(Circular) (Six-base) MAPSORT of: pFN21AB8224.seq Check: 1761 from: 1 to: 5464 pFN21AB8224 5464 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2275 2275 Size: 5464 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3527 4911 5179 1632 Size: 1895 1384 268 1917 Fragments arranged by size: 1917 1895 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 2417 1166 Size: 1251 4213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5464 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2343 2374 2375 2342 Size: 1 31 1 5431 Fragments arranged by size: 5431 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2759 3207 4779 2759 Size: 448 1572 3444 Fragments arranged by size: 3444 1572 448 ApaI G_GGCC'C Cuts at: 2565 2565 Size: 5464 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2205 2709 2789 1870 Size: 73 262 504 80 4545 Fragments arranged by size: 4545 504 262 80 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2220 2785 4240 618 Size: 325 217 627 433 565 1455 1842 Fragments arranged by size: 1842 1455 627 565 433 325 217 BanII G_rGCy'C Cuts at: 1391 2175 2565 2783 1391 Size: 784 390 218 4072 Fragments arranged by size: 4072 784 390 218 BbeI G_GCGC'C Cuts at: 2224 2224 Size: 5464 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2667 928 Size: 971 768 3725 Fragments arranged by size: 3725 971 768 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2569 4075 136 Size: 107 122 71 631 605 897 1506 1525 Fragments arranged by size: 1525 1506 897 631 605 122 107 71 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 2447 2565 3525 4681 1790 Size: 657 118 960 1156 2573 Fragments arranged by size: 2573 1156 960 657 118 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2559 3195 4153 Size: 416 53 83 399 24 1142 636 958 Cuts at: 4153 442 Size: 1753 Fragments arranged by size: 1753 1142 958 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2074 2349 2074 Size: 275 5189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2147 2444 2469 2610 4124 1239 Size: 896 12 297 25 141 1514 2579 Fragments arranged by size: 2579 1514 896 297 141 25 12 Bpu10I CC'TnA_GC Cuts at: 2167 2167 Size: 5464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3589 4454 4752 3589 Size: 865 298 4301 Fragments arranged by size: 4301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4127 882 Size: 617 2628 2219 Fragments arranged by size: 2628 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2221 2602 3271 3653 Size: 53 83 186 936 688 381 669 382 Cuts at: 3653 4288 275 Size: 635 1451 Fragments arranged by size: 1451 936 688 669 635 382 381 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 1178 Size: 30 165 30 729 30 4480 Fragments arranged by size: 4480 729 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 4584 BseYI C'CCAG_C Cuts at: 2545 2752 4667 5163 2545 Size: 207 1915 496 2846 Fragments arranged by size: 2846 1915 496 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 2508 2663 1163 Size: 573 606 166 155 3964 Fragments arranged by size: 3964 606 573 166 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3525 3610 4681 3525 Size: 85 1071 4308 Fragments arranged by size: 4308 1071 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 BsmBI CGTCTCn'nnnn_ Cuts at: 2123 2123 Size: 5464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2447 2565 2783 3525 3610 4681 Size: 399 385 272 118 218 742 85 1071 Cuts at: 4681 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 385 272 218 118 85 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 2364 3353 5083 2364 Size: 989 1730 2745 Fragments arranged by size: 2745 1730 989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2275 2819 844 Size: 1431 544 3489 Fragments arranged by size: 3489 1431 544 BsrBI CCG'CTC Cuts at: 1394 1823 2296 3351 1394 Size: 429 473 1055 3507 Fragments arranged by size: 3507 1055 473 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2473 3959 4133 65 Size: 1183 1225 1486 174 1396 Fragments arranged by size: 1486 1396 1225 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4108 4295 1700 Size: 110 2298 187 2869 Fragments arranged by size: 2869 2298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 2615 794 Size: 1821 3643 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2422 2011 Size: 411 5053 BstYI r'GATC_y Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459 Size: 822 367 385 17 1452 86 12 346 Cuts at: 5459 1972 Size: 1977 Fragments arranged by size: 1977 1452 822 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2040 2591 513 Size: 552 939 27 9 551 3386 Fragments arranged by size: 3386 939 552 551 27 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2980 3774 3794 2980 Size: 794 20 4650 Fragments arranged by size: 4650 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2745 2911 Size: 54 1425 50 275 18 274 641 166 Cuts at: 2911 3800 5429 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 641 275 274 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2190 2570 3394 1353 Size: 831 6 380 824 3423 Fragments arranged by size: 3423 831 824 380 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2587 4051 4425 4571 387 Size: 1064 138 998 1464 374 146 1280 Fragments arranged by size: 1464 1280 1064 998 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2147 2444 2469 2610 3527 4124 Size: 393 503 12 297 25 141 917 597 Cuts at: 4124 4911 5179 1239 Size: 787 268 1524 Fragments arranged by size: 1524 917 787 597 503 393 297 268 141 25 12 EcoO109I rG'GnC_Cy Cuts at: 2299 2561 2686 2299 Size: 262 125 5077 Fragments arranged by size: 5077 262 125 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 2066 2224 2269 4611 1678 Size: 388 158 45 2342 2531 Fragments arranged by size: 2531 2342 388 158 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2486 2518 4105 Size: 32 135 32 560 32 322 32 1587 Cuts at: 4105 4137 4179 4211 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1587 560 322 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KasI G'GCGC_C Cuts at: 2220 2220 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 2747 10 Size: 54 1425 343 274 641 2727 Fragments arranged by size: 2727 1425 641 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2369 2417 3422 3781 3940 518 Size: 648 1203 48 1005 359 159 2042 Fragments arranged by size: 2042 1203 1005 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2227 2880 3557 4705 4950 1606 Size: 621 653 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 653 621 245 NarI GG'CG_CC Cuts at: 2221 2221 Size: 5464 NcoI C'CATG_G Cuts at: 513 1065 2004 2031 513 Size: 552 939 27 3946 Fragments arranged by size: 3946 939 552 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2822 4367 1132 Size: 97 1593 1545 2229 Fragments arranged by size: 2229 1593 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5464 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 2597 1108 Size: 1005 484 3975 Fragments arranged by size: 3975 1005 484 PfoI T'CCnGG_A Cuts at: 2112 2175 2112 Size: 63 5401 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2299 Size: 5464 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PspOMI G'GGCC_C Cuts at: 2561 2561 Size: 5464 PstI C_TGCA'G Cuts at: 830 2346 2816 830 Size: 1516 470 3478 Fragments arranged by size: 3478 1516 470 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2342 2812 3947 4628 4819 826 Size: 219 1297 470 1135 681 191 1471 Fragments arranged by size: 1471 1297 1135 681 470 219 191 SfoI GGC'GCC Cuts at: 2222 2222 Size: 5464 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2207 2791 1872 Size: 335 584 4545 Fragments arranged by size: 4545 584 335 SmlI C'TyrA_G Cuts at: 820 1017 1943 3568 4469 4731 820 Size: 197 926 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5464 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2903 513 Size: 552 939 27 872 3074 Fragments arranged by size: 3074 939 872 552 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3491 3508 3661 3846 4291 325 Size: 1255 1911 17 153 185 445 1498 Fragments arranged by size: 1911 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3710 5422 96 Size: 274 80 33 51 1443 1733 1712 138 Fragments arranged by size: 1733 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 2500 1316 Size: 1184 4280 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2038 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2205 2789 1870 Size: 335 584 4545 Fragments arranged by size: 4545 584 335 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI