(Circular) (Six-base) MAPSORT of: pFN21AB8204.seq Check: 775 from: 1 to: 6115 pFN21AB8204 6115 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3458 1937 Size: 1521 4594 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2003 2426 2945 2997 3309 4178 5562 5830 Size: 371 423 519 52 312 869 1384 268 Cuts at: 5830 1632 Size: 1917 Fragments arranged by size: 1917 1384 869 519 423 371 312 268 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 2013 4844 2013 Size: 2831 3284 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6115 AlwNI CAG_nnn'CTG Cuts at: 2824 3385 3397 3858 5430 2824 Size: 561 12 461 1572 3509 Fragments arranged by size: 3509 1572 561 461 12 ApaI G_GGCC'C Cuts at: 2099 2099 Size: 6115 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 3424 3650 3802 5911 1493 Size: 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2101 2192 2315 3440 1870 Size: 73 105 53 91 123 1125 4545 Fragments arranged by size: 4545 1125 123 105 91 73 53 BamHI G'GATC_C Cuts at: 2761 3445 3812 2761 Size: 684 367 5064 Fragments arranged by size: 5064 684 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2227 3436 4891 618 Size: 325 217 627 299 141 1209 1455 1842 Fragments arranged by size: 1842 1455 1209 627 325 299 217 141 BanII G_rGCy'C Cuts at: 1391 2068 2099 2108 2166 3434 1391 Size: 677 31 9 58 1268 4072 Fragments arranged by size: 4072 1268 677 58 31 9 BbeI G_GCGC'C Cuts at: 2090 2090 Size: 6115 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2009 2846 3032 928 Size: 971 110 837 186 4011 Fragments arranged by size: 4011 971 837 186 110 BbvCI CC'TCA_GC Cuts at: 2481 2481 Size: 6115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4306 4340 1903 Size: 34 2369 34 3678 Fragments arranged by size: 3678 2369 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2103 4726 136 Size: 107 122 71 631 605 431 2623 1525 Fragments arranged by size: 2623 1525 631 605 431 122 107 71 BglII A'GATC_T Cuts at: 2608 6110 2608 Size: 3502 2613 BlpI GC'TnA_GC Cuts at: 2806 3194 3532 2806 Size: 388 338 5389 Fragments arranged by size: 5389 388 338 Bme1580I G_kGCm'C Cuts at: 1790 2099 2232 4176 5332 1790 Size: 309 133 1944 1156 2573 Fragments arranged by size: 2573 1944 1156 309 133 BmgBI CAC'GTC Cuts at: 1318 3049 1318 Size: 1731 4384 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2743 3846 4804 Size: 416 53 83 399 24 1326 1103 958 Cuts at: 4804 442 Size: 1753 Fragments arranged by size: 1753 1326 1103 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1995 2082 3187 3407 4775 1239 Size: 756 87 1105 220 1368 2579 Fragments arranged by size: 2579 1368 1105 756 220 87 Bpu10I CC'TnA_GC Cuts at: 2481 2481 Size: 6115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3155 3242 4240 5105 5403 3155 Size: 87 998 865 298 3867 Fragments arranged by size: 3867 998 865 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3201 4778 882 Size: 617 1702 1577 2219 Fragments arranged by size: 2219 1702 1577 617 BsaAI yAC'GTr Cuts at: 493 1204 3906 493 Size: 711 2702 2702 Fragments arranged by size: 2702 2702 711 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2087 3922 4304 4939 Size: 53 83 186 936 554 1835 382 635 Cuts at: 4939 275 Size: 1451 Fragments arranged by size: 1835 1451 936 635 554 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4548 4946 5220 5367 5778 4548 Size: 398 274 147 411 4885 Fragments arranged by size: 4885 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2239 2269 1178 Size: 30 165 30 836 30 5024 Fragments arranged by size: 5024 836 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2278 2714 3074 3308 1186 Size: 1092 436 360 234 3993 Fragments arranged by size: 3993 1092 436 360 234 BseYI C'CCAG_C Cuts at: 2546 2782 2943 5318 5814 2546 Size: 236 161 2375 496 2847 Fragments arranged by size: 2847 2375 496 236 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2698 2774 1163 Size: 573 962 76 4504 Fragments arranged by size: 4504 962 573 76 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3565 4326 5354 1540 Size: 275 186 1564 761 1028 2301 Fragments arranged by size: 2301 1564 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3127 4176 4261 5332 3127 Size: 1049 85 1071 3910 Fragments arranged by size: 3910 1071 1049 85 BsmI GAATG_Cn' Cuts at: 3626 3719 3626 Size: 93 6022 BsmBI CGTCTCn'nnnn_ Cuts at: 2660 3140 2660 Size: 480 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2099 2108 2166 2232 3127 3434 Size: 399 278 31 9 58 66 895 307 Cuts at: 3434 4176 4261 5332 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 895 742 399 307 278 85 66 58 31 9 BspEI T'CCGG_A Cuts at: 5778 5778 Size: 6115 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3240 3470 844 Size: 2396 230 3489 Fragments arranged by size: 3489 2396 230 BsrBI CCG'CTC Cuts at: 1394 1823 2805 3240 4002 1394 Size: 429 982 435 762 3507 Fragments arranged by size: 3507 982 762 435 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2499 4610 4784 65 Size: 1183 1251 2111 174 1396 Fragments arranged by size: 2111 1396 1251 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2224 3278 4759 4946 1700 Size: 110 414 1054 1481 187 2869 Fragments arranged by size: 2869 1481 1054 414 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6115 BssSI C'ACGA_G Cuts at: 4175 5187 4175 Size: 1012 5103 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6115 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2608 2761 3138 3174 3363 3445 3812 4197 Size: 636 153 377 36 189 82 367 385 Cuts at: 4197 4214 5666 5752 5764 6110 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 636 385 377 367 346 189 153 86 82 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2034 2091 2151 3099 513 Size: 552 969 57 60 948 3529 Fragments arranged by size: 3529 969 948 552 60 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5376 Fragments arranged by size: 5376 683 56 BtsI GCAGTG_nn' Cuts at: 2260 3631 4425 4445 2260 Size: 1371 794 20 3930 Fragments arranged by size: 3930 1371 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2981 3154 5122 809 Size: 507 1665 173 1968 1802 Fragments arranged by size: 1968 1802 1665 507 173 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3102 3562 4451 Size: 54 1425 50 275 18 1272 460 889 Cuts at: 4451 6080 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1272 889 460 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3562 1537 Size: 275 1750 4090 Fragments arranged by size: 4090 1750 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2402 2575 3285 4045 1353 Size: 1049 173 710 760 3423 Fragments arranged by size: 3423 1049 760 710 173 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4702 5076 5222 387 Size: 1064 138 3113 374 146 1280 Fragments arranged by size: 3113 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2003 2082 2426 2945 2997 3187 Size: 393 363 8 79 344 519 52 190 Cuts at: 3187 3309 3407 4178 4775 5562 5830 1239 Size: 122 98 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 597 519 393 363 344 268 190 122 98 79 52 8 EcoO109I rG'GnC_Cy Cuts at: 2096 2698 2821 3253 2096 Size: 602 123 432 4958 Fragments arranged by size: 4958 602 432 123 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 FspI TGC'GCA Cuts at: 3407 4621 3407 Size: 1214 4901 HaeII r_GCGC'y Cuts at: 1678 2047 2090 2186 2950 3301 5262 1678 Size: 369 43 96 764 351 1961 2531 Fragments arranged by size: 2531 1961 764 369 351 96 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2430 2462 3109 Size: 32 135 32 427 32 399 32 647 Cuts at: 3109 3141 4756 4788 4830 4862 1373 Size: 32 1615 32 42 32 2626 Fragments arranged by size: 2626 1615 647 427 399 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3459 3705 669 Size: 988 281 1521 246 3079 Fragments arranged by size: 3079 1521 988 281 246 HindIII A'AGCT_T Cuts at: 748 3196 748 Size: 2448 3667 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KasI G'GCGC_C Cuts at: 2086 2086 Size: 6115 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 2979 3714 2979 Size: 735 5380 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 2514 5229 5413 1305 Size: 1170 39 2715 184 2007 Fragments arranged by size: 2715 2007 1170 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4293 Fragments arranged by size: 4293 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3002 3047 4073 4432 4591 518 Size: 648 1836 45 1026 359 159 2042 Fragments arranged by size: 2042 1836 1026 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2153 3531 4208 5356 5601 1606 Size: 547 1378 677 1148 245 2120 Fragments arranged by size: 2120 1378 1148 677 547 245 NaeI GCC'GGC Cuts at: 3280 3280 Size: 6115 NarI GG'CG_CC Cuts at: 2087 2087 Size: 6115 NcoI C'CATG_G Cuts at: 513 1065 2091 513 Size: 552 1026 4537 Fragments arranged by size: 4537 1026 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NgoMIV G'CCGG_C Cuts at: 3278 3278 Size: 6115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 3473 5018 1132 Size: 97 2244 1545 2229 Fragments arranged by size: 2244 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6115 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6115 PfoI T'CCnGG_A Cuts at: 2394 2535 2394 Size: 141 5974 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4184 4216 5697 5729 4184 Size: 32 1481 32 4570 Fragments arranged by size: 4570 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2698 3253 2698 Size: 555 5560 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspOMI G'GGCC_C Cuts at: 2095 2095 Size: 6115 PstI C_TGCA'G Cuts at: 830 2856 3467 830 Size: 2026 611 3478 Fragments arranged by size: 3478 2026 611 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 SacII CC_GC'GG Cuts at: 2154 2154 Size: 6115 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SbfI CC_TGCA'GG Cuts at: 3467 3467 Size: 6115 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SfcI C'TryA_G Cuts at: 826 1045 2852 3463 4598 5279 5470 826 Size: 219 1807 611 1135 681 191 1471 Fragments arranged by size: 1807 1471 1135 681 611 219 191 SfoI GGC'GCC Cuts at: 2088 2088 Size: 6115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6115 SmaI CCC'GGG Cuts at: 1872 2050 2103 2194 3442 1872 Size: 178 53 91 1248 4545 Fragments arranged by size: 4545 1248 178 91 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 3170 3257 4219 5120 5382 820 Size: 197 926 1227 87 962 901 262 1553 Fragments arranged by size: 1553 1227 962 926 901 262 197 87 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 3473 1132 Size: 2341 3774 SrfI GCCC'GGGC Cuts at: 2194 2194 Size: 6115 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6115 StyI C'CwwG_G Cuts at: 513 1065 2091 3554 513 Size: 552 1026 1463 3074 Fragments arranged by size: 3074 1463 1026 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2040 2044 2668 4142 4159 4312 4497 Size: 1255 460 4 624 1474 17 153 185 Cuts at: 4497 4942 325 Size: 445 1498 Fragments arranged by size: 1498 1474 1255 624 460 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4361 6073 96 Size: 274 80 33 51 1443 2384 1712 138 Fragments arranged by size: 2384 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2239 2271 6075 6107 2239 Size: 32 3804 32 2247 Fragments arranged by size: 3804 2247 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 2048 2101 2192 3440 1870 Size: 178 53 91 1248 4545 Fragments arranged by size: 4545 1248 178 91 53 XmnI GAAnn'nnTTC Cuts at: 4244 4244 Size: 6115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI