Restriction map for
pFN21AB8225
(Circular) (Six-base) MAPSORT of: pFN21AB8225.seq Check: 2433 from: 1 to: 5131
pFN21AB8225 5131 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2941 278
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 1937
Size: 537 4594
Acc65I G'GTAC_C
Cuts at: 2452 2452
Size: 5131
AclI AA'CG_TT
Cuts at: 1563 3258 3631 1563
Size: 1695 373 3063
Fragments arranged by size:
3063 1695 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 3194 4578 4846 1632
Size: 492 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1070 492 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4934
AflIII A'CryG_T
Cuts at: 1203 2133 4030 5007 1203
Size: 930 1897 977 1327
Fragments arranged by size:
1897 1327 977 930
AgeI A'CCGG_T
Cuts at: 3962 3962
Size: 5131
AhdI GACnn_n'nnGTC
Cuts at: 3860 3860
Size: 5131
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5131
AlwNI CAG_nnn'CTG
Cuts at: 2874 4446 2874
Size: 1572 3559
ApaLI G'TGCA_C
Cuts at: 3188 4344 3188
Size: 1156 3975
ApoI r'AATT_y
Cuts at: 1493 2440 2666 2818 4927 1493
Size: 947 226 152 2109 1697
Fragments arranged by size:
2109 1697 947 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3685 160
Size: 872 2653 1606
Fragments arranged by size:
2653 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2456 1870
Size: 73 112 401 4545
Fragments arranged by size:
4545 401 112 73
BamHI G'GATC_C
Cuts at: 2461 2828 2461
Size: 367 4764
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 3907 618
Size: 325 217 627 665 1455 1842
Fragments arranged by size:
1842 1455 665 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2124 2450 1391
Size: 661 72 326 4072
Fragments arranged by size:
4072 661 326 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 928
Size: 971 294 3866
Fragments arranged by size:
3866 971 294
BbvCI CC'TCA_GC
Cuts at: 2082 2082
Size: 5131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3322 3356 1903
Size: 34 1385 34 3678
Fragments arranged by size:
3678 1385 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3023 4233 3023
Size: 1210 3921
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5131
BfrBI ATG'CAT
Cuts at: 2384 2384
Size: 5131
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3742 136
Size: 107 122 71 631 605 337 1733 1525
Fragments arranged by size:
1733 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5126 5126
Size: 5131
BlpI GC'TnA_GC
Cuts at: 2548 2548
Size: 5131
Bme1580I G_kGCm'C
Cuts at: 1790 3192 4348 1790
Size: 1402 1156 2573
Fragments arranged by size:
2573 1402 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2172 2862 3820
Size: 416 53 83 399 24 755 690 958
Cuts at: 3820 442
Size: 1753
Fragments arranged by size:
1753 958 755 690 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 3791 1239
Size: 1202 1350 2579
Fragments arranged by size:
2579 1350 1202
Bpu10I CC'TnA_GC
Cuts at: 2082 2082
Size: 5131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 3256 4121 4419 2378
Size: 878 865 298 3090
Fragments arranged by size:
3090 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3794 882
Size: 617 2295 2219
Fragments arranged by size:
2295 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2922 493
Size: 711 1718 2702
Fragments arranged by size:
2702 1718 711
BsaBI GATnn'nnATC
Cuts at: 5125 5125
Size: 5131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2938 3320 3955 275
Size: 53 83 186 936 1405 382 635 1451
Fragments arranged by size:
1451 1405 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3564 3962 4236 4383 4794 3564
Size: 398 274 147 411 3901
Fragments arranged by size:
3901 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 1178
Size: 30 165 30 720 30 4156
Fragments arranged by size:
4156 720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5131
BseYI C'CCAG_C
Cuts at: 4334 4830 4334
Size: 496 4635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2581 3342 4370 1540
Size: 275 186 580 761 1028 2301
Fragments arranged by size:
2301 1028 761 580 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 3192 3277 4348 2124
Size: 1068 85 1071 2907
Fragments arranged by size:
2907 1071 1068 85
BsmI GAATG_Cn'
Cuts at: 2642 2735 2642
Size: 93 5038
BsmBI CGTCTCn'nnnn_
Cuts at: 2052 2052
Size: 5131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2124 2450 3192 3277 4348 1391
Size: 399 262 72 326 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 326 262 85 72
BspEI T'CCGG_A
Cuts at: 4794 4794
Size: 5131
BspHI T'CATG_A
Cuts at: 3020 4750 3020
Size: 1730 3401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2335 2486 844
Size: 1491 151 3489
Fragments arranged by size:
3489 1491 151
BsrBI CCG'CTC
Cuts at: 1394 1823 3018 1394
Size: 429 1195 3507
Fragments arranged by size:
3507 1195 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3626 3800 65
Size: 1183 2378 174 1396
Fragments arranged by size:
2378 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3775 3962 1700
Size: 110 1965 187 2869
Fragments arranged by size:
2869 1965 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5131
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5131
BssSI C'ACGA_G
Cuts at: 3191 4203 3191
Size: 1012 4119
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5131
BstBI TT'CG_AA
Cuts at: 3935 3935
Size: 5131
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5131
BstYI r'GATC_y
Cuts at: 1972 2461 2828 3213 3230 4682 4768 4780 5126
Size: 489 367 385 17 1452 86 12 346
Cuts at: 5126 1972
Size: 1977
Fragments arranged by size:
1977 1452 489 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 3574
Fragments arranged by size:
3574 939 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4392
Fragments arranged by size:
4392 683 56
BtsI GCAGTG_nn'
Cuts at: 2647 3441 3461 2647
Size: 794 20 4317
Fragments arranged by size:
4317 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5096
DraI TTT'AAA
Cuts at: 2435 2435
Size: 5131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4138 809
Size: 507 2822 1802
Fragments arranged by size:
2822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2175 2578
Size: 54 1425 50 275 18 318 27 403
Cuts at: 2578 3467 5096 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 403 318 275 54 50
43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2578 1537
Size: 275 766 4090
Fragments arranged by size:
4090 766 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 3061 1353
Size: 981 727 3423
Fragments arranged by size:
3423 981 727
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3718 4092 4238 387
Size: 1064 138 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2122
Size: 5131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2441 3194 3791 4578 4846 1239
Size: 393 492 317 753 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 492 393 317 268
EcoRI G'AATT_C
Cuts at: 2440 2818 2440
Size: 378 4753
FspI TGC'GCA
Cuts at: 3637 3637
Size: 5131
HaeII r_GCGC'y
Cuts at: 1678 4278 1678
Size: 2600 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 3772 3804 3846
Size: 32 135 32 551 32 1617 32 42
Cuts at: 3846 3878 1373
Size: 32 2626
Fragments arranged by size:
2626 1617 551 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 2721 669
Size: 988 281 537 246 3079
Fragments arranged by size:
3079 988 537 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5131
HpaI GTT'AAC
Cuts at: 2721 2721
Size: 5131
KpnI G_GTAC'C
Cuts at: 2456 2456
Size: 5131
MfeI C'AATT_G
Cuts at: 2730 2730
Size: 5131
MluI A'CGCG_T
Cuts at: 5007 5007
Size: 5131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 4245 4429 1305
Size: 1053 1887 184 2007
Fragments arranged by size:
2007 1887 1053 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 10
Size: 54 1425 343 318 2991
Fragments arranged by size:
2991 1425 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 3448 3607 518
Size: 648 1923 359 159 2042
Fragments arranged by size:
2042 1923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2547 3224 4372 4617 1606
Size: 475 466 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 475 466 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3640
Fragments arranged by size:
3640 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5131
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5131
NotI GC'GGCC_GC
Cuts at: 2578 2578
Size: 5131
NsiI A_TGCA'T
Cuts at: 2386 2386
Size: 5131
NspI r_CATG'y
Cuts at: 1132 1229 2137 2489 4034 1132
Size: 97 908 352 1545 2229
Fragments arranged by size:
2229 1545 908 352 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5131
PciI A'CATG_T
Cuts at: 2133 4030 2133
Size: 1897 3234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5131
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 5131
PmeI GTTT'AAAC
Cuts at: 2435 2435
Size: 5131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 4713 4745 3200
Size: 32 1481 32 3586
Fragments arranged by size:
3586 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5131
PsiI TTA'TAA
Cuts at: 2701 2701
Size: 5131
PstI C_TGCA'G
Cuts at: 830 2310 2483 830
Size: 1480 173 3478
Fragments arranged by size:
3478 1480 173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5131
PvuII CAG'CTG
Cuts at: 2081 2081
Size: 5131
SacI G_AGCT'C
Cuts at: 2124 2124
Size: 5131
SalI G'TCGA_C
Cuts at: 1936 2473 1936
Size: 537 4594
SapI GCTCTTCn'nnn_
Cuts at: 2334 2334
Size: 5131
SbfI CC_TGCA'GG
Cuts at: 2483 2483
Size: 5131
ScaI AGT'ACT
Cuts at: 3379 5091 3379
Size: 1712 3419
SfcI C'TryA_G
Cuts at: 826 1045 2306 2479 3614 4295 4486 826
Size: 219 1261 173 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 681 219 191 173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5131
SmaI CCC'GGG
Cuts at: 1872 2458 1872
Size: 586 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 3235 4136 4398 820
Size: 197 926 414 878 901 262 1553
Fragments arranged by size:
1553 926 901 878 414 262 197
SnaBI TAC'GTA
Cuts at: 493 2922 493
Size: 2429 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5131
SphI G_CATG'C
Cuts at: 1132 2489 1132
Size: 1357 3774
SspI AAT'ATT
Cuts at: 5 52 3055 5
Size: 47 3003 2081
Fragments arranged by size:
3003 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5131
StyI C'CwwG_G
Cuts at: 513 1065 2004 2570 513
Size: 552 939 566 3074
Fragments arranged by size:
3074 939 566 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 3175 3328 3513 3958 325
Size: 1255 1578 17 153 185 445 1498
Fragments arranged by size:
1578 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 3377 5089
Size: 274 80 33 51 1443 432 968 1712
Cuts at: 5089 96
Size: 138
Fragments arranged by size:
1712 1443 968 432 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5091 5123 5091
Size: 32 5099
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5131
XbaI T'CTAG_A
Cuts at: 2467 2467
Size: 5131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2297 2297
Size: 5131
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5131
XmaI C'CCGG_G
Cuts at: 1870 2456 1870
Size: 586 4545
XmnI GAAnn'nnTTC
Cuts at: 3260 3260
Size: 5131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2939 276
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI