Restriction map for
pFN21AB8230
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbeI BbsI
BbvCI BbvI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII ItaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1009 GTCTAC GT^[AC][GT]AC
AseI 148 ATTAAT AT^TAAT
AsnI 148 ATTAAT AT^TAAT
BanI 981 GGCACC G^G[CT][GA]CC
BbrPI 236 CACGTG CAC^GTG
BcnI 632 CCCGG CC^[GC]GG
BpmI 502 CTCCAG CTGGAG(16/14)
BsaAI 236 CACGTG [CT]AC^GT[GA]
BsaI 1016 GAGACC GGTCTC(1/5)
BspDI 136 ATCGAT AT^CGAT
BssSI 656 CACGAG CACGAG(-5/-1)
BstAPI 63 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1020 CCAGCCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 72 GCGC GCG^C
ClaI 136 ATCGAT AT^CGAT
DrdI 276 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 634 CGGCCA [CT]^GGCC[GA]
HapII 633 CCGG C^CGG
HhaI 72 GCGC GCG^C
HinP1I 72 GCGC G^CGC
HincII 510 GTTAAC GT[CT]^[GA]AC
HindII 510 GTTAAC GT[CT]^[GA]AC
HindIII 416 AAGCTT A^AGCTT
HpaI 510 GTTAAC GTT^AAC
HpaII 633 CCGG C^CGG
MaeII 237 ACGT A^CGT
MaeIII 625 GTTAC ^GT[ACGT]AC
MspI 633 CCGG C^CGG
NciI 632 CCCGG CC^[GC]GG
NdeI 1025 CATATG CA^TATG
NspI 435 ACATGC [GA]CATG^[CT]
PmaCI 236 CACGTG CAC^GTG
PmlI 236 CACGTG CAC^GTG
PshBI 148 ATTAAT AT^TAAT
PstI 410 CTGCAG CTGCA^G
ScaI 356 AGTACT AGT^ACT
SmlI 969 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 772 CCGC CCGC(-3/-1)
31 GCGG
AcyI 663 GACGCC G[GA]^CG[CT]C
1006 GGCGTC
Asp700I 94 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
354 GAAGTACTTC
AvaI 19 CCCGAG C^[CT]CG[GA]G
692 CCCGAG
AvaII 48 GGACC G^G[AT]CC
232 GGTCC
BsaHI 663 GACGCC G[GA]^CG[CT]C
1006 GGCGTC
BsoBI 19 CCCGAG C^[CT]CG[GA]G
692 CCCGAG
BspMI 14 ACCTGC ACCTGC(4/8)
408 ACCTGC
Cac8I 63 GCACGC GC[ACGT]^[ACGT]GC
492 GCAAGC
DdeI 203 CTAAG C^T[ACGT]AG
922 CTCAG
DpnII 376 GATC ^GATC
486 GATC
EcoT14I 291 CCTTGG C^C[AT][AT]GG
320 CCAAGG
HgaI 663 GACGC GACGC(5/10)
1007 GCGTC
Hin1I 663 GACGCC G[GA]^CG[CT]C
1006 GGCGTC
HphI 78 GGTGA GGTGA(8/7)
144 GGTGA
MboI 376 GATC ^GATC
486 GATC
NdeII 376 GATC ^GATC
486 GATC
Sau3AI 376 GATC ^GATC
486 GATC
StyI 291 CCTTGG C^C[AT][AT]GG
320 CCAAGG
TaqI 137 TCGA T^CGA
558 TCGA
TfiI 853 GATTC G^A[AT]TC
940 GATTC
Tsp509I 183 AATT ^AATT
244 AATT
TthHB8I 137 TCGA T^CGA
558 TCGA
XmnI 94 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
354 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cfr13I EarI EcoRII
FokI HaeIII HinfI Ksp632I MboII
MnlI MseI MslI MvaI MwoI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfcI Tru9I TspRI