Restriction map for
pFN21AB8224
(Linear) (Six-base) MAPSORT of: KIBB8224.seq Check: 82 from: 1 to: 759
KIBB8224.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 270 759
Size: 270 489
AleI CACnn'nnGTG
Cuts at: 0 412 759
Size: 412 347
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 338 369 370 759
Size: 337 1 31 1 389
Fragments arranged by size:
389 337 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 754 759
Size: 754 5
ApaI G_GGCC'C
Cuts at: 0 560 759
Size: 560 199
AvaI C'yCGr_G
Cuts at: 0 200 704 759
Size: 200 504 55
Fragments arranged by size:
504 200 55
BanI G'GyrC_C
Cuts at: 0 215 759
Size: 215 544
BanII G_rGCy'C
Cuts at: 0 170 560 759
Size: 170 390 199
Fragments arranged by size:
390 199 170
BbeI G_GCGC'C
Cuts at: 0 219 759
Size: 219 540
BbsI GAAGACnn'nnnn_
Cuts at: 0 662 759
Size: 662 97
BglI GCCn_nnn'nGGC
Cuts at: 0 564 759
Size: 564 195
Bme1580I G_kGCm'C
Cuts at: 0 442 560 759
Size: 442 118 199
Fragments arranged by size:
442 199 118
BmrI ACTGGGnnnn_n'
Cuts at: 0 554 759
Size: 554 205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 69 344 759
Size: 69 275 415
Fragments arranged by size:
415 275 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 142 439 464 605 759
Size: 130 12 297 25 141 154
Fragments arranged by size:
297 154 141 130 25 12
Bpu10I CC'TnA_GC
Cuts at: 0 162 759
Size: 162 597
BsaHI Gr'CG_yC
Cuts at: 0 216 597 759
Size: 216 381 162
Fragments arranged by size:
381 216 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 759
Size: 127 30 602
Fragments arranged by size:
602 127 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 759
Size: 61 698
BseYI C'CCAG_C
Cuts at: 0 540 747 759
Size: 540 207 12
Fragments arranged by size:
540 207 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 503 658 759
Size: 337 166 155 101
Fragments arranged by size:
337 166 155 101
BsmBI CGTCTCn'nnnn_
Cuts at: 0 118 759
Size: 118 641
Bsp1286I G_dGCh'C
Cuts at: 0 170 442 560 759
Size: 170 272 118 199
Fragments arranged by size:
272 199 170 118
BspHI T'CATG_A
Cuts at: 0 359 759
Size: 359 400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 270 759
Size: 270 489
BsrBI CCG'CTC
Cuts at: 0 291 759
Size: 291 468
BsrDI GCAATG_nn'
Cuts at: 0 468 759
Size: 468 291
BstAPI GCAn_nnn'nTGC
Cuts at: 0 610 759
Size: 610 149
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 759
Size: 417 342
BtgI C'CryG_G
Cuts at: 0 26 35 586 759
Size: 26 9 551 173
Fragments arranged by size:
551 173 26 9
EaeI y'GGCC_r
Cuts at: 0 99 740 759
Size: 99 641 19
Fragments arranged by size:
641 99 19
EarI CTCTTCn'nnn_
Cuts at: 0 179 185 565 759
Size: 179 6 380 194
Fragments arranged by size:
380 194 179 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 582 759
Size: 582 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 142 439 464 605 759
Size: 130 12 297 25 141 154
Fragments arranged by size:
297 154 141 130 25 12
EcoO109I rG'GnC_Cy
Cuts at: 0 294 556 681 759
Size: 294 262 125 78
Fragments arranged by size:
294 262 125 78
HaeII r_GCGC'y
Cuts at: 0 61 219 264 759
Size: 61 158 45 495
Fragments arranged by size:
495 158 61 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 481 513 759
Size: 127 32 322 32 246
Fragments arranged by size:
322 246 127 32 32
KasI G'GCGC_C
Cuts at: 0 215 759
Size: 215 544
MscI TGG'CCA
Cuts at: 0 101 742 759
Size: 101 641 17
Fragments arranged by size:
641 101 17
MslI CAynn'nnrTG
Cuts at: 0 364 412 759
Size: 364 48 347
Fragments arranged by size:
364 347 48
MspA1I CmG'CkG
Cuts at: 0 222 759
Size: 222 537
NarI GG'CG_CC
Cuts at: 0 216 759
Size: 216 543
NcoI C'CATG_G
Cuts at: 0 26 759
Size: 26 733
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 592 759
Size: 108 484 167
Fragments arranged by size:
484 167 108
PfoI T'CCnGG_A
Cuts at: 0 107 170 759
Size: 107 63 589
Fragments arranged by size:
589 107 63
PpuMI rG'GwC_Cy
Cuts at: 0 294 759
Size: 294 465
PspOMI G'GGCC_C
Cuts at: 0 556 759
Size: 556 203
PstI C_TGCA'G
Cuts at: 0 341 759
Size: 341 418
SfcI C'TryA_G
Cuts at: 0 337 759
Size: 337 422
SfoI GGC'GCC
Cuts at: 0 217 759
Size: 217 542
SmaI CCC'GGG
Cuts at: 0 202 759
Size: 202 557
StyI C'CwwG_G
Cuts at: 0 26 759
Size: 26 733
Tth111I GACn'n_nGTC
Cuts at: 0 495 759
Size: 495 264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 759
Size: 33 726
XmaI C'CCGG_G
Cuts at: 0 200 759
Size: 200 559
Enzymes that do cut:
AarI AleI AloI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI Bme1580I BmrI BplI BpmI Bpu10I
BsaHI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BstAPI BstXI BtgI EaeI EarI
EciI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NarI NcoI PflMI PfoI PpuMI PspOMI PstI
SfcI SfoI SmaI StyI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI
BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI XbaI XhoI XmnI ZraI