(Circular) (Six-base) MAPSORT of: pFN21AB8210.seq Check: 9457 from: 1 to: 5833 pFN21AB8210 5833 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3896 5280 5548 1632 Size: 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AlwNI CAG_nnn'CTG Cuts at: 3576 5148 3576 Size: 1572 4261 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2708 2852 2972 3142 3368 3520 5629 1493 Size: 1215 144 120 170 226 152 2109 1697 Fragments arranged by size: 2109 1697 1215 226 170 152 144 120 AseI AT'TA_AT Cuts at: 160 1032 2228 4387 160 Size: 872 1196 2159 1606 Fragments arranged by size: 2159 1606 1196 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3154 4609 618 Size: 325 217 627 1367 1455 1842 Fragments arranged by size: 1842 1455 1367 627 325 217 BanII G_rGCy'C Cuts at: 1391 2587 3152 1391 Size: 1196 565 4072 Fragments arranged by size: 4072 1196 565 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4024 4058 1903 Size: 34 2087 34 3678 Fragments arranged by size: 3678 2087 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BfrBI ATG'CAT Cuts at: 2116 2116 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2265 4444 136 Size: 107 122 71 631 605 593 2179 1525 Fragments arranged by size: 2179 1525 631 605 593 122 107 71 BglII A'GATC_T Cuts at: 3120 5828 3120 Size: 2708 3125 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 3894 5050 1790 Size: 2104 1156 2573 Fragments arranged by size: 2573 2104 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2291 1056 Size: 1235 4598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2343 2568 4493 1239 Size: 1104 225 1925 2579 Fragments arranged by size: 2579 1925 1104 225 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3958 4823 5121 3958 Size: 865 298 4670 Fragments arranged by size: 4670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4496 882 Size: 617 2997 2219 Fragments arranged by size: 2997 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 2783 5827 2783 Size: 3044 2789 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5608 Fragments arranged by size: 5608 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5833 BseYI C'CCAG_C Cuts at: 2263 2530 5036 5532 2263 Size: 267 2506 496 2564 Fragments arranged by size: 2564 2506 496 267 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 4843 Fragments arranged by size: 4843 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3894 3979 5050 3894 Size: 85 1071 4677 Fragments arranged by size: 4677 1071 85 BsmI GAATG_Cn' Cuts at: 3064 3344 3437 3064 Size: 280 93 5460 Fragments arranged by size: 5460 280 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2587 3152 3894 3979 5050 1391 Size: 399 797 565 742 85 1071 2174 Fragments arranged by size: 2174 1071 797 742 565 399 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 2890 3722 5452 2890 Size: 832 1730 3271 Fragments arranged by size: 3271 1730 832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2545 2786 2992 3188 844 Size: 1331 370 241 206 196 3489 Fragments arranged by size: 3489 1331 370 241 206 196 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2031 2937 4328 4502 65 Size: 1183 783 906 1391 174 1396 Fragments arranged by size: 1396 1391 1183 906 783 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2692 4477 4664 1700 Size: 110 882 1785 187 2869 Fragments arranged by size: 2869 1785 882 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 2702 794 Size: 1908 3925 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2656 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2102 2772 3051 3120 3163 3530 3915 3932 Size: 130 670 279 69 43 367 385 17 Cuts at: 3932 5384 5470 5482 5828 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 670 385 367 346 279 130 86 69 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 2484 513 Size: 552 1419 3862 Fragments arranged by size: 3862 1419 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2117 487 Size: 683 56 891 4203 Fragments arranged by size: 4203 891 683 56 BtsI GCAGTG_nn' Cuts at: 2700 3349 4143 4163 2700 Size: 649 794 20 4370 Fragments arranged by size: 4370 794 649 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 2145 3137 2145 Size: 992 4841 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 3280 4169 Size: 54 1425 50 275 18 816 634 889 Cuts at: 4169 5798 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 816 634 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3763 1353 Size: 2410 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 2568 3896 4493 5280 5548 1239 Size: 393 711 225 1328 597 787 268 1524 Fragments arranged by size: 1524 1328 787 711 597 393 268 225 EcoNI CCTnn'n_nnAGG Cuts at: 3098 3098 Size: 5833 EcoO109I rG'GnC_Cy Cuts at: 2534 2534 Size: 5833 EcoRI G'AATT_C Cuts at: 2852 3142 3520 2852 Size: 290 378 5165 Fragments arranged by size: 5165 378 290 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 2411 2443 2872 Size: 32 135 32 725 32 82 32 429 Cuts at: 2872 2904 4474 4506 4548 4580 1373 Size: 32 1570 32 42 32 2626 Fragments arranged by size: 2626 1570 725 429 135 82 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4947 5131 1305 Size: 3642 184 2007 Fragments arranged by size: 3642 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3791 4150 4309 518 Size: 648 2625 359 159 2042 Fragments arranged by size: 2625 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 3249 3926 5074 5319 1606 Size: 657 986 677 1148 245 2120 Fragments arranged by size: 2120 1148 986 677 657 245 NaeI GCC'GGC Cuts at: 2694 2694 Size: 5833 NcoI C'CATG_G Cuts at: 513 1065 2484 513 Size: 552 1419 3862 Fragments arranged by size: 3862 1419 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NgoMIV G'CCGG_C Cuts at: 2692 2692 Size: 5833 NheI G'CTAG_C Cuts at: 1052 2287 1052 Size: 1235 4598 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NsiI A_TGCA'T Cuts at: 2118 2118 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 2093 1348 Size: 745 5088 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2099 3089 1108 Size: 991 990 3852 Fragments arranged by size: 3852 991 990 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2534 2534 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 2868 3403 2868 Size: 535 5298 PstI C_TGCA'G Cuts at: 830 3185 830 Size: 2355 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2263 2263 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 3181 4316 4997 5188 826 Size: 219 2136 1135 681 191 1471 Fragments arranged by size: 2136 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4838 5100 820 Size: 197 926 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2484 3272 513 Size: 552 1419 788 3074 Fragments arranged by size: 3074 1419 788 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2897 3860 3877 4030 4215 4660 325 Size: 1255 1317 963 17 153 185 445 1498 Fragments arranged by size: 1498 1317 1255 963 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3071 4079 5791 Size: 274 80 33 51 1443 1094 1008 1712 Cuts at: 5791 96 Size: 138 Fragments arranged by size: 1712 1443 1094 1008 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5793 5825 5793 Size: 32 5801 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI