(Circular) (Six-base) MAPSORT of: pFN21AB8209.seq Check: 8847 from: 1 to: 5521 pFN21AB8209 5521 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2377 2864 1937 Size: 440 487 4594 Fragments arranged by size: 4594 487 440 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3584 4968 5236 1632 Size: 1952 1384 268 1917 Fragments arranged by size: 1952 1917 1384 268 AfeI AGC'GCT Cuts at: 2513 2513 Size: 5521 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 2224 4420 5397 1203 Size: 1021 2196 977 1327 Fragments arranged by size: 2196 1327 1021 977 AgeI A'CCGG_T Cuts at: 2247 4352 2247 Size: 2105 3416 AhdI GACnn_n'nnGTC Cuts at: 4250 4250 Size: 5521 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5521 AlwNI CAG_nnn'CTG Cuts at: 2282 2662 3264 4836 2282 Size: 380 602 1572 2967 Fragments arranged by size: 2967 1572 602 380 ApaI G_GGCC'C Cuts at: 2671 2671 Size: 5521 ApaLI G'TGCA_C Cuts at: 3578 4734 3578 Size: 1156 4365 ApoI r'AATT_y Cuts at: 1493 2830 3056 3208 5317 1493 Size: 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 226 152 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2846 1870 Size: 73 903 4545 Fragments arranged by size: 4545 903 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 2842 4297 618 Size: 325 217 627 609 446 1455 1842 Fragments arranged by size: 1842 1455 627 609 446 325 217 BanII G_rGCy'C Cuts at: 1391 2671 2707 2751 2773 2840 1391 Size: 1280 36 44 22 67 4072 Fragments arranged by size: 4072 1280 67 44 36 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2221 928 Size: 971 322 4228 Fragments arranged by size: 4228 971 322 BbvCI CC'TCA_GC Cuts at: 2241 2471 2241 Size: 230 5291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2001 2035 3712 3746 1903 Size: 34 64 34 1677 34 3678 Fragments arranged by size: 3678 1677 64 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2622 4132 Size: 107 122 71 631 605 337 613 1510 Cuts at: 4132 136 Size: 1525 Fragments arranged by size: 1525 1510 631 613 605 337 122 107 71 BglII A'GATC_T Cuts at: 5516 5516 Size: 5521 BlpI GC'TnA_GC Cuts at: 2938 2938 Size: 5521 Bme1580I G_kGCm'C Cuts at: 1790 2401 2671 3582 4738 1790 Size: 611 270 911 1156 2573 Fragments arranged by size: 2573 1156 911 611 270 BmgBI CAC'GTC Cuts at: 1318 2188 2696 1318 Size: 870 508 4143 Fragments arranged by size: 4143 870 508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3252 4210 442 Size: 416 53 83 399 24 1835 958 1753 Fragments arranged by size: 1835 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4181 1239 Size: 2942 2579 Bpu10I CC'TnA_GC Cuts at: 2241 2471 2241 Size: 230 5291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 2517 2536 3646 4511 4809 2423 Size: 94 19 1110 865 298 3135 Fragments arranged by size: 3135 1110 865 298 94 19 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 4184 882 Size: 617 1041 1644 2219 Fragments arranged by size: 2219 1644 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 3312 493 Size: 711 2108 2702 Fragments arranged by size: 2702 2108 711 BsaBI GATnn'nnATC Cuts at: 5515 5515 Size: 5521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3328 3710 4345 275 Size: 53 83 186 936 1795 382 635 1451 Fragments arranged by size: 1795 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 3954 4352 4626 4773 5184 2247 Size: 1707 398 274 147 411 2584 Fragments arranged by size: 2584 1707 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5296 Fragments arranged by size: 5296 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2099 1186 Size: 910 3 4608 Fragments arranged by size: 4608 910 3 BseYI C'CCAG_C Cuts at: 2640 4724 5220 2640 Size: 2084 496 2941 Fragments arranged by size: 2941 2084 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 1163 Size: 573 432 4516 Fragments arranged by size: 4516 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2598 2971 3732 4760 1540 Size: 275 186 597 373 761 1028 2301 Fragments arranged by size: 2301 1028 761 597 373 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 3582 3667 4738 2481 Size: 1101 85 1071 3264 Fragments arranged by size: 3264 1101 1071 85 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 BsmBI CGTCTCn'nnnn_ Cuts at: 2251 2251 Size: 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2401 2481 2671 2707 2751 2773 2840 Size: 399 611 80 190 36 44 22 67 Cuts at: 2840 3582 3667 4738 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 399 190 85 80 67 44 36 22 BspEI T'CCGG_A Cuts at: 5184 5184 Size: 5521 BspHI T'CATG_A Cuts at: 3410 5140 3410 Size: 1730 3791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2876 844 Size: 2032 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2522 2736 3408 1394 Size: 429 699 214 672 3507 Fragments arranged by size: 3507 699 672 429 214 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 4190 65 Size: 1183 2768 174 1396 Fragments arranged by size: 2768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2247 4165 4352 1700 Size: 110 437 1918 187 2869 Fragments arranged by size: 2869 1918 437 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5521 BssSI C'ACGA_G Cuts at: 3581 4593 3581 Size: 1012 4509 BstAPI GCAn_nnn'nTGC Cuts at: 794 2282 794 Size: 1488 4033 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5521 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2365 Size: 5521 BstYI r'GATC_y Cuts at: 1972 2760 2851 3218 3603 3620 5072 5158 5170 Size: 788 91 367 385 17 1452 86 12 Cuts at: 5170 5516 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 788 385 367 346 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4030 Fragments arranged by size: 4030 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4782 Fragments arranged by size: 4782 683 56 BtsI GCAGTG_nn' Cuts at: 3037 3831 3851 3037 Size: 794 20 4707 Fragments arranged by size: 4707 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2825 2825 Size: 5521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 2595 2684 Size: 54 1425 50 275 18 540 225 89 Cuts at: 2684 2754 2968 3857 5486 8 Size: 70 214 889 1629 43 Fragments arranged by size: 1629 1425 889 540 275 225 214 89 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2595 2968 1537 Size: 275 783 373 4090 Fragments arranged by size: 4090 783 373 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3451 1353 Size: 2098 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4108 4482 4628 387 Size: 1064 138 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3584 4181 4968 5236 1239 Size: 393 1952 597 787 268 1524 Fragments arranged by size: 1952 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2605 2667 2798 2605 Size: 62 131 5328 Fragments arranged by size: 5328 131 62 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 FspI TGC'GCA Cuts at: 2504 4027 2504 Size: 1523 3998 HaeII r_GCGC'y Cuts at: 1678 2208 2497 2515 4668 1678 Size: 530 289 18 2153 2531 Fragments arranged by size: 2531 2153 530 289 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4162 4194 4236 4268 1373 Size: 32 135 32 2590 32 42 32 2626 Fragments arranged by size: 2626 2590 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3111 669 Size: 988 281 927 246 3079 Fragments arranged by size: 3079 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 2224 5397 2224 Size: 3173 2348 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2200 2763 4635 4819 1305 Size: 895 563 1872 184 2007 Fragments arranged by size: 2007 1872 895 563 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 10 Size: 54 1425 343 924 2775 Fragments arranged by size: 2775 1425 924 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 3479 3838 3997 518 Size: 648 1224 1089 359 159 2042 Fragments arranged by size: 2042 1224 1089 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2282 2937 3614 4762 5007 1606 Size: 444 232 655 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 655 444 245 232 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4030 Fragments arranged by size: 4030 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2879 4424 1132 Size: 97 1650 1545 2229 Fragments arranged by size: 2229 1650 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5521 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PfoI T'CCnGG_A Cuts at: 2627 2627 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2234 2266 3590 3622 5103 5135 2234 Size: 32 1324 32 1481 32 2620 Fragments arranged by size: 2620 1481 1324 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2605 2798 2605 Size: 193 5328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PspOMI G'GGCC_C Cuts at: 2667 2667 Size: 5521 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2426 2458 2426 Size: 32 5489 PstI C_TGCA'G Cuts at: 830 2873 830 Size: 2043 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 PvuII CAG'CTG Cuts at: 2282 2282 Size: 5521 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SanDI GG'GwC_CC Cuts at: 2605 2605 Size: 5521 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 2444 3769 5481 2444 Size: 1325 1712 2484 Fragments arranged by size: 2484 1712 1325 SexAI A'CCwGG_T Cuts at: 2459 2459 Size: 5521 SfcI C'TryA_G Cuts at: 826 1045 2869 4004 4685 4876 826 Size: 219 1824 1135 681 191 1471 Fragments arranged by size: 1824 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5521 SmaI CCC'GGG Cuts at: 1872 2848 1872 Size: 976 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 2515 2532 3625 4526 4788 Size: 197 926 495 77 17 1093 901 262 Cuts at: 4788 820 Size: 1553 Fragments arranged by size: 1553 1093 926 901 495 262 197 77 17 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 3445 5 Size: 47 3393 2081 Fragments arranged by size: 3393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2004 2310 2960 513 Size: 552 939 306 650 3074 Fragments arranged by size: 3074 939 650 552 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2320 2384 2564 3548 3565 3718 3903 Size: 1255 740 64 180 984 17 153 185 Cuts at: 3903 4348 325 Size: 445 1498 Fragments arranged by size: 1498 1255 984 740 445 185 180 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 3767 5479 Size: 274 80 33 51 1443 465 1325 1712 Cuts at: 5479 96 Size: 138 Fragments arranged by size: 1712 1443 1325 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5481 5513 5481 Size: 32 5489 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2625 2625 Size: 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2846 1870 Size: 976 4545 XmnI GAAnn'nnTTC Cuts at: 3650 3650 Size: 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI