(Circular) (Six-base) MAPSORT of: pFN21AB8219.seq Check: 1913 from: 1 to: 5656 pFN21AB8219 5656 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2566 2566 Size: 5656 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2628 3719 5103 5371 1632 Size: 996 1091 1384 268 1917 Fragments arranged by size: 1917 1384 1091 996 268 AflII C'TTAA_G Cuts at: 820 1017 2048 820 Size: 197 1031 4428 Fragments arranged by size: 4428 1031 197 AflIII A'CryG_T Cuts at: 1203 2304 4555 5532 1203 Size: 1101 2251 977 1327 Fragments arranged by size: 2251 1327 1101 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2333 2679 3399 4971 2333 Size: 346 720 1572 3018 Fragments arranged by size: 3018 1572 720 346 ApaI G_GGCC'C Cuts at: 2400 2400 Size: 5656 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2393 2399 2981 1870 Size: 73 450 6 582 4545 Fragments arranged by size: 4545 582 450 73 6 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2205 2323 2345 2453 2977 Size: 325 217 627 418 118 22 108 524 Cuts at: 2977 4432 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 524 418 325 217 118 108 22 BanII G_rGCy'C Cuts at: 1391 2114 2400 2975 1391 Size: 723 286 575 4072 Fragments arranged by size: 4072 723 575 286 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2022 2411 928 Size: 971 123 389 4173 Fragments arranged by size: 4173 971 389 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3847 3881 1903 Size: 34 50 34 1826 34 3678 Fragments arranged by size: 3678 1826 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 2484 1548 Size: 936 4720 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2810 2831 2846 Size: 107 122 71 631 605 1138 21 15 Cuts at: 2846 4267 136 Size: 1421 1525 Fragments arranged by size: 1525 1421 1138 631 605 122 107 71 21 15 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2208 2400 2456 3717 4873 1790 Size: 418 192 56 1261 1156 2573 Fragments arranged by size: 2573 1261 1156 418 192 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 2425 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2431 2514 2672 4316 1239 Size: 1010 182 83 158 1644 2579 Fragments arranged by size: 2579 1644 1010 182 158 83 Bpu10I CC'TnA_GC Cuts at: 2892 2892 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2396 2951 4319 882 Size: 617 897 555 1368 2219 Fragments arranged by size: 2219 1368 897 617 555 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 2073 5650 2073 Size: 3577 2079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2487 2517 2851 2881 1178 Size: 30 165 30 1084 30 334 30 3953 Fragments arranged by size: 3953 1084 334 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2933 1186 Size: 1747 3909 BseYI C'CCAG_C Cuts at: 2221 2887 4859 5355 2221 Size: 666 1972 496 2522 Fragments arranged by size: 2522 1972 666 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 1163 Size: 573 762 4321 Fragments arranged by size: 4321 762 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3717 3802 4873 3717 Size: 85 1071 4500 Fragments arranged by size: 4500 1071 85 BsmI GAATG_Cn' Cuts at: 2638 3167 3260 2638 Size: 529 93 5034 Fragments arranged by size: 5034 529 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2759 2759 Size: 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2208 2400 2456 2975 3717 3802 Size: 399 324 94 192 56 519 742 85 Cuts at: 3802 4873 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 519 399 324 192 94 85 56 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2566 3011 844 Size: 1722 445 3489 Fragments arranged by size: 3489 1722 445 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2349 2671 4151 4325 65 Size: 1183 1101 322 1480 174 1396 Fragments arranged by size: 1480 1396 1183 1101 322 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstXI CCAn_nnnn'nTGG Cuts at: 2387 2387 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2130 2986 3353 3738 3755 5207 5293 5305 Size: 158 856 367 385 17 1452 86 12 Cuts at: 5305 5651 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 856 385 367 346 158 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 3172 3966 3986 3172 Size: 794 20 4842 Fragments arranged by size: 4842 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2032 4663 809 Size: 507 716 2631 1802 Fragments arranged by size: 2631 1802 716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 3103 3992 Size: 54 1425 50 275 18 483 790 889 Cuts at: 3992 5621 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 790 483 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2904 3586 1353 Size: 1551 682 3423 Fragments arranged by size: 3423 1551 682 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2431 2514 2628 2672 3719 4316 Size: 393 617 182 83 114 44 1047 597 Cuts at: 4316 5103 5371 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1047 787 617 597 393 268 182 114 83 44 EcoO109I rG'GnC_Cy Cuts at: 2284 2284 Size: 5656 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 EcoRV GAT'ATC Cuts at: 2620 2620 Size: 5656 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2428 2824 2428 Size: 396 5260 FspI TGC'GCA Cuts at: 2189 4162 2189 Size: 1973 3683 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2931 2963 4297 4329 4371 Size: 32 135 32 1359 32 1334 32 42 Cuts at: 4371 4403 1373 Size: 32 2626 Fragments arranged by size: 2626 1359 1334 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 2083 2895 2904 4770 4954 1305 Size: 756 22 812 9 1866 184 2007 Fragments arranged by size: 2007 1866 812 756 184 22 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 10 Size: 54 1425 343 483 3351 Fragments arranged by size: 3351 1425 483 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 3614 3973 4132 518 Size: 648 1017 1431 359 159 2042 Fragments arranged by size: 2042 1431 1017 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2330 2482 2864 3072 3749 4897 5142 1606 Size: 724 152 382 208 677 1148 245 2120 Fragments arranged by size: 2120 1148 724 677 382 245 208 152 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5104 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2140 2308 2536 3014 4559 1132 Size: 97 911 168 228 478 1545 2229 Fragments arranged by size: 2229 1545 911 478 228 168 97 PasI CC'CwG_GG Cuts at: 1348 2010 1348 Size: 662 4994 PciI A'CATG_T Cuts at: 2304 4555 2304 Size: 2251 3405 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 1108 Size: 957 4699 PfoI T'CCnGG_A Cuts at: 2649 2649 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2284 Size: 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PspOMI G'GGCC_C Cuts at: 2396 2396 Size: 5656 PstI C_TGCA'G Cuts at: 830 2688 2884 3008 830 Size: 1858 196 124 3478 Fragments arranged by size: 3478 1858 196 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2330 2482 2864 2330 Size: 152 382 5122 Fragments arranged by size: 5122 382 152 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2684 2880 3004 4139 4820 5011 826 Size: 219 1639 196 124 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 681 219 196 191 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 3760 4661 4923 820 Size: 197 926 105 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 262 197 105 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 2804 1517 Size: 1287 4369 StyI C'CwwG_G Cuts at: 513 1065 2277 2904 3095 513 Size: 552 1212 627 191 3074 Fragments arranged by size: 3074 1212 627 552 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2487 2519 2851 2883 5616 5648 2487 Size: 32 332 32 2733 32 2495 Fragments arranged by size: 2733 2495 332 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2084 3785 2084 Size: 1701 3955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI