Restriction map for
pFN21AB8199
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 103 CGCG CG^CG
AcsI 585 GAATTT [GA]^AATT[CT]
AlwI 345 GATCC GGATC(4/5)
ApoI 585 GAATTT [GA]^AATT[CT]
Asp700I 387 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 81 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 185 TGCGCA TGC^GCA
BfaI 479 CTAG C^TAG
BglI 65 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 573 AGATCT A^GATCT
BpmI 545 CTGGAG CTGGAG(16/14)
BsmBI 415 GAGACG CGTCTC(1/5)
BspHI 146 TCATGA T^CATGA
BsrBI 20 CCGCTC CCGCTC(-3/-3)
BstAPI 532 GCACCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 103 CGCG CG^CG
BstYI 573 AGATCT [GA]^GATC[CT]
EaeI 494 TGGCCG [CT]^GGCC[GA]
EarI 310 GAAGAG CTCTTC(1/4)
EcoT14I 333 CCAAGG C^C[AT][AT]GG
FauI 19 CCCGC CCCGC(4/6)
FspI 185 TGCGCA TGC^GCA
HgaI 81 GACGC GACGC(5/10)
HinfI 31 GACTC G^A[ACGT]TC
Ksp632I 310 GAAGAG CTCTTC(1/4)
MaeI 479 CTAG C^TAG
MflI 573 AGATCT [GA]^GATC[CT]
MspA1I 424 CAGCTG C[AC]G^C[GT]G
MvnI 103 CGCG CG^CG
PflFI 81 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 31 GACTC GAGTC(4/5)
PstI 172 CTGCAG CTGCA^G
PvuII 424 CAGCTG CAG^CTG
RcaI 146 TCATGA T^CATGA
SfcI 172 CTGCAG C^T[GA][CT]AG
SpeI 478 ACTAGT A^CTAGT
StyI 333 CCAAGG C^C[AT][AT]GG
TaqI 48 TCGA T^CGA
Tsp509I 586 AATT ^AATT
TspRI 341 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 81 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 48 TCGA T^CGA
XcmI 509 CCAAACAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 573 AGATCT [GA]^GATC[CT]
XmnI 387 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
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AvaI 35 CTCGGG C^[CT]CG[GA]G
113 CCCGAG
BsoBI 35 CTCGGG C^[CT]CG[GA]G
113 CCCGAG
BsrI 492 ACTGG ACTGG(1/-1)
544 ACTGG
BstF5I 522 GGATG GGATG(2/0)
552 GGATG
Cac8I 174 GCAGGC GC[ACGT]^[ACGT]GC
208 GCTCGC
CfoI 14 GCGC GCG^C
186 GCGC
DpnII 345 GATC ^GATC
574 GATC
FokI 522 GGATG GGATG(9/13)
552 GGATG
HaeIII 406 GGCC GG^CC
495 GGCC
HhaI 14 GCGC GCG^C
186 GCGC
HinP1I 14 GCGC G^CGC
186 GCGC
HphI 315 GGTGA GGTGA(8/7)
395 TCACC
MboI 345 GATC ^GATC
574 GATC
MboII 219 GAAGA GAAGA(8/7)
310 GAAGA
NdeII 345 GATC ^GATC
574 GATC
NlaIII 147 CATG CATG^
409 CATG
NlaIV 109 GGAACC GG[ACGT]^[ACGT]CC
123 GGAGCC
Sau3AI 345 GATC ^GATC
574 GATC
SfaNI 373 GCATC GCATC(5/9)
446 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmAI BssKI BstNI DdeI
EcoRII Fnu4HI ItaI MnlI MseI
MvaI MwoI ScrFI Tru9I TseI