Restriction map for
pFN21AB8211
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 797 ACATGT A^C[GA][CT]GT
AspHI 244 GAGCAC G[AT]GC[AT]^C
BalI 551 TGGCCA TGG^CCA
BamHI 916 GGATCC G^GATCC
BbrPI 907 CACGTG CAC^GTG
BbsI 196 GTCTTC GAAGAC(2/6)
BfaI 764 CTAG C^TAG
BpuAI 196 GTCTTC GAAGAC(2/6)
BsaAI 907 CACGTG [CT]AC^GT[GA]
BsaWI 718 ACCGGA [AT]^CCGG[AT]
BsiHKAI 244 GAGCAC G[AT]GC[AT]^C
Bsp1407I 800 TGTACA T^GTACA
BspHI 809 TCATGA T^CATGA
BspLU11I 797 ACATGT A^CATGT
BspMI 83 ACCTGC ACCTGC(4/8)
BsrBI 850 CCGCTC CCGCTC(-3/-3)
BsrGI 800 TGTACA T^GTACA
BsrI 671 CCAGT ACTGG(1/-1)
DraIII 904 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 117 GACCTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 430 CCTCCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 972 GGCCGGCC GGCCGG^CC
KspI 1000 CCGCGG CCGC^GG
MaeI 764 CTAG C^TAG
MluNI 551 TGGCCA TGG^CCA
MscI 551 TGGCCA TGG^CCA
MspA1I 1000 CCGCGG C[AC]G^C[GT]G
NotI 1021 GCGGCCGC GC^GGCCGC
PleI 423 GAGTC GAGTC(4/5)
PmaCI 907 CACGTG CAC^GTG
PmlI 907 CACGTG CAC^GTG
PstI 284 CTGCAG CTGCA^G
RcaI 809 TCATGA T^CATGA
SacII 1000 CCGCGG CCGC^GG
SfiI 862 GGCCCGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 990 CCCGGG CCC^GGG
SphI 584 GCATGC GCATG^C
SspBI 800 TGTACA T^GTACA
TaqI 682 TCGA T^CGA
TfiI 775 GATTC G^A[AT]TC
Tsp509I 844 AATT ^AATT
TspRI 672 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 682 TCGA T^CGA
XmaCI 990 CCCGGG C^CCGGG
XmaI 990 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BpmI 786 CTCCAG CTGGAG(16/14)
770 CTGGAG
BsiEI 26 CGGCCG CG[GA][CT]^CG
1022 CGGCCG
BssSI 488 CACGAG CACGAG(-5/-1)
542 CTCGTG
BstXI 567 CCAGAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
629 CCATGAACATGG
BstYI 131 AGATCC [GA]^GATC[CT]
916 GGATCC
DdeI 128 CTGAG C^T[ACGT]AG
979 CTGAG
EagI 26 CGGCCG C^GGCCG
1022 CGGCCG
EarI 230 GAAGAG CTCTTC(1/4)
951 GAAGAG
EclXI 26 CGGCCG C^GGCCG
1022 CGGCCG
Eco52I 26 CGGCCG C^GGCCG
1022 CGGCCG
HgaI 534 GACGC GACGC(5/10)
194 GCGTC
HinfI 423 GAGTC G^A[ACGT]TC
775 GATTC
Ksp632I 230 GAAGAG CTCTTC(1/4)
951 GAAGAG
MaeII 71 ACGT A^CGT
908 ACGT
MflI 131 AGATCC [GA]^GATC[CT]
916 GGATCC
NspI 584 GCATGC [GA]CATG^[CT]
797 ACATGT
PpuMI 203 GGGACCT [GA]G^G[AT]CC[CT]
1030 GGGACCT
SfaNI 417 GATGC GCATC(5/9)
501 GCATC
SfcI 276 CTATAG C^T[GA][CT]AG
284 CTGCAG
XcmI 296 CCAGCCCCGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
884 CCATGAGCGCGATGG
XhoII 131 AGATCC [GA]^GATC[CT]
916 GGATCC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MaeIII
MboI MboII MnlI MslI MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfoI StuI StyI TseI Tsp45I