Restriction map for
pFN21AB8225
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 128 ACATGT A^C[GA][CT]GT
AlwI 315 GGATC GGATC(4/5)
AspHI 115 GAGCTC G[AT]GC[AT]^C
AvaI 50 CCCGAG C^[CT]CG[GA]G
BalI 143 TGGCCA TGG^CCA
BbsI 181 GAAGAC GAAGAC(2/6)
BbvCI 76 GCTGAGG CCTCAGC(-5/-2)
BpmI 415 CTGGAG CTGGAG(16/14)
BpuAI 181 GAAGAC GAAGAC(2/6)
BsiHKAI 115 GAGCTC G[AT]GC[AT]^C
BsiYI 153 CCTGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 153 CCTGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 53 GAGACG CGTCTC(1/5)
BsmFI 125 GGGAC GGGAC(10/14)
BsoBI 50 CCCGAG C^[CT]CG[GA]G
BspLU11I 128 ACATGT A^CATGT
BspMI 339 GCAGGT ACCTGC(4/8)
BstF5I 375 GGATG GGATG(2/0)
BstNI 195 CCTGG CC^[AT]GG
DsaI 65 CCGTGG C^C[GA][CT]GG
EarI 334 GAAGAG CTCTTC(1/4)
EcoRII 195 CCTGG ^CC[AT]GG
EcoT22I 377 ATGCAT ATGCA^T
FauI 46 CCCGC CCCGC(4/6)
FokI 375 GGATG GGATG(9/13)
HphI 215 TCACC GGTGA(8/7)
Ksp632I 334 GAAGAG CTCTTC(1/4)
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
MspA1I 74 CAGCTG C[AC]G^C[GT]G
MvaI 195 CCTGG CC^[AT]GG
NlaIII 129 CATG CATG^
NlaIV 90 GGAGCC GG[ACGT]^[ACGT]CC
NsiI 377 ATGCAT ATGCA^T
NspI 128 ACATGT [GA]CATG^[CT]
PstI 301 CTGCAG CTGCA^G
PvuII 74 CAGCTG CAG^CTG
SacI 115 GAGCTC GAGCT^C
SapI 334 GAAGAGC GCTCTTC(1/4)
SfcI 301 CTGCAG C^T[GA][CT]AG
SmlI 352 CTTGAG C^T[CT][GA]AG
XcmI 285 CCAGAAATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 22 GTAC GT^AC
405 GTAC
BanII 43 GAGCCC G[GA]GC[CT]^C
115 GAGCTC
BcnI 32 CCCGG CC^[GC]GG
123 CCGGG
BmyI 43 GAGCCC G[AGT]GC[ACT]^C
115 GAGCTC
BsaJI 14 CCGAGG C^C[ACGT][ACGT]GG
65 CCGTGG
BsmAI 53 GAGAC GTCTC(1/5)
70 GAGAC
Bsp1286I 43 GAGCCC G[AGT]GC[ACT]^C
115 GAGCTC
DdeI 77 CTGAG C^T[ACGT]AG
113 CTGAG
DpnII 316 GATC ^GATC
321 GATC
EaeI 143 TGGCCA [CT]^GGCC[GA]
170 CGGCCA
HaeIII 144 GGCC GG^CC
171 GGCC
HapII 33 CCGG C^CGG
123 CCGG
HpaII 33 CCGG C^CGG
123 CCGG
MboI 316 GATC ^GATC
321 GATC
MboII 181 GAAGA GAAGA(8/7)
334 GAAGA
MnlI 16 GAGG CCTC(7/6)
79 GAGG
MseI 271 TTAA T^TAA
280 TTAA
MspI 33 CCGG C^CGG
123 CCGG
NciI 32 CCCGG CC^[GC]GG
123 CCGGG
NdeII 316 GATC ^GATC
321 GATC
RsaI 22 GTAC GT^AC
405 GTAC
Sau3AI 316 GATC ^GATC
321 GATC
SfaNI 58 GATGC GCATC(5/9)
376 GATGC
Tru9I 271 TTAA T^TAA
280 TTAA
TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^
177 CAGTG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AciI AluI BbvI BsrI BssKI
Cac8I Fnu4HI ItaI MwoI ScrFI
TseI