 TCCGGA T^CCGGA
AciI 534 GCGG CCGC(-3/-1)
AfaI 336 GTAC GT^AC
AocI 310 CCTAAGG CC^T[ACGT]AGG
ApaI 207 GGGCCC GGGCC^C
Asp700I 238 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 335 GGTACC G^GTACC
AspHI 230 GAGCTC G[AT]GC[AT]^C
AspI 97 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 167 CCCGAG C^[CT]CG[GA]G
BamHI 624 GGATCC G^GATCC
BanI 335 GGTACC G^G[CT][GA]CC
BbvI 126 GCTGC GCAGC(8/12)
BfaI 388 CTAG C^TAG
BsaBI 619 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 85 CCCCGG C^C[ACGT][ACGT]GG
BsaWI 453 TCCGGA [AT]^CCGG[AT]
BseAI 453 TCCGGA T^CCGGA
BsiHKAI 230 GAGCTC G[AT]GC[AT]^C
BsmBI 525 GAGACG CGTCTC(1/5)
BsmFI 598 GTCCC GGGAC(10/14)
BsoBI 167 CCCGAG C^[CT]CG[GA]G
BspEI 453 TCCGGA T^CCGGA
BspHI 234 TCATGA T^CATGA
BsrDI 156 CATTGC GCAATG(2/0)
BstXI 275 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 624 GGATCC [GA]^GATC[CT]
Bsu36I 310 CCTAAGG CC^T[ACGT]AGG
Cac8I 48 GCTGGC GC[ACGT]^[ACGT]GC
Eco81I 310 CCTAAGG CC^T[ACGT]AGG
EcoRV 115 GATATC GAT^ATC
Fnu4HI 126 GCTGC GC^[ACGT]GC
HincII 291 GTTGAC GT[CT]^[GA]AC
HindII 291 GTTGAC GT[CT]^[GA]AC
HphI 186 TCACC GGTGA(8/7)
ItaI 126 GCTGC GC^[ACGT]GC
KpnI 335 GGTACC GGTAC^C
MaeI 388 CTAG C^TAG
MamI 619 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 507 GAAGA GAAGA(8/7)
MfeI 148 CAATTG C^AATTG
MflI 624 GGATCC [GA]^GATC[CT]
MroI 453 TCCGGA T^CCGGA
MslI 53 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 148 CAATTG C^AATTG
PflFI 97 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 63 GACTC GAGTC(4/5)
PpuMI 255 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 407 CTGCAG CTGCA^G
RcaI 234 TCATGA T^CATGA
RsaI 336 GTAC GT^AC
SacI 230 GAGCTC GAGCT^C
SfcI 407 CTGCAG C^T[GA][CT]AG
TaqI 420 TCGA T^CGA
TfiI 198 GAATC G^A[AT]TC
TseI 126 GCTGC G^C[AT]GC
Tsp509I 149 AATT ^AATT
TspRI 405 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 97 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 420 TCGA T^CGA
XbaI 387 TCTAGA T^CTAGA
XhoII 624 GGATCC [GA]^GATC[CT]
XmnI 238 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AluI 183 AGCT AG^CT
231 AGCT
AlwNI 121 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
565 CAGAGACTG
AvaII 89 GGACC G^G[AT]CC
256 GGTCC
BanII 207 GGGCCC G[GA]GC[CT]^C
230 GAGCTC
BcnI 86 CCCGG CC^[GC]GG
99 CCGGG
BmyI 207 GGGCCC G[AGT]GC[ACT]^C
230 GAGCTC
BseRI 518 GAGGAG GAGGAG(10/8)
537 GAGGAG
Bsp1286I 207 GGGCCC G[AGT]GC[ACT]^C
230 GAGCTC
DdeI 105 CTCAG C^T[ACGT]AG
311 CTAAG
HinfI 63 GACTC G^A[ACGT]TC
198 GAATC
MwoI 184 GCTCACCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
209 GCCCCCAGAGC
NciI 86 CCCGG CC^[GC]GG
99 CCGGG
SfaNI 52 GCATC GCATC(5/9)
441 GCATC
XcmI 161 CCAGGCCCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
194 CCATGAATCTTGTGG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AlwI BpmI BsaI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cfr13I DpnII DraII EcoO109I EcoRII
FokI HaeIII HapII HpaII MboI
MnlI MspI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI