Restriction map for
pFN21AB8211
(Linear) (Six-base) MAPSORT of: KIBB8211.seq Check: 425 from: 1 to: 1089
KIBB8211.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AflIII A'CryG_T
Cuts at: 0 797 1089
Size: 797 292
AleI CACnn'nnGTG
Cuts at: 0 95 1089
Size: 95 994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 113 1089
Size: 113 976
ApaI G_GGCC'C
Cuts at: 0 400 566 643 743 874 1089
Size: 400 166 77 100 131 215
Fragments arranged by size:
400 215 166 131 100 77
AvaI C'yCGr_G
Cuts at: 0 927 990 1050 1089
Size: 927 63 60 39
Fragments arranged by size:
927 63 60 39
BamHI G'GATC_C
Cuts at: 0 916 1089
Size: 916 173
BanI G'GyrC_C
Cuts at: 0 97 219 469 829 964 994 1089
Size: 97 122 250 360 135 30 95
Fragments arranged by size:
360 250 135 122 97 95 30
BanII G_rGCy'C
Cuts at: 0 167 400 482 495 566 643 743 874
Size: 167 233 82 13 71 77 100 131
Cuts at: 874 928 1089
Size: 54 161
Fragments arranged by size:
233 167 161 131 100 82 77 71
54 13
BbeI G_GCGC'C
Cuts at: 0 223 473 833 968 1089
Size: 223 250 360 135 121
Fragments arranged by size:
360 250 223 135 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 189 1089
Size: 189 900
BglI GCCn_nnn'nGGC
Cuts at: 0 446 838 869 1089
Size: 446 392 31 220
Fragments arranged by size:
446 392 220 31
Bme1580I G_kGCm'C
Cuts at: 0 35 102 400 566 643 743 874 997
Size: 35 67 298 166 77 100 131 123
Cuts at: 997 1089
Size: 92
Fragments arranged by size:
298 166 131 123 100 92 77 67
35
BmrI ACTGGGnnnn_n'
Cuts at: 0 665 1089
Size: 665 424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 446 1089
Size: 446 643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 791 1089
Size: 771 20 298
Fragments arranged by size:
771 298 20
Bpu10I CC'TnA_GC
Cuts at: 0 979 1089
Size: 979 110
BsaAI yAC'GTr
Cuts at: 0 909 1089
Size: 909 180
BsaHI Gr'CG_yC
Cuts at: 0 220 470 535 830 965 1089
Size: 220 250 65 295 135 124
Fragments arranged by size:
295 250 220 135 124 65
BsaWI w'CCGG_w
Cuts at: 0 718 1089
Size: 718 371
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 530 684 687 746 1089
Size: 451 79 154 3 59 343
Fragments arranged by size:
451 343 154 79 59 3
BseYI C'CCAG_C
Cuts at: 0 156 295 452 1089
Size: 156 139 157 637
Fragments arranged by size:
637 157 156 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 163 1024 1089
Size: 73 90 861 65
Fragments arranged by size:
861 90 73 65
BsiEI CG_ry'CG
Cuts at: 0 29 1025 1089
Size: 29 996 64
Fragments arranged by size:
996 64 29
BsiHKAI G_wGCw'C
Cuts at: 0 248 1089
Size: 248 841
Bsp1286I G_dGCh'C
Cuts at: 0 35 102 167 248 400 482 495 566
Size: 35 67 65 81 152 82 13 71
Cuts at: 566 643 743 874 928 997 1089
Size: 77 100 131 54 69 92
Fragments arranged by size:
152 131 100 92 82 81 77 71
69 67 65 54 35 13
BspHI T'CATG_A
Cuts at: 0 809 1089
Size: 809 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 1089
Size: 92 997
BsrBI CCG'CTC
Cuts at: 0 852 1089
Size: 852 237
BsrFI r'CCGG_y
Cuts at: 0 610 832 973 1089
Size: 610 222 141 116
Fragments arranged by size:
610 222 141 116
BsrGI T'GTAC_A
Cuts at: 0 800 1089
Size: 800 289
BssSI C'ACGA_G
Cuts at: 0 488 542 1089
Size: 488 54 547
Fragments arranged by size:
547 488 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 574 636 1089
Size: 574 62 453
Fragments arranged by size:
574 453 62
BstYI r'GATC_y
Cuts at: 0 131 916 1089
Size: 131 785 173
Fragments arranged by size:
785 173 131
BtgI C'CryG_G
Cuts at: 0 347 473 658 875 968 1000 1089
Size: 347 126 185 217 93 32 89
Fragments arranged by size:
347 217 185 126 93 89 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 907 1089
Size: 907 182
DraIII CAC_nnn'GTG
Cuts at: 0 909 1089
Size: 909 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 123 1089
Size: 123 966
EaeI y'GGCC_r
Cuts at: 0 26 335 551 878 971 1022 1089
Size: 26 309 216 327 93 51 67
Fragments arranged by size:
327 309 216 93 67 51 26
EagI C'GGCC_G
Cuts at: 0 26 1022 1089
Size: 26 996 67
Fragments arranged by size:
996 67 26
EarI CTCTTCn'nnn_
Cuts at: 0 225 946 1089
Size: 225 721 143
Fragments arranged by size:
721 225 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 791 1089
Size: 771 20 298
Fragments arranged by size:
771 298 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 434 1089
Size: 434 655
EcoO109I rG'GnC_Cy
Cuts at: 0 204 396 397 562 563 740 870 871
Size: 204 192 1 165 1 177 130 1
Cuts at: 871 1031 1089
Size: 160 58
Fragments arranged by size:
204 192 177 165 160 130 58 1
1 1
FseI GG_CCGG'CC
Cuts at: 0 977 1089
Size: 977 112
HaeII r_GCGC'y
Cuts at: 0 67 223 473 833 968 1089
Size: 67 156 250 360 135 121
Fragments arranged by size:
360 250 156 135 121 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 408 440 525 557 885 917 1089
Size: 408 32 85 32 328 32 172
Fragments arranged by size:
408 328 172 85 32 32 32
KasI G'GCGC_C
Cuts at: 0 219 469 829 964 1089
Size: 219 250 360 135 125
Fragments arranged by size:
360 250 219 135 125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1089
Size: 62 1027
MscI TGG'CCA
Cuts at: 0 553 1089
Size: 553 536
MslI CAynn'nnrTG
Cuts at: 0 95 634 808 1084 1089
Size: 95 539 174 276 5
Fragments arranged by size:
539 276 174 95 5
MspA1I CmG'CkG
Cuts at: 0 1002 1089
Size: 1002 87
NaeI GCC'GGC
Cuts at: 0 612 834 975 1089
Size: 612 222 141 114
Fragments arranged by size:
612 222 141 114
NarI GG'CG_CC
Cuts at: 0 220 470 830 965 1089
Size: 220 250 360 135 124
Fragments arranged by size:
360 250 220 135 124
NcoI C'CATG_G
Cuts at: 0 347 473 875 1089
Size: 347 126 402 214
Fragments arranged by size:
402 347 214 126
NgoMIV G'CCGG_C
Cuts at: 0 610 832 973 1089
Size: 610 222 141 116
Fragments arranged by size:
610 222 141 116
NotI GC'GGCC_GC
Cuts at: 0 1022 1089
Size: 1022 67
NspI r_CATG'y
Cuts at: 0 588 801 1089
Size: 588 213 288
Fragments arranged by size:
588 288 213
PasI CC'CwG_GG
Cuts at: 0 169 391 495 921 1089
Size: 169 222 104 426 168
Fragments arranged by size:
426 222 169 168 104
PciI A'CATG_T
Cuts at: 0 797 1089
Size: 797 292
PfoI T'CCnGG_A
Cuts at: 0 269 1089
Size: 269 820
PmlI CAC'GTG
Cuts at: 0 909 1089
Size: 909 180
PpuMI rG'GwC_Cy
Cuts at: 0 204 1031 1089
Size: 204 827 58
Fragments arranged by size:
827 204 58
PspOMI G'GGCC_C
Cuts at: 0 396 562 639 739 870 1089
Size: 396 166 77 100 131 219
Fragments arranged by size:
396 219 166 131 100 77
PstI C_TGCA'G
Cuts at: 0 288 1089
Size: 288 801
SacII CC_GC'GG
Cuts at: 0 1003 1089
Size: 1003 86
SfcI C'TryA_G
Cuts at: 0 276 284 1089
Size: 276 8 805
Fragments arranged by size:
805 276 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 869 1089
Size: 869 220
SfoI GGC'GCC
Cuts at: 0 221 471 831 966 1089
Size: 221 250 360 135 123
Fragments arranged by size:
360 250 221 135 123
SmaI CCC'GGG
Cuts at: 0 992 1089
Size: 992 97
SphI G_CATG'C
Cuts at: 0 588 1089
Size: 588 501
StuI AGG'CCT
Cuts at: 0 238 265 440 620 1089
Size: 238 27 175 180 469
Fragments arranged by size:
469 238 180 175 27
StyI C'CwwG_G
Cuts at: 0 8 260 347 473 875 1089
Size: 8 252 87 126 402 214
Fragments arranged by size:
402 252 214 126 87 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 612 768 1089
Size: 612 156 321
Fragments arranged by size:
612 321 156
TatI w'GTAC_w
Cuts at: 0 209 800 1089
Size: 209 591 289
Fragments arranged by size:
591 289 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 371 403 1089
Size: 371 32 686
Fragments arranged by size:
686 371 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 891 1089
Size: 303 588 198
Fragments arranged by size:
588 303 198
XmaI C'CCGG_G
Cuts at: 0 990 1089
Size: 990 99
Enzymes that do cut:
AflIII AleI AlfI ApaI AvaI BamHI BanI BanII
BbeI BbsI BglI Bme1580I BmrI BplI BpmI Bpu10I
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstXI
BstYI BtgI BtgZI DraIII DrdI EaeI EagI EarI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PciI PfoI PmlI PpuMI PspOMI PstI
SacII SfcI SfiI SfoI SmaI SphI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaXI
BsiWI BsmI BsmBI BspEI BsrDI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI EciI
EcoICRI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NruI NsiI
PacI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI