Restriction map for
pFN21AB8196
(Linear) (Six-base) MAPSORT of: KIBB8196.seq Check: 4248 from: 1 to: 1374
KIBB8196.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 483 1374
Size: 483 891
AatII G_ACGT'C
Cuts at: 0 121 220 1374
Size: 121 99 1154
Fragments arranged by size:
1154 121 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1292 1374
Size: 1292 82
AflIII A'CryG_T
Cuts at: 0 641 1374
Size: 641 733
AhdI GACnn_n'nnGTC
Cuts at: 0 164 230 1106 1374
Size: 164 66 876 268
Fragments arranged by size:
876 268 164 66
AleI CACnn'nnGTG
Cuts at: 0 664 1374
Size: 664 710
AlwNI CAG_nnn'CTG
Cuts at: 0 170 475 1374
Size: 170 305 899
Fragments arranged by size:
899 305 170
ApaI G_GGCC'C
Cuts at: 0 111 144 177 1374
Size: 111 33 33 1197
Fragments arranged by size:
1197 111 33 33
ApoI r'AATT_y
Cuts at: 0 1044 1374
Size: 1044 330
AvaI C'yCGr_G
Cuts at: 0 137 143 203 228 236 275 1374
Size: 137 6 60 25 8 39 1099
Fragments arranged by size:
1099 137 60 39 25 8 6
BamHI G'GATC_C
Cuts at: 0 669 1374
Size: 669 705
BanI G'GyrC_C
Cuts at: 0 506 1374
Size: 506 868
BanII G_rGCy'C
Cuts at: 0 111 144 177 575 1198 1374
Size: 111 33 33 398 623 176
Fragments arranged by size:
623 398 176 111 33 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 472 1003 1374
Size: 472 531 371
Fragments arranged by size:
531 472 371
BbvCI CC'TCA_GC
Cuts at: 0 694 1374
Size: 694 680
BlpI GC'TnA_GC
Cuts at: 0 631 741 833 1374
Size: 631 110 92 541
Fragments arranged by size:
631 541 110 92
Bme1580I G_kGCm'C
Cuts at: 0 111 144 177 243 1374
Size: 111 33 33 66 1131
Fragments arranged by size:
1131 111 66 33 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 158 1299 1374
Size: 158 1141 75
Fragments arranged by size:
1141 158 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 938 1374
Size: 54 884 436
Fragments arranged by size:
884 436 54
Bpu10I CC'TnA_GC
Cuts at: 0 546 694 1374
Size: 546 148 680
Fragments arranged by size:
680 546 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 842 1374
Size: 75 767 532
Fragments arranged by size:
767 532 75
BsaAI yAC'GTr
Cuts at: 0 644 1374
Size: 644 730
BsaHI Gr'CG_yC
Cuts at: 0 118 217 1374
Size: 118 99 1157
Fragments arranged by size:
1157 118 99
BsaWI w'CCGG_w
Cuts at: 0 1054 1374
Size: 1054 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 72 102 1374
Size: 72 30 1272
Fragments arranged by size:
1272 72 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 1041 1374
Size: 93 948 333
Fragments arranged by size:
948 333 93
BseYI C'CCAG_C
Cuts at: 0 71 330 337 1070 1111 1374
Size: 71 259 7 733 41 263
Fragments arranged by size:
733 263 259 71 41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1187 1336 1374
Size: 47 1140 149 38
Fragments arranged by size:
1140 149 47 38
BsiEI CG_ry'CG
Cuts at: 0 389 1374
Size: 389 985
BsmI GAATG_Cn'
Cuts at: 0 65 1374
Size: 65 1309
Bsp1286I G_dGCh'C
Cuts at: 0 111 144 177 243 575 1198 1374
Size: 111 33 33 66 332 623 176
Fragments arranged by size:
623 332 176 111 66 33 33
BspEI T'CCGG_A
Cuts at: 0 1054 1374
Size: 1054 320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 1374
Size: 483 891
BsrFI r'CCGG_y
Cuts at: 0 503 1374
Size: 503 871
BssHII G'CGCG_C
Cuts at: 0 309 1374
Size: 309 1065
BstBI TT'CG_AA
Cuts at: 0 1293 1374
Size: 1293 81
BstEII G'GTnAC_C
Cuts at: 0 255 1374
Size: 255 1119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 852 1374
Size: 852 522
BstYI r'GATC_y
Cuts at: 0 494 669 1319 1374
Size: 494 175 650 55
Fragments arranged by size:
650 494 175 55
Bsu36I CC'TnA_GG
Cuts at: 0 1361 1374
Size: 1361 13
BtgI C'CryG_G
Cuts at: 0 479 653 674 776 1374
Size: 479 174 21 102 598
Fragments arranged by size:
598 479 174 102 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 432 669 1374
Size: 432 237 705
Fragments arranged by size:
705 432 237
BtsI GCAGTG_nn'
Cuts at: 0 353 793 1319 1374
Size: 353 440 526 55
Fragments arranged by size:
526 440 353 55
EaeI y'GGCC_r
Cuts at: 0 386 1241 1374
Size: 386 855 133
Fragments arranged by size:
855 386 133
EagI C'GGCC_G
Cuts at: 0 386 1374
Size: 386 988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 938 1292 1374
Size: 54 884 354 82
Fragments arranged by size:
884 354 82 54
EcoO109I rG'GnC_Cy
Cuts at: 0 108 158 224 232 272 379 528 922
Size: 108 50 66 8 40 107 149 394
Cuts at: 922 1067 1374
Size: 145 307
Fragments arranged by size:
394 307 149 145 108 107 66 50
40 8
EcoRI G'AATT_C
Cuts at: 0 1044 1374
Size: 1044 330
HaeII r_GCGC'y
Cuts at: 0 331 338 1080 1374
Size: 331 7 742 294
Fragments arranged by size:
742 331 294 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 216 248 1092 1124 1374
Size: 150 32 34 32 844 32 250
Fragments arranged by size:
844 250 150 34 32 32 32
HincII GTy'rAC
Cuts at: 0 933 1374
Size: 933 441
HindIII A'AGCT_T
Cuts at: 0 1146 1374
Size: 1146 228
HpaI GTT'AAC
Cuts at: 0 933 1374
Size: 933 441
MscI TGG'CCA
Cuts at: 0 1243 1374
Size: 1243 131
MslI CAynn'nnrTG
Cuts at: 0 664 1087 1374
Size: 664 423 287
Fragments arranged by size:
664 423 287
MspA1I CmG'CkG
Cuts at: 0 664 745 979 1074 1374
Size: 664 81 234 95 300
Fragments arranged by size:
664 300 234 95 81
NcoI C'CATG_G
Cuts at: 0 776 1374
Size: 776 598
NspI r_CATG'y
Cuts at: 0 212 1374
Size: 212 1162
PflMI CCAn_nnn'nTGG
Cuts at: 0 170 597 1374
Size: 170 427 777
Fragments arranged by size:
777 427 170
PfoI T'CCnGG_A
Cuts at: 0 497 1374
Size: 497 877
PpuMI rG'GwC_Cy
Cuts at: 0 158 224 232 272 379 528 922 1067
Size: 158 66 8 40 107 149 394 145
Cuts at: 1067 1374
Size: 307
Fragments arranged by size:
394 307 158 149 145 107 66 40
8
PspOMI G'GGCC_C
Cuts at: 0 107 140 173 1374
Size: 107 33 33 1201
Fragments arranged by size:
1201 107 33 33
PstI C_TGCA'G
Cuts at: 0 800 1374
Size: 800 574
PvuII CAG'CTG
Cuts at: 0 664 745 979 1374
Size: 664 81 234 395
Fragments arranged by size:
664 395 234 81
SanDI GG'GwC_CC
Cuts at: 0 158 224 232 1067 1374
Size: 158 66 8 835 307
Fragments arranged by size:
835 307 158 66 8
SfcI C'TryA_G
Cuts at: 0 796 1129 1374
Size: 796 333 245
Fragments arranged by size:
796 333 245
SmaI CCC'GGG
Cuts at: 0 139 230 238 277 1374
Size: 139 91 8 39 1097
Fragments arranged by size:
1097 139 91 39 8
SphI G_CATG'C
Cuts at: 0 212 1374
Size: 212 1162
StuI AGG'CCT
Cuts at: 0 283 1374
Size: 283 1091
StyI C'CwwG_G
Cuts at: 0 776 1374
Size: 776 598
TatI w'GTAC_w
Cuts at: 0 437 1170 1374
Size: 437 733 204
Fragments arranged by size:
733 437 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 542 574 1374
Size: 542 32 800
Fragments arranged by size:
800 542 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 729 1374
Size: 591 138 645
Fragments arranged by size:
645 591 138
XmaI C'CCGG_G
Cuts at: 0 137 228 236 275 1374
Size: 137 91 8 39 1099
Fragments arranged by size:
1099 137 91 39 8
XmnI GAAnn'nnTTC
Cuts at: 0 303 881 1289 1374
Size: 303 578 408 85
Fragments arranged by size:
578 408 303 85
ZraI GAC'GTC
Cuts at: 0 119 218 1374
Size: 119 99 1156
Fragments arranged by size:
1156 119 99
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspEI
BspMI BsrFI BssHII BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI Eco57MI EcoO109I EcoRI
HaeII Hin4I HincII HindIII HpaI MscI MslI MspA1I
NcoI NspI PflMI PfoI PpuMI PspOMI PstI PvuII
SanDI SfcI SmaI SphI StuI StyI TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
BpuEI BsaBI BsiHKAI BsiWI BsmBI BspHI BsrBI BsrDI
BsrGI BssSI BstAPI BstZ17I ClaI CspCI DraI DraIII
DrdI EarI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI TaqII Tth111I XbaI XhoI