(Circular) (Six-base) MAPSORT of: pFN21AB8196.seq Check: 999 from: 1 to: 6079 pFN21AB8196 6079 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2488 2488 Size: 6079 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2126 2225 3889 278 Size: 53 83 186 936 590 99 1664 2468 Fragments arranged by size: 2468 1664 936 590 186 99 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3297 4142 5526 5794 1632 Size: 1665 845 1384 268 1917 Fragments arranged by size: 1917 1665 1384 845 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 2646 4978 5955 1203 Size: 1443 2332 977 1327 Fragments arranged by size: 2332 1443 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 2169 2235 3111 4808 2169 Size: 66 876 1697 3440 Fragments arranged by size: 3440 1697 876 66 AleI CACnn'nnGTG Cuts at: 1166 2669 1166 Size: 1503 4576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 2175 2480 3822 5394 2175 Size: 305 1342 1572 2860 Fragments arranged by size: 2860 1572 1342 305 ApaI G_GGCC'C Cuts at: 2116 2149 2182 2116 Size: 33 33 6013 Fragments arranged by size: 6013 33 33 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 3049 3388 3614 3766 5875 1493 Size: 1556 339 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 339 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2142 2148 2208 2233 2241 2280 3404 Size: 73 199 6 60 25 8 39 1124 Cuts at: 3404 1870 Size: 4545 Fragments arranged by size: 4545 1124 199 73 60 39 25 8 6 BamHI G'GATC_C Cuts at: 2674 3409 3776 2674 Size: 735 367 4977 Fragments arranged by size: 4977 735 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2511 3400 4855 618 Size: 325 217 627 724 889 1455 1842 Fragments arranged by size: 1842 1455 889 724 627 325 217 BanII G_rGCy'C Cuts at: 1391 2116 2149 2182 2580 3203 3398 1391 Size: 725 33 33 398 623 195 4072 Fragments arranged by size: 4072 725 623 398 195 33 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2477 3008 928 Size: 971 578 531 3999 Fragments arranged by size: 3999 971 578 531 BbvCI CC'TCA_GC Cuts at: 2699 2699 Size: 6079 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4690 136 Size: 107 122 71 631 605 3018 1525 Fragments arranged by size: 3018 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 2636 2746 2838 3496 2636 Size: 110 92 658 5219 Fragments arranged by size: 5219 658 110 92 Bme1580I G_kGCm'C Cuts at: 1790 2116 2149 2182 2248 4140 5296 1790 Size: 326 33 33 66 1892 1156 2573 Fragments arranged by size: 2573 1892 1156 326 66 33 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2163 3304 3810 Size: 416 53 83 399 24 746 1141 506 Cuts at: 3810 4768 442 Size: 958 1753 Fragments arranged by size: 1753 1141 958 746 506 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2943 4739 1239 Size: 820 884 1796 2579 Fragments arranged by size: 2579 1796 884 820 Bpu10I CC'TnA_GC Cuts at: 2551 2699 2551 Size: 148 5931 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4204 5069 5367 4204 Size: 865 298 4916 Fragments arranged by size: 4916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2080 2847 4742 882 Size: 617 581 767 1895 2219 Fragments arranged by size: 2219 1895 767 617 581 BsaAI yAC'GTr Cuts at: 493 1204 2649 3870 493 Size: 711 1445 1221 2702 Fragments arranged by size: 2702 1445 1221 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2123 2222 3886 4268 Size: 53 83 186 936 590 99 1664 382 Cuts at: 4268 4903 275 Size: 635 1451 Fragments arranged by size: 1664 1451 936 635 590 382 186 99 83 53 BsaWI w'CCGG_w Cuts at: 3059 4512 4910 5184 5331 5742 3059 Size: 1453 398 274 147 411 3396 Fragments arranged by size: 3396 1453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2077 2107 1178 Size: 30 165 30 674 30 5150 Fragments arranged by size: 5150 674 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 3046 1186 Size: 912 948 4219 Fragments arranged by size: 4219 948 912 BseYI C'CCAG_C Cuts at: 2076 2335 2342 3075 3116 5282 5778 2076 Size: 259 7 733 41 2166 496 2377 Fragments arranged by size: 2377 2166 733 496 259 41 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2052 3192 3341 1163 Size: 573 316 1140 149 3901 Fragments arranged by size: 3901 1140 573 316 149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2394 3529 4290 5318 1540 Size: 275 186 393 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 393 275 186 BsiHKAI G_wGCw'C Cuts at: 4140 4225 5296 4140 Size: 85 1071 4923 Fragments arranged by size: 4923 1071 85 BsmI GAATG_Cn' Cuts at: 2070 3590 3683 2070 Size: 1520 93 4466 Fragments arranged by size: 4466 1520 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2149 2182 2248 2580 3203 3398 Size: 399 326 33 33 66 332 623 195 Cuts at: 3398 4140 4225 5296 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 623 399 332 326 195 85 66 33 33 BspEI T'CCGG_A Cuts at: 3059 5742 3059 Size: 2683 3396 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 3434 844 Size: 1644 946 3489 Fragments arranged by size: 3489 1644 946 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2508 4723 4910 1700 Size: 110 698 2215 187 2869 Fragments arranged by size: 2869 2215 698 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 2314 1925 Size: 389 5690 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 3298 4883 3298 Size: 1585 4494 BstEII G'GTnAC_C Cuts at: 1184 2260 1184 Size: 1076 5003 BstXI CCAn_nnnn'nTGG Cuts at: 2857 2857 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2499 2674 3324 3409 3776 4161 4178 5630 Size: 527 175 650 85 367 385 17 1452 Cuts at: 5630 5716 5728 6074 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 650 527 385 367 346 175 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 3366 3366 Size: 6079 BtgI C'CryG_G Cuts at: 513 1065 2004 2484 2658 2679 2781 513 Size: 552 939 480 174 21 102 3811 Fragments arranged by size: 3811 939 552 480 174 102 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2437 2674 487 Size: 683 56 1211 237 3892 Fragments arranged by size: 3892 1211 683 237 56 BtsI GCAGTG_nn' Cuts at: 2358 2798 3324 3595 4389 4409 2358 Size: 440 526 271 794 20 4028 Fragments arranged by size: 4028 794 526 440 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 3246 3526 Size: 54 1425 50 275 18 561 855 280 Cuts at: 3526 4415 6044 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 855 561 280 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2391 3526 1537 Size: 275 579 1135 4090 Fragments arranged by size: 4090 1135 579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4009 1353 Size: 2656 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2943 3297 4142 4739 5526 5794 Size: 393 427 884 354 845 597 787 268 Cuts at: 5794 1239 Size: 1524 Fragments arranged by size: 1524 884 845 787 597 427 393 354 268 EcoO109I rG'GnC_Cy Cuts at: 2113 2163 2229 2237 2277 2384 2533 2927 3072 Size: 50 66 8 40 107 149 394 145 Cuts at: 3072 2113 Size: 5120 Fragments arranged by size: 5120 394 149 145 107 66 50 40 8 EcoRI G'AATT_C Cuts at: 3049 3388 3766 3049 Size: 339 378 5362 Fragments arranged by size: 5362 378 339 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 2336 2343 3085 5226 1678 Size: 658 7 742 2141 2531 Fragments arranged by size: 2531 2141 742 658 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2155 2187 2221 2253 3097 Size: 32 135 32 583 32 34 32 844 Cuts at: 3097 3129 4720 4752 4794 4826 1373 Size: 32 1591 32 42 32 2626 Fragments arranged by size: 2626 1591 844 583 135 42 34 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2938 3423 3669 669 Size: 988 281 1000 485 246 3079 Fragments arranged by size: 3079 1000 988 485 281 246 HindIII A'AGCT_T Cuts at: 748 3151 748 Size: 2403 3676 HpaI GTT'AAC Cuts at: 2938 3669 2938 Size: 731 5348 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5193 5377 1305 Size: 3888 184 2007 Fragments arranged by size: 3888 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3248 10 Size: 54 1425 343 1416 2841 Fragments arranged by size: 2841 1425 1416 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2669 3092 4037 4396 4555 518 Size: 648 1503 423 945 359 159 2042 Fragments arranged by size: 2042 1503 945 648 423 359 159 MspA1I CmG'CkG Cuts at: 1606 2669 2750 2984 3079 3495 4172 5320 5565 Size: 1063 81 234 95 416 677 1148 245 Cuts at: 5565 1606 Size: 2120 Fragments arranged by size: 2120 1148 1063 677 416 245 234 95 81 NcoI C'CATG_G Cuts at: 513 1065 2004 2781 513 Size: 552 939 777 3811 Fragments arranged by size: 3811 939 777 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 2217 3437 4982 1132 Size: 97 988 1220 1545 2229 Fragments arranged by size: 2229 1545 1220 988 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2175 2602 1108 Size: 1067 427 4585 Fragments arranged by size: 4585 1067 427 PfoI T'CCnGG_A Cuts at: 2502 2502 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2163 2229 2237 2277 2384 2533 2927 3072 2163 Size: 66 8 40 107 149 394 145 5170 Fragments arranged by size: 5170 394 149 145 107 66 40 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 2112 2145 2178 2112 Size: 33 33 6013 Fragments arranged by size: 6013 33 33 PstI C_TGCA'G Cuts at: 830 2805 3431 830 Size: 1975 626 3478 Fragments arranged by size: 3478 1975 626 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2669 2750 2984 2669 Size: 81 234 5764 Fragments arranged by size: 5764 234 81 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SanDI GG'GwC_CC Cuts at: 2163 2229 2237 3072 2163 Size: 66 8 835 5170 Fragments arranged by size: 5170 835 66 8 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 2801 3134 3427 4562 5243 5434 826 Size: 219 1756 333 293 1135 681 191 1471 Fragments arranged by size: 1756 1471 1135 681 333 293 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 2144 2235 2243 2282 3406 1872 Size: 272 91 8 39 1124 4545 Fragments arranged by size: 4545 1124 272 91 39 8 SmlI C'TyrA_G Cuts at: 820 1017 1943 4183 5084 5346 820 Size: 197 926 2240 901 262 1553 Fragments arranged by size: 2240 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 2217 3437 1132 Size: 1085 1220 3774 Fragments arranged by size: 3774 1220 1085 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 2288 1517 Size: 771 5308 StyI C'CwwG_G Cuts at: 513 1065 2004 2781 3518 513 Size: 552 939 777 737 3074 Fragments arranged by size: 3074 939 777 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 3175 4325 Size: 274 80 33 51 1443 465 733 1150 Cuts at: 4325 6037 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1150 733 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2547 2579 6039 6071 2547 Size: 32 3460 32 2555 Fragments arranged by size: 3460 2555 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2734 2596 Size: 138 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 2142 2233 2241 2280 3404 1870 Size: 272 91 8 39 1124 4545 Fragments arranged by size: 4545 1124 272 91 39 8 XmnI GAAnn'nnTTC Cuts at: 2308 2886 3294 4208 2308 Size: 578 408 914 4179 Fragments arranged by size: 4179 914 578 408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2124 2223 3887 276 Size: 53 83 186 936 590 99 1664 2468 Fragments arranged by size: 2468 1664 936 590 186 99 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI