Restriction map for
pFN21AB8230
(Linear) (Six-base) MAPSORT of: KIBB8230.seq Check: 9259 from: 1 to: 1038
KIBB8230.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 417 1038
Size: 417 621
AccI GT'mk_AC
Cuts at: 0 1010 1038
Size: 1010 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 971 1038
Size: 271 700 67
Fragments arranged by size:
700 271 67
AseI AT'TA_AT
Cuts at: 0 149 1038
Size: 149 889
AvaI C'yCGr_G
Cuts at: 0 19 692 1038
Size: 19 673 346
Fragments arranged by size:
673 346 19
BanI G'GyrC_C
Cuts at: 0 981 1038
Size: 981 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 764 1038
Size: 764 274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 57 739 1038
Size: 57 682 299
Fragments arranged by size:
682 299 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1038
Size: 487 551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 1038
Size: 954 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 1010 1038
Size: 1010 28
BsaAI yAC'GTr
Cuts at: 0 238 1038
Size: 238 800
BsaHI Gr'CG_yC
Cuts at: 0 664 1007 1038
Size: 664 343 31
Fragments arranged by size:
664 343 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 635 654 665 684 1038
Size: 25 610 19 11 19 354
Fragments arranged by size:
610 354 25 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 635 692 755 943 1038
Size: 19 616 57 63 188 95
Fragments arranged by size:
616 188 95 63 57 19
BseYI C'CCAG_C
Cuts at: 0 165 597 879 1038
Size: 165 432 282 159
Fragments arranged by size:
432 282 165 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 417 1038
Size: 23 394 621
Fragments arranged by size:
621 394 23
BssSI C'ACGA_G
Cuts at: 0 656 1038
Size: 656 382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 69 1038
Size: 69 969
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1027 1038
Size: 1027 11
BtsI GCAGTG_nn'
Cuts at: 0 827 1038
Size: 827 211
ClaI AT'CG_AT
Cuts at: 0 137 1038
Size: 137 901
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 940 975 1038
Size: 940 35 63
Fragments arranged by size:
940 63 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 282 1038
Size: 282 756
EaeI y'GGCC_r
Cuts at: 0 634 1038
Size: 634 404
EarI CTCTTCn'nnn_
Cuts at: 0 323 708 950 1038
Size: 323 385 242 88
Fragments arranged by size:
385 323 242 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 1038
Size: 46 992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 487 971 1038
Size: 271 216 484 67
Fragments arranged by size:
484 271 216 67
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 1038
Size: 228 810
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 654 686 905 937 1038
Size: 654 32 219 32 101
Fragments arranged by size:
654 219 101 32 32
HincII GTy'rAC
Cuts at: 0 512 1038
Size: 512 526
HindIII A'AGCT_T
Cuts at: 0 416 1038
Size: 416 622
HpaI GTT'AAC
Cuts at: 0 512 1038
Size: 512 526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 1038
Size: 621 417
MslI CAynn'nnrTG
Cuts at: 0 139 368 394 1038
Size: 139 229 26 644
Fragments arranged by size:
644 229 139 26
NdeI CA'TA_TG
Cuts at: 0 1026 1038
Size: 1026 12
NspI r_CATG'y
Cuts at: 0 439 1038
Size: 439 599
PfoI T'CCnGG_A
Cuts at: 0 503 1038
Size: 503 535
PmlI CAC'GTG
Cuts at: 0 238 1038
Size: 238 800
PsiI TTA'TAA
Cuts at: 0 476 1038
Size: 476 562
PstI C_TGCA'G
Cuts at: 0 414 1038
Size: 414 624
ScaI AGT'ACT
Cuts at: 0 358 1038
Size: 358 680
SfcI C'TryA_G
Cuts at: 0 410 870 1011 1038
Size: 410 460 141 27
Fragments arranged by size:
460 410 141 27
SmlI C'TyrA_G
Cuts at: 0 969 1038
Size: 969 69
StyI C'CwwG_G
Cuts at: 0 291 320 1038
Size: 291 29 718
Fragments arranged by size:
718 291 29
TatI w'GTAC_w
Cuts at: 0 131 356 1038
Size: 131 225 682
Fragments arranged by size:
682 225 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 161 193 1038
Size: 161 32 845
Fragments arranged by size:
845 161 32
XmnI GAAnn'nnTTC
Cuts at: 0 98 358 1038
Size: 98 260 680
Fragments arranged by size:
680 260 98
Enzymes that do cut:
AarI AccI AcuI AseI AvaI BanI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BspMI BssSI BstAPI BstXI BtsI ClaI CspCI DrdI
EaeI EarI EciI Eco57MI FalI Hin4I HincII HindIII
HpaI MmeI MslI NdeI NspI PfoI PmlI PsiI
PstI ScaI SfcI SmlI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI Bpu10I BsaBI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI DraI DraIII EagI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI