Restriction map for
pFN21AB8196
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaBI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstAPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EarI Eco47III EcoNI EcoRV EcoT22I
FbaI FseI FspI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 1054 TCCGGA T^CCGGA
AcsI 1044 GAATTC [GA]^AATT[CT]
AflIII 641 ACACGT A^C[GA][CT]GT
AocI 1360 CCTGAGG CC^T[ACGT]AGG
ApoI 1044 GAATTC [GA]^AATT[CT]
BalI 1241 TGGCCA TGG^CCA
BamHI 669 GGATCC G^GATCC
BanI 506 GGCACC G^G[CT][GA]CC
BbvCI 693 GCTGAGG CCTCAGC(-5/-2)
BfaI 407 CTAG C^TAG
BsaAI 642 CACGTA [CT]AC^GT[GA]
BsaWI 1054 TCCGGA [AT]^CCGG[AT]
BseAI 1054 TCCGGA T^CCGGA
BsiEI 386 CGGCCG CG[GA][CT]^CG
BsmI 65 GCATTC GAATGC(1/-1)
BspEI 1054 TCCGGA T^CCGGA
BspMI 474 ACCTGC ACCTGC(4/8)
BsrFI 503 ACCGGC [GA]^CCGG[CT]
BssHII 309 GCGCGC G^CGCGC
BstBI 1292 TTCGAA TT^CGAA
BstEII 255 GGTGACC G^GT[ACGT]ACC
BstPI 255 GGTGACC G^GT[ACGT]ACC
BstXI 845 CCAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1360 CCTGAGG CC^T[ACGT]AGG
Cfr10I 503 ACCGGC [GA]^CCGG[CT]
EagI 386 CGGCCG C^GGCCG
EclXI 386 CGGCCG C^GGCCG
Eco52I 386 CGGCCG C^GGCCG
Eco81I 1360 CCTGAGG CC^T[ACGT]AGG
EcoO65I 255 GGTGACC G^GT[ACGT]ACC
EcoRI 1044 GAATTC G^AATTC
EcoT14I 776 CCATGG C^C[AT][AT]GG
HincII 931 GTTAAC GT[CT]^[GA]AC
HindII 931 GTTAAC GT[CT]^[GA]AC
HindIII 1146 AAGCTT A^AGCTT
HpaI 931 GTTAAC GTT^AAC
MaeI 407 CTAG C^TAG
MluNI 1241 TGGCCA TGG^CCA
MroI 1054 TCCGGA T^CCGGA
MscI 1241 TGGCCA TGG^CCA
MseI 932 TTAA T^TAA
NcoI 776 CCATGG C^CATGG
NspI 208 GCATGC [GA]CATG^[CT]
NspV 1292 TTCGAA TT^CGAA
PstI 796 CTGCAG CTGCA^G
SfuI 1292 TTCGAA TT^CGAA
SphI 208 GCATGC GCATG^C
StuI 281 AGGCCT AGG^CCT
StyI 776 CCATGG C^C[AT][AT]GG
Tru9I 932 TTAA T^TAA
Tsp509I 1045 AATT ^AATT
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 117 GACGTC GACGT^C
216 GACGTC
AcyI 117 GACGTC G[GA]^CG[CT]C
216 GACGTC
AlwNI 165 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
470 CAGCACCTG
BbsI 465 GAAGAC GAAGAC(2/6)
1010 GTCTTC
BpmI 33 CTGGAG CTGGAG(16/14)
917 CTGGAG
BpuAI 465 GAAGAC GAAGAC(2/6)
1010 GTCTTC
BsaHI 117 GACGTC G[GA]^CG[CT]C
216 GACGTC
BsaI 81 GAGACC GGTCTC(1/5)
848 GAGACC
BseRI 1050 CTCCTC GAGGAG(10/8)
78 GAGGAG
BsrI 164 CCAGT ACTGG(1/-1)
1305 CCAGT
EaeI 386 CGGCCG [CT]^GGCC[GA]
1241 TGGCCA
HgaI 737 GACGC GACGC(5/10)
837 GCGTC
Hin1I 117 GACGTC G[GA]^CG[CT]C
216 GACGTC
MslI 660 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1083 CATTGGGGTG
PflMI 164 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCATGTGGTGG
SfcI 796 CTGCAG C^T[GA][CT]AG
1129 CTACAG
Van91I 164 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCATGTGGTGG
XcmI 584 CCAACGCCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
722 CCACGCTGCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AhdI AluI
AlwI ApaI Asp700I AspEI AvaI
AvaII BanII BbvI BcnI BlpI
BmyI Bpu1102I BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI Eam1105I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI XmnI