Restriction map for
pFN21AB8224
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeII
MaeIII MamI MfeI MflI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AfaI 243 GTAC GT^AC
AlwNI 749 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 556 GGGCCC GGGCC^C
AspI 492 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 215 GGCGCC G^G[CT][GA]CC
BbeI 215 GGCGCC GGCGC^C
BbsI 655 GAAGAC GAAGAC(2/6)
BfaI 253 CTAG C^TAG
BglI 558 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 655 GAAGAC GAAGAC(2/6)
BseRI 46 GAGGAG GAGGAG(10/8)
BsmBI 124 GAGACG CGTCTC(1/5)
BspHI 359 TCATGA T^CATGA
BspMI 279 GCAGGT ACCTGC(4/8)
BsrBI 289 GAGCGG CCGCTC(-3/-3)
BsrDI 461 GCAATG GCAATG(2/0)
BstAPI 604 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 410 CCATTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 273 GATC ^GATC
EcoT14I 26 CCATGG C^C[AT][AT]GG
HgaI 596 GACGC GACGC(5/10)
KasI 215 GGCGCC G^GCGCC
MaeI 253 CTAG C^TAG
MboI 273 GATC ^GATC
MspA1I 220 CAGCGG C[AC]G^C[GT]G
NarI 215 GGCGCC GG^CGCC
NcoI 26 CCATGG C^CATGG
NdeII 273 GATC ^GATC
PflFI 492 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 293 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 337 CTGCAG CTGCA^G
RcaI 359 TCATGA T^CATGA
RsaI 243 GTAC GT^AC
Sau3AI 273 GATC ^GATC
SfaNI 445 GATGC GCATC(5/9)
SfcI 337 CTGCAG C^T[GA][CT]AG
SfoI 215 GGCGCC GGC^GCC
SmaI 200 CCCGGG CCC^GGG
StyI 26 CCATGG C^C[AT][AT]GG
TaqI 275 TCGA T^CGA
TfiI 688 GATTC G^A[AT]TC
Tth111I 492 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 275 TCGA T^CGA
XcmI 26 CCATGGCAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 200 CCCGGG C^CCGGG
XmaI 200 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 215 GGCGCC G[GA]^CG[CT]C
596 GACGCC
AluI 119 AGCT AG^CT
626 AGCT
AvaI 200 CCCGGG C^[CT]CG[GA]G
704 CTCGGG
BalI 99 TGGCCA TGG^CCA
740 TGGCCA
BanII 166 GGGCTC G[GA]GC[CT]^C
556 GGGCCC
BsaHI 215 GGCGCC G[GA]^CG[CT]C
596 GACGCC
BsmAI 124 GAGAC GTCTC(1/5)
490 GAGAC
BsmFI 22 GGGAC GGGAC(10/14)
498 GTCCC
BsoBI 200 CCCGGG C^[CT]CG[GA]G
704 CTCGGG
EaeI 99 TGGCCA [CT]^GGCC[GA]
740 TGGCCA
Hin1I 215 GGCGCC G[GA]^CG[CT]C
596 GACGCC
MluNI 99 TGGCCA TGG^CCA
740 TGGCCA
MscI 99 TGGCCA TGG^CCA
740 TGGCCA
MseI 229 TTAA T^TAA
644 TTAA
MslI 360 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
408 CACCATTGTG
NlaIII 27 CATG CATG^
360 CATG
PflMI 102 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
586 CCACGGGATGG
Tru9I 229 TTAA T^TAA
644 TTAA
Tsp509I 5 AATT ^AATT
227 AATT
TspRI 494 CACTG CA[GC]TG[ACGT][ACGT]^
561 CAGTG
Van91I 102 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
586 CCACGGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AvaII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboII MnlI MspI
MvaI MwoI NciI NlaIV PleI
Sau96I ScrFI TseI