(Circular) (Six-base) MAPSORT of: pFN21AB8230.seq Check: 460 from: 1 to: 5743 pFN21AB8230 5743 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2422 2422 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3015 3086 1937 Size: 1078 71 4594 Fragments arranged by size: 4594 1078 71 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2976 3806 5190 5458 1632 Size: 644 700 830 1384 268 1917 Fragments arranged by size: 1917 1384 830 700 644 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 3486 5058 3486 Size: 1572 4171 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 2154 4297 160 Size: 872 1122 2143 1606 Fragments arranged by size: 2143 1606 1122 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2697 3068 1870 Size: 73 81 673 371 4545 Fragments arranged by size: 4545 673 371 81 73 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 3064 4519 618 Size: 325 217 627 1199 78 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 78 BanII G_rGCy'C Cuts at: 1391 3062 1391 Size: 1671 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4772 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4354 136 Size: 107 122 71 631 605 2682 1525 Fragments arranged by size: 2682 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 3804 4960 1790 Size: 2014 1156 2573 Fragments arranged by size: 2573 2014 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2769 3474 4432 Size: 416 53 83 399 24 1352 705 958 Cuts at: 4432 442 Size: 1753 Fragments arranged by size: 1753 1352 958 705 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2062 2744 2062 Size: 682 5061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 4403 1239 Size: 1253 1911 2579 Fragments arranged by size: 2579 1911 1253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2959 3868 4733 5031 2959 Size: 909 865 298 3671 Fragments arranged by size: 3671 909 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3015 4406 882 Size: 617 1516 1391 2219 Fragments arranged by size: 2219 1516 1391 617 BsaAI yAC'GTr Cuts at: 493 1204 2243 3534 493 Size: 711 1039 1291 2702 Fragments arranged by size: 2702 1291 1039 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2669 3012 3550 3932 Size: 53 83 186 936 1136 343 538 382 Cuts at: 3932 4567 275 Size: 635 1451 Fragments arranged by size: 1451 1136 936 635 538 382 343 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2640 2659 2670 Size: 30 165 30 597 30 610 19 11 Cuts at: 2670 2689 1178 Size: 19 4232 Fragments arranged by size: 4232 610 597 165 30 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2640 2697 2760 2948 1186 Size: 838 616 57 63 188 3981 Fragments arranged by size: 3981 838 616 188 63 57 BseYI C'CCAG_C Cuts at: 2170 2602 2884 4946 5442 2170 Size: 432 282 2062 496 2471 Fragments arranged by size: 2471 2062 496 432 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3804 3889 4960 3804 Size: 85 1071 4587 Fragments arranged by size: 4587 1071 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3062 3804 3889 4960 1391 Size: 399 1272 742 85 1071 2174 Fragments arranged by size: 2174 1272 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 2422 3098 844 Size: 1184 394 676 3489 Fragments arranged by size: 3489 1184 676 394 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 2661 3803 4815 2661 Size: 1142 1012 3589 Fragments arranged by size: 3589 1142 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2074 794 Size: 1280 4463 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 3032 3032 Size: 5743 BstYI r'GATC_y Cuts at: 1972 3073 3440 3825 3842 5294 5380 5392 5738 Size: 1101 367 385 17 1452 86 12 346 Cuts at: 5738 1972 Size: 1977 Fragments arranged by size: 1977 1452 1101 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2832 3259 4053 4073 2832 Size: 427 794 20 4502 Fragments arranged by size: 4502 794 427 20 ClaI AT'CG_AT Cuts at: 1554 2142 1554 Size: 588 5155 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2945 2980 529 Size: 35 2381 35 3292 Fragments arranged by size: 3292 2381 35 35 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2287 4750 809 Size: 507 971 2463 1802 Fragments arranged by size: 2463 1802 971 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2639 3190 4079 Size: 54 1425 50 275 18 809 551 889 Cuts at: 4079 5708 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 809 551 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2713 2955 3673 1353 Size: 975 385 242 718 3423 Fragments arranged by size: 3423 975 718 385 242 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 4330 4704 4850 387 Size: 1064 138 462 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 462 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2492 2976 3806 4403 5190 5458 Size: 393 644 216 484 830 597 787 268 Cuts at: 5458 1239 Size: 1524 Fragments arranged by size: 1524 830 787 644 597 484 393 268 216 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 5743 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 4890 1678 Size: 3212 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2659 2691 2910 2942 4384 Size: 32 135 32 1087 32 219 32 1442 Cuts at: 4384 4416 4458 4490 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1442 1087 219 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2517 3087 3333 669 Size: 988 281 579 570 246 3079 Fragments arranged by size: 3079 988 579 570 281 246 HindIII A'AGCT_T Cuts at: 748 2421 748 Size: 1673 4070 HpaI GTT'AAC Cuts at: 2517 3333 2517 Size: 816 4927 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2626 4857 5041 1305 Size: 1321 2231 184 2007 Fragments arranged by size: 2231 2007 1321 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2373 2399 3701 4060 4219 518 Size: 648 978 229 26 1302 359 159 2042 Fragments arranged by size: 2042 1302 978 648 359 229 159 26 MspA1I CmG'CkG Cuts at: 1606 3159 3836 4984 5229 1606 Size: 1553 677 1148 245 2120 Fragments arranged by size: 2120 1553 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 NdeI CA'TA_TG Cuts at: 387 3031 387 Size: 2644 3099 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2444 3101 4646 1132 Size: 97 1215 657 1545 2229 Fragments arranged by size: 2229 1545 1215 657 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5743 PfoI T'CCnGG_A Cuts at: 2508 2508 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PmlI CAC'GTG Cuts at: 2243 2243 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 2481 3313 2481 Size: 832 4911 PstI C_TGCA'G Cuts at: 830 2419 3095 830 Size: 1589 676 3478 Fragments arranged by size: 3478 1589 676 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 2363 3991 5703 2363 Size: 1628 1712 2403 Fragments arranged by size: 2403 1712 1628 SfcI C'TryA_G Cuts at: 826 1045 2415 2875 3016 3091 4226 4907 5098 Size: 219 1370 460 141 75 1135 681 191 Cuts at: 5098 826 Size: 1471 Fragments arranged by size: 1471 1370 1135 681 460 219 191 141 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2974 3847 4748 5010 820 Size: 197 926 1031 873 901 262 1553 Fragments arranged by size: 1553 1031 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2004 2296 2325 3182 513 Size: 552 939 292 29 857 3074 Fragments arranged by size: 3074 939 857 552 292 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 2361 3989 Size: 274 80 33 51 1443 159 225 1628 Cuts at: 3989 5701 96 Size: 1712 138 Fragments arranged by size: 1712 1628 1443 274 225 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2166 2198 5703 5735 2166 Size: 32 3505 32 2174 Fragments arranged by size: 3505 2174 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 2103 2363 3872 2103 Size: 260 1509 3974 Fragments arranged by size: 3974 1509 260 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI