(Circular) (Six-base) MAPSORT of: pFN21AB8211.seq Check: 5743 from: 1 to: 5794 pFN21AB8211 5794 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3137 1937 Size: 1200 4594 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3857 5241 5509 1632 Size: 2225 1384 268 1917 Fragments arranged by size: 2225 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5597 AflIII A'CryG_T Cuts at: 1203 2802 4693 5670 1203 Size: 1599 1891 977 1327 Fragments arranged by size: 1891 1599 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 2100 1166 Size: 934 4860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2118 1184 Size: 934 4860 AlwNI CAG_nnn'CTG Cuts at: 3537 5109 3537 Size: 1572 4222 ApaI G_GGCC'C Cuts at: 2405 2571 2648 2748 2879 2405 Size: 166 77 100 131 5320 Fragments arranged by size: 5320 166 131 100 77 ApaLI G'TGCA_C Cuts at: 3851 5007 3851 Size: 1156 4638 ApoI r'AATT_y Cuts at: 1493 3103 3329 3481 5590 1493 Size: 1610 226 152 2109 1697 Fragments arranged by size: 2109 1697 1610 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 2932 2995 3055 3119 1870 Size: 73 989 63 60 64 4545 Fragments arranged by size: 4545 989 73 64 63 60 BamHI G'GATC_C Cuts at: 2921 3124 3491 2921 Size: 203 367 5224 Fragments arranged by size: 5224 367 203 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2224 2474 2834 2969 Size: 325 217 627 315 122 250 360 135 Cuts at: 2969 2999 3115 4570 618 Size: 30 116 1455 1842 Fragments arranged by size: 1842 1455 627 360 325 315 250 217 135 122 116 30 BanII G_rGCy'C Cuts at: 1391 2172 2405 2487 2500 2571 2648 2748 2879 Size: 781 233 82 13 71 77 100 131 Cuts at: 2879 2933 3113 1391 Size: 54 180 4072 Fragments arranged by size: 4072 781 233 180 131 100 82 77 71 54 13 BbeI G_GCGC'C Cuts at: 2228 2478 2838 2973 2228 Size: 250 360 135 5049 Fragments arranged by size: 5049 360 250 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2194 928 Size: 971 295 4528 Fragments arranged by size: 4528 971 295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3985 4019 1903 Size: 34 2048 34 3678 Fragments arranged by size: 3678 2048 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 1548 Size: 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2451 2843 2874 Size: 107 122 71 631 605 779 392 31 Cuts at: 2874 4405 136 Size: 1531 1525 Fragments arranged by size: 1531 1525 779 631 605 392 122 107 71 31 BglII A'GATC_T Cuts at: 5789 5789 Size: 5794 BlpI GC'TnA_GC Cuts at: 3211 3211 Size: 5794 Bme1580I G_kGCm'C Cuts at: 1790 2040 2107 2405 2571 2648 2748 2879 3002 Size: 250 67 298 166 77 100 131 123 Cuts at: 3002 3855 5011 1790 Size: 853 1156 2573 Fragments arranged by size: 2573 1156 853 298 250 166 131 123 100 77 67 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2670 3525 4483 Size: 416 53 83 399 24 1253 855 958 Cuts at: 4483 442 Size: 1753 Fragments arranged by size: 1753 1253 958 855 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2451 2451 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2776 2796 4454 1239 Size: 1537 20 1658 2579 Fragments arranged by size: 2579 1658 1537 20 Bpu10I CC'TnA_GC Cuts at: 2984 2984 Size: 5794 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3919 4784 5082 3919 Size: 865 298 4631 Fragments arranged by size: 4631 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4457 882 Size: 617 2958 2219 Fragments arranged by size: 2958 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2914 3585 493 Size: 711 1710 671 2702 Fragments arranged by size: 2702 1710 711 671 BsaBI GATnn'nnATC Cuts at: 5788 5788 Size: 5794 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2225 2475 2540 2835 Size: 53 83 186 936 692 250 65 295 Cuts at: 2835 2970 3601 3983 4618 275 Size: 135 631 382 635 1451 Fragments arranged by size: 1451 936 692 635 631 382 295 250 186 135 83 65 53 BsaWI w'CCGG_w Cuts at: 2723 4227 4625 4899 5046 5457 2723 Size: 1504 398 274 147 411 3060 Fragments arranged by size: 3060 1504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5569 Fragments arranged by size: 5569 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2535 2689 2692 2751 1186 Size: 1270 79 154 3 59 4229 Fragments arranged by size: 4229 1270 154 79 59 3 BseYI C'CCAG_C Cuts at: 2161 2300 2457 4997 5493 2161 Size: 139 157 2540 496 2462 Fragments arranged by size: 2540 2462 496 157 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2078 2168 3029 1163 Size: 573 342 90 861 3928 Fragments arranged by size: 3928 861 573 342 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3030 3244 4005 5033 1540 Size: 275 186 33 996 214 761 1028 2301 Fragments arranged by size: 2301 1028 996 761 275 214 186 33 BsiHKAI G_wGCw'C Cuts at: 2253 3855 3940 5011 2253 Size: 1602 85 1071 3036 Fragments arranged by size: 3036 1602 1071 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2107 2172 2253 2405 2487 2500 Size: 399 250 67 65 81 152 82 13 Cuts at: 2500 2571 2648 2748 2879 2933 3002 3113 3855 Size: 71 77 100 131 54 69 111 742 Cuts at: 3855 3940 5011 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 250 152 131 111 100 85 82 81 77 71 69 67 65 54 13 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 2814 3683 5413 2814 Size: 869 1730 3195 Fragments arranged by size: 3195 1730 869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 3149 844 Size: 1253 1052 3489 Fragments arranged by size: 3489 1253 1052 BsrBI CCG'CTC Cuts at: 1394 1823 2857 3681 1394 Size: 429 1034 824 3507 Fragments arranged by size: 3507 1034 824 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4289 4463 65 Size: 1183 3041 174 1396 Fragments arranged by size: 3041 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2615 2837 2978 4438 4625 1700 Size: 110 805 222 141 1460 187 2869 Fragments arranged by size: 2869 1460 805 222 187 141 110 BsrGI T'GTAC_A Cuts at: 96 2805 96 Size: 2709 3085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 2493 2547 3854 4866 2493 Size: 54 1307 1012 3421 Fragments arranged by size: 3421 1307 1012 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5794 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5794 BstXI CCAn_nnnn'nTGG Cuts at: 2579 2641 3091 2579 Size: 62 450 5282 Fragments arranged by size: 5282 450 62 BstYI r'GATC_y Cuts at: 1972 2136 2921 3124 3491 3876 3893 5345 5431 Size: 164 785 203 367 385 17 1452 86 Cuts at: 5431 5443 5789 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 785 385 367 346 203 164 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2352 2478 2663 2880 2973 3005 513 Size: 552 1287 126 185 217 93 32 3302 Fragments arranged by size: 3302 1287 552 217 185 126 93 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2912 487 Size: 683 56 1686 3369 Fragments arranged by size: 3369 1686 683 56 BtsI GCAGTG_nn' Cuts at: 3310 4104 4124 3310 Size: 794 20 4980 Fragments arranged by size: 4980 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5759 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DraIII CAC_nnn'GTG Cuts at: 2914 2914 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2128 4801 809 Size: 507 812 2673 1802 Fragments arranged by size: 2673 1802 812 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2340 2556 Size: 54 1425 50 275 18 201 309 216 Cuts at: 2556 2883 2976 3027 3241 4130 5759 8 Size: 327 93 51 214 889 1629 43 Fragments arranged by size: 1629 1425 889 327 309 275 216 214 201 93 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3027 3241 1537 Size: 275 219 996 214 4090 Fragments arranged by size: 4090 996 275 219 214 EarI CTCTTCn'nnn_ Cuts at: 1353 2230 2951 3724 1353 Size: 877 721 773 3423 Fragments arranged by size: 3423 877 773 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4381 4755 4901 387 Size: 1064 138 2792 374 146 1280 Fragments arranged by size: 2792 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2776 2796 3857 4454 5241 5509 1239 Size: 393 1144 20 1061 597 787 268 1524 Fragments arranged by size: 1524 1144 1061 787 597 393 268 20 EcoNI CCTnn'n_nnAGG Cuts at: 2439 2439 Size: 5794 EcoO109I rG'GnC_Cy Cuts at: 2209 2401 2402 2567 2568 2745 2875 2876 3036 Size: 192 1 165 1 177 130 1 160 Cuts at: 3036 2209 Size: 4967 Fragments arranged by size: 4967 192 177 165 160 130 1 1 1 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 FseI GG_CCGG'CC Cuts at: 2982 2982 Size: 5794 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 2072 2228 2478 2838 2973 4941 1678 Size: 394 156 250 360 135 1968 2531 Fragments arranged by size: 2531 1968 394 360 250 156 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2413 2445 2530 2562 2890 Size: 32 135 32 841 32 85 32 328 Cuts at: 2890 2922 4435 4467 4509 4541 1373 Size: 32 1513 32 42 32 2626 Fragments arranged by size: 2626 1513 841 328 135 85 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3138 3384 669 Size: 988 281 1200 246 3079 Fragments arranged by size: 3079 1200 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KasI G'GCGC_C Cuts at: 2224 2474 2834 2969 2224 Size: 250 360 135 5049 Fragments arranged by size: 5049 360 250 135 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 4908 5092 1305 Size: 762 2841 184 2007 Fragments arranged by size: 2841 2007 762 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2558 10 Size: 54 1425 343 726 3246 Fragments arranged by size: 3246 1425 726 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2100 2639 2813 3089 3752 4111 4270 Size: 648 934 539 174 276 663 359 159 Cuts at: 4270 518 Size: 2042 Fragments arranged by size: 2042 934 663 648 539 359 276 174 159 MspA1I CmG'CkG Cuts at: 1606 3007 3210 3887 5035 5280 1606 Size: 1401 203 677 1148 245 2120 Fragments arranged by size: 2120 1401 1148 677 245 203 NaeI GCC'GGC Cuts at: 2617 2839 2980 2617 Size: 222 141 5431 Fragments arranged by size: 5431 222 141 NarI GG'CG_CC Cuts at: 2225 2475 2835 2970 2225 Size: 250 360 135 5049 Fragments arranged by size: 5049 360 250 135 NcoI C'CATG_G Cuts at: 513 1065 2352 2478 2880 513 Size: 552 1287 126 402 3427 Fragments arranged by size: 3427 1287 552 402 126 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NgoMIV G'CCGG_C Cuts at: 2615 2837 2978 2615 Size: 222 141 5431 Fragments arranged by size: 5431 222 141 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3027 3241 3027 Size: 214 5580 NspI r_CATG'y Cuts at: 1132 1229 2593 2806 3152 4697 1132 Size: 97 1364 213 346 1545 2229 Fragments arranged by size: 2229 1545 1364 346 213 97 PasI CC'CwG_GG Cuts at: 1348 2174 2396 2500 2926 1348 Size: 826 222 104 426 4216 Fragments arranged by size: 4216 826 426 222 104 PciI A'CATG_T Cuts at: 2802 4693 2802 Size: 1891 3903 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5794 PfoI T'CCnGG_A Cuts at: 2274 2274 Size: 5794 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PmlI CAC'GTG Cuts at: 2914 2914 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3863 3895 5376 5408 3863 Size: 32 1481 32 4249 Fragments arranged by size: 4249 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 3036 2209 Size: 827 4967 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5794 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspOMI G'GGCC_C Cuts at: 2401 2567 2644 2744 2875 2401 Size: 166 77 100 131 5320 Fragments arranged by size: 5320 166 131 100 77 PstI C_TGCA'G Cuts at: 830 2293 3146 830 Size: 1463 853 3478 Fragments arranged by size: 3478 1463 853 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 SacII CC_GC'GG Cuts at: 3008 3008 Size: 5794 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SfcI C'TryA_G Cuts at: 826 1045 2281 2289 3142 4277 4958 5149 826 Size: 219 1236 8 853 1135 681 191 1471 Fragments arranged by size: 1471 1236 1135 853 681 219 191 8 SfiI GGCCn_nnn'nGGCC Cuts at: 2874 2874 Size: 5794 SfoI GGC'GCC Cuts at: 2226 2476 2836 2971 2226 Size: 250 360 135 5049 Fragments arranged by size: 5049 360 250 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5794 SmaI CCC'GGG Cuts at: 1872 2997 3121 1872 Size: 1125 124 4545 Fragments arranged by size: 4545 1125 124 SmlI C'TyrA_G Cuts at: 820 1017 1943 3898 4799 5061 820 Size: 197 926 1955 901 262 1553 Fragments arranged by size: 1955 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 2593 3152 1132 Size: 1461 559 3774 Fragments arranged by size: 3774 1461 559 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 2243 2270 2445 2625 1517 Size: 726 27 175 180 4686 Fragments arranged by size: 4686 726 180 175 27 StyI C'CwwG_G Cuts at: 513 1065 2013 2265 2352 2478 2880 3233 513 Size: 552 948 252 87 126 402 353 3074 Fragments arranged by size: 3074 948 552 402 353 252 126 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2617 2773 3821 3838 3991 4176 4621 Size: 1255 1037 156 1048 17 153 185 445 Cuts at: 4621 325 Size: 1498 Fragments arranged by size: 1498 1255 1048 1037 445 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 2805 4040 Size: 274 80 33 51 1443 237 591 1235 Cuts at: 4040 5752 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1235 591 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2376 2408 5754 5786 2376 Size: 32 3346 32 2384 Fragments arranged by size: 3346 2384 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5794 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2308 2896 2308 Size: 588 5206 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5794 XmaI C'CCGG_G Cuts at: 1870 2995 3119 1870 Size: 1125 124 4545 Fragments arranged by size: 4545 1125 124 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI