Restriction map for
pFN21AB8197
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HapII Hin1I HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TfiI Tru9I TseI
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 34 GTCGAC GT^[AC][GT]AC
AccII 55 CGCG CG^CG
AciI 54 CCGC CCGC(-3/-1)
AluI 68 AGCT AG^CT
Aor51HI 428 AGCGCT AGC^GCT
Asp700I 100 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 79 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 43 GAGCCC G[GA]GC[CT]^C
BglI 206 GCCATAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 122 CTGGAG CTGGAG(16/14)
BsgI 342 CTGCAC GTGCAG(16/14)
BsiYI 230 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 230 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 58 GTCTC GTCTC(1/5)
BsmBI 57 CGTCTC CGTCTC(1/5)
BsmFI 85 GTCCC GGGAC(10/14)
BstUI 55 CGCG CG^CG
CfoI 429 GCGC GCG^C
DpnII 465 GATC ^GATC
DrdI 25 GACATTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 428 AGCGCT AGC^GCT
Fnu4HI 53 GCCGC GC^[ACGT]GC
HaeII 428 AGCGCT [GA]GCGC^[CT]
HgaI 56 GCGTC GACGC(5/10)
HhaI 429 GCGC GCG^C
HinP1I 429 GCGC G^CGC
ItaI 53 GCCGC GC^[ACGT]GC
MaeIII 194 GTGAC ^GT[ACGT]AC
MboI 465 GATC ^GATC
MnlI 418 GAGG CCTC(7/6)
MvnI 55 CGCG CG^CG
NdeII 465 GATC ^GATC
NspI 375 GCATGT [GA]CATG^[CT]
PflFI 79 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 34 GTCGAC G^TCGAC
Sau3AI 465 GATC ^GATC
SfaNI 287 GCATC GCATC(5/9)
SspI 409 AATATT AAT^ATT
Tsp45I 194 GTGAC ^GT[GC]AC
Tth111I 79 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 207 CCATAATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 100 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 102 AAATTT [GA]^AATT[CT]
244 AAATTT
ApoI 102 AAATTT [GA]^AATT[CT]
244 AAATTT
AspHI 110 GTGCTC G[AT]GC[AT]^C
117 GAGCAC
BsiHKAI 110 GTGCTC G[AT]GC[AT]^C
117 GAGCAC
BsrDI 27 CATTGC GCAATG(2/0)
471 CATTGC
BstF5I 264 CATCC GGATG(2/0)
366 GGATG
Cac8I 41 GCGAGC GC[ACGT]^[ACGT]GC
371 GCAAGC
DdeI 115 CTGAG C^T[ACGT]AG
356 CTGAG
DraII 83 AGGTCCC [GA]G^G[ACGT]CC[CT]
279 GGGTCCT
DsaI 296 CCATGG C^C[GA][CT]GG
422 CCATGG
EcoO109I 83 AGGTCCC [GA]G^G[ACGT]CC[CT]
279 GGGTCCT
FokI 264 CATCC GGATG(9/13)
366 GGATG
HaeIII 52 GGCC GG^CC
420 GGCC
HincII 4 GTCAAC GT[CT]^[GA]AC
34 GTCGAC
HindII 4 GTCAAC GT[CT]^[GA]AC
34 GTCGAC
HphI 193 GGTGA GGTGA(8/7)
467 TCACC
NcoI 296 CCATGG C^CATGG
422 CCATGG
PpuMI 83 AGGTCCC [GA]G^G[AT]CC[CT]
279 GGGTCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AvaII BmyI BsaJI Bsp1286I BsrI
BssKI BstNI Cfr13I EcoRII EcoT14I
MboII MvaI MwoI NlaIII NlaIV
Sau96I ScrFI StyI TaqI Tsp509I
TspRI TthHB8I