(Circular) (Six-base) MAPSORT of: pFN21AB8214.seq Check: 4898 from: 1 to: 5476 pFN21AB8214 5476 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 2599 Size: 5476 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 3603 3976 1563 Size: 2040 373 3063 Fragments arranged by size: 3063 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3539 4923 5191 1632 Size: 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 4375 5352 1203 Size: 3172 977 1327 Fragments arranged by size: 3172 1327 977 AgeI A'CCGG_T Cuts at: 2715 4307 2715 Size: 1592 3884 AhdI GACnn_n'nnGTC Cuts at: 4205 4205 Size: 5476 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 2518 2659 3219 4791 2518 Size: 141 560 1572 3203 Fragments arranged by size: 3203 1572 560 141 ApaI G_GGCC'C Cuts at: 2250 2250 Size: 5476 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2249 2264 2801 1870 Size: 73 306 15 537 4545 Fragments arranged by size: 4545 537 306 73 15 BamHI G'GATC_C Cuts at: 2487 2806 3173 2487 Size: 319 367 4790 Fragments arranged by size: 4790 367 319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2315 2351 2561 2797 Size: 325 217 627 279 249 36 210 236 Cuts at: 2797 4252 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 279 249 236 217 210 36 BanII G_rGCy'C Cuts at: 1391 2102 2250 2256 2702 2795 1391 Size: 711 148 6 446 93 4072 Fragments arranged by size: 4072 711 446 148 93 6 BbeI G_GCGC'C Cuts at: 2319 2355 2565 2319 Size: 36 210 5230 Fragments arranged by size: 5230 210 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4505 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2183 4087 136 Size: 107 122 71 631 605 511 1904 1525 Fragments arranged by size: 1904 1525 631 605 511 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2893 2893 Size: 5476 Bme1580I G_kGCm'C Cuts at: 1790 2081 2250 2439 3537 4693 1790 Size: 291 169 189 1098 1156 2573 Fragments arranged by size: 2573 1156 1098 291 189 169 BmgBI CAC'GTC Cuts at: 1318 2434 1318 Size: 1116 4360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4165 442 Size: 416 53 83 399 24 1790 958 1753 Fragments arranged by size: 1790 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 2681 4136 1239 Size: 1307 135 1455 2579 Fragments arranged by size: 2579 1455 1307 135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4139 882 Size: 617 2640 2219 Fragments arranged by size: 2640 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2554 3267 493 Size: 711 1350 713 2702 Fragments arranged by size: 2702 1350 713 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2352 2562 3283 Size: 53 83 186 936 783 36 210 721 Cuts at: 3283 3665 4300 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 783 721 635 382 210 186 83 53 36 BsaWI w'CCGG_w Cuts at: 2631 2715 3909 4307 4581 4728 5139 2631 Size: 84 1194 398 274 147 411 2968 Fragments arranged by size: 2968 1194 411 398 274 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5251 Fragments arranged by size: 5251 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2678 1186 Size: 1492 3984 BseYI C'CCAG_C Cuts at: 2168 2311 2355 4679 5175 2168 Size: 143 44 2324 496 2469 Fragments arranged by size: 2469 2324 496 143 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 1163 Size: 573 705 4198 Fragments arranged by size: 4198 705 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 2178 2214 2926 3687 4715 Size: 275 186 120 57 36 712 761 1028 Cuts at: 4715 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 712 275 186 120 57 36 BsiHKAI G_wGCw'C Cuts at: 2256 3537 3622 4693 2256 Size: 1281 85 1071 3039 Fragments arranged by size: 3039 1281 1071 85 BsmI GAATG_Cn' Cuts at: 2987 3080 2987 Size: 93 5383 BsmBI CGTCTCn'nnnn_ Cuts at: 2597 2597 Size: 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2102 2250 2256 2439 2702 2795 Size: 399 291 21 148 6 183 263 93 Cuts at: 2795 3537 3622 4693 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 291 263 183 148 93 85 21 6 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2599 2831 844 Size: 1755 232 3489 Fragments arranged by size: 3489 1755 232 BsrBI CCG'CTC Cuts at: 1394 1823 2137 2232 2569 3363 1394 Size: 429 314 95 337 794 3507 Fragments arranged by size: 3507 794 429 337 314 95 BsrDI GCAATG_nn' Cuts at: 65 1248 2724 3971 4145 65 Size: 1183 1476 1247 174 1396 Fragments arranged by size: 1476 1396 1247 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2069 2582 2715 4120 4307 1700 Size: 110 259 513 133 1405 187 2869 Fragments arranged by size: 2869 1405 513 259 187 133 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 2530 1925 Size: 605 4871 BssSI C'ACGA_G Cuts at: 2255 3536 4548 2255 Size: 1281 1012 3183 Fragments arranged by size: 3183 1281 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2518 794 Size: 1724 3752 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2487 2806 3173 3558 3575 5027 5113 5125 Size: 515 319 367 385 17 1452 86 12 Cuts at: 5125 5471 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 515 385 367 346 319 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 2145 2208 2217 513 Size: 552 951 129 63 9 3772 Fragments arranged by size: 3772 951 552 129 63 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3786 3806 2992 Size: 794 20 4662 Fragments arranged by size: 4662 794 20 ClaI AT'CG_AT Cuts at: 1554 2757 1554 Size: 1203 4273 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DraIII CAC_nnn'GTG Cuts at: 2554 2554 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2759 4483 809 Size: 507 1443 1724 1802 Fragments arranged by size: 1802 1724 1443 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2052 2118 Size: 54 1425 50 275 18 189 33 66 Cuts at: 2118 2175 2211 2410 2580 2923 3812 5441 8 Size: 57 36 199 170 343 889 1629 43 Fragments arranged by size: 1629 1425 889 343 275 199 189 170 66 57 54 50 43 36 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2118 2175 2211 2923 1537 Size: 275 306 57 36 712 4090 Fragments arranged by size: 4090 712 306 275 57 36 EarI CTCTTCn'nnn_ Cuts at: 1353 3406 1353 Size: 2053 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4063 4437 4583 387 Size: 1064 138 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2254 2254 Size: 5476 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2546 2681 3539 4136 4923 5191 1239 Size: 393 914 135 858 597 787 268 1524 Fragments arranged by size: 1524 914 858 787 597 393 268 135 EcoO109I rG'GnC_Cy Cuts at: 2365 2389 2672 2365 Size: 24 283 5169 Fragments arranged by size: 5169 283 24 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 5476 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 2153 2319 2355 2565 4623 1678 Size: 475 166 36 210 2058 2531 Fragments arranged by size: 2531 2058 475 210 166 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2281 2313 2750 2782 4117 Size: 32 135 32 709 32 437 32 1335 Cuts at: 4117 4149 4191 4223 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1335 709 437 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KasI G'GCGC_C Cuts at: 2315 2351 2561 2315 Size: 36 210 5230 Fragments arranged by size: 5230 210 36 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4774 1305 Size: 3285 184 2007 Fragments arranged by size: 3285 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2054 10 Size: 54 1425 343 222 3432 Fragments arranged by size: 3432 1425 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 3434 3793 3952 518 Size: 648 2268 359 159 2042 Fragments arranged by size: 2268 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2210 2219 2311 2326 2515 2578 2892 Size: 412 192 9 92 15 189 63 314 Cuts at: 2892 3569 4717 4962 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 412 314 245 192 189 92 63 15 9 NaeI GCC'GGC Cuts at: 2584 2584 Size: 5476 NarI GG'CG_CC Cuts at: 2316 2352 2562 2316 Size: 36 210 5230 Fragments arranged by size: 5230 210 36 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4924 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NgoMIV G'CCGG_C Cuts at: 2582 2582 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2118 2211 2923 2118 Size: 93 712 4671 Fragments arranged by size: 4671 712 93 NspI r_CATG'y Cuts at: 1132 1229 2834 4379 1132 Size: 97 1605 1545 2229 Fragments arranged by size: 2229 1605 1545 97 PasI CC'CwG_GG Cuts at: 1348 2385 1348 Size: 1037 4439 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 2110 2554 1108 Size: 1002 444 4030 Fragments arranged by size: 4030 1002 444 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2243 2275 3545 3577 5058 5090 2243 Size: 32 1270 32 1481 32 2629 Fragments arranged by size: 2629 1481 1270 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2365 2389 2365 Size: 24 5452 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PspOMI G'GGCC_C Cuts at: 2246 2246 Size: 5476 PstI C_TGCA'G Cuts at: 830 2331 2828 830 Size: 1501 497 3478 Fragments arranged by size: 3478 1501 497 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 PvuII CAG'CTG Cuts at: 2311 2515 2578 2311 Size: 204 63 5209 Fragments arranged by size: 5209 204 63 SacI G_AGCT'C Cuts at: 2256 2256 Size: 5476 SacII CC_GC'GG Cuts at: 2019 2211 2220 2019 Size: 192 9 5275 Fragments arranged by size: 5275 192 9 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SanDI GG'GwC_CC Cuts at: 2365 2365 Size: 5476 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2327 2824 3959 4640 4831 826 Size: 219 1282 497 1135 681 191 1471 Fragments arranged by size: 1471 1282 1135 681 497 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2183 2183 Size: 5476 SfoI GGC'GCC Cuts at: 2317 2353 2563 2317 Size: 36 210 5230 Fragments arranged by size: 5230 210 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 2069 1700 Size: 369 5107 SmaI CCC'GGG Cuts at: 1872 2803 1872 Size: 931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3580 4481 4743 820 Size: 197 926 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5476 StyI C'CwwG_G Cuts at: 513 1065 2046 2055 2915 513 Size: 552 981 9 860 3074 Fragments arranged by size: 3074 981 860 552 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3503 3520 3673 3858 4303 325 Size: 1255 1923 17 153 185 445 1498 Fragments arranged by size: 1923 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2643 3722 5434 Size: 274 80 33 51 1443 666 1079 1712 Cuts at: 5434 96 Size: 138 Fragments arranged by size: 1712 1443 1079 666 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5436 5468 5436 Size: 32 5444 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 2044 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2801 1870 Size: 931 4545 XmnI GAAnn'nnTTC Cuts at: 2402 3605 2402 Size: 1203 4273 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SrfI SwaI