(Circular) (Six-base) MAPSORT of: pFN21AB8195.seq Check: 3794 from: 1 to: 5644 pFN21AB8195 5644 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 3707 5091 5359 1632 Size: 1004 1071 1384 268 1917 Fragments arranged by size: 1917 1384 1071 1004 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2231 1184 Size: 1047 4597 AlwNI CAG_nnn'CTG Cuts at: 2047 2119 2176 2227 3387 4959 2047 Size: 72 57 51 1160 1572 2732 Fragments arranged by size: 2732 1572 1160 72 57 51 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2789 2965 4420 618 Size: 325 217 627 1002 176 1455 1842 Fragments arranged by size: 1842 1455 1002 627 325 217 176 BanII G_rGCy'C Cuts at: 1391 2237 2364 2963 1391 Size: 846 127 599 4072 Fragments arranged by size: 4072 846 599 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2154 928 Size: 971 255 4418 Fragments arranged by size: 4418 971 255 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 3705 4861 1790 Size: 1915 1156 2573 Fragments arranged by size: 2573 1915 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2281 2281 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2497 2582 4304 1239 Size: 902 356 85 1722 2579 Fragments arranged by size: 2579 1722 902 356 85 Bpu10I CC'TnA_GC Cuts at: 2088 2888 2088 Size: 800 4844 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2585 3769 4634 4932 2585 Size: 1184 865 298 3297 Fragments arranged by size: 3297 1184 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2530 3435 493 Size: 711 1326 905 2702 Fragments arranged by size: 2702 1326 905 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2912 3451 3833 4468 Size: 53 83 186 936 1379 539 382 635 Cuts at: 4468 275 Size: 1451 Fragments arranged by size: 1451 1379 936 635 539 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2901 4077 4475 4749 4896 5307 2901 Size: 1176 398 274 147 411 3238 Fragments arranged by size: 3238 1176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 1178 Size: 30 165 30 711 30 4678 Fragments arranged by size: 4678 711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2245 2273 1186 Size: 1059 28 4557 Fragments arranged by size: 4557 1059 28 BseYI C'CCAG_C Cuts at: 2368 2633 2847 2915 4847 5343 2368 Size: 265 214 68 1932 496 2669 Fragments arranged by size: 2669 1932 496 265 214 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 1163 Size: 573 723 4348 Fragments arranged by size: 4348 723 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 3705 3790 4861 2364 Size: 1341 85 1071 3147 Fragments arranged by size: 3147 1341 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2364 2963 3705 3790 4861 1391 Size: 399 447 127 599 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 599 447 399 127 85 BspEI T'CCGG_A Cuts at: 2901 5307 2901 Size: 2406 3238 BspHI T'CATG_A Cuts at: 2694 3533 5263 2694 Size: 839 1730 3075 Fragments arranged by size: 3075 1730 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2452 3531 1394 Size: 429 629 1079 3507 Fragments arranged by size: 3507 1079 629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2669 4288 4475 1700 Size: 110 859 1619 187 2869 Fragments arranged by size: 2869 1619 859 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 2708 4448 2708 Size: 1740 3904 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2898 2974 3341 3726 3743 5195 5281 5293 Size: 926 76 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 926 385 367 346 86 76 17 12 Bsu36I CC'TnA_GG Cuts at: 2228 2228 Size: 5644 BtgI C'CryG_G Cuts at: 513 1065 2004 2202 2538 2640 513 Size: 552 939 198 336 102 3517 Fragments arranged by size: 3517 939 552 336 198 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2650 487 Size: 683 56 1424 3481 Fragments arranged by size: 3481 1424 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2590 4651 809 Size: 507 1274 2061 1802 Fragments arranged by size: 2061 1802 1274 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2814 3091 3980 Size: 54 1425 50 275 18 984 277 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 984 889 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2497 2582 2636 3707 4304 5091 Size: 393 509 356 85 54 1071 597 787 Cuts at: 5091 5359 1239 Size: 268 1524 Fragments arranged by size: 1524 1071 787 597 509 393 356 268 85 54 EcoNI CCTnn'n_nnAGG Cuts at: 2491 2825 2491 Size: 334 5310 EcoO109I rG'GnC_Cy Cuts at: 2071 2224 2629 2071 Size: 153 405 5086 Fragments arranged by size: 5086 405 153 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2023 2284 2510 2922 4791 1678 Size: 345 261 226 412 1869 2531 Fragments arranged by size: 2531 1869 412 345 261 226 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2890 2922 4285 4317 4359 Size: 32 135 32 1318 32 1363 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1363 1318 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2619 2888 4758 4942 1305 Size: 1314 269 1870 184 2007 Fragments arranged by size: 2007 1870 1314 269 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2816 10 Size: 54 1425 343 984 2838 Fragments arranged by size: 2838 1425 984 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2699 3602 3961 4120 518 Size: 648 1533 903 359 159 2042 Fragments arranged by size: 2042 1533 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3060 3737 4885 5130 1606 Size: 1454 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 677 245 NaeI GCC'GGC Cuts at: 2671 2671 Size: 5644 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4153 Fragments arranged by size: 4153 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NgoMIV G'CCGG_C Cuts at: 2669 2669 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 1108 Size: 1011 4633 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PmlI CAC'GTG Cuts at: 2530 2530 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2224 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2996 830 Size: 2166 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SacI G_AGCT'C Cuts at: 2364 2364 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2407 2476 2992 4127 4808 4999 826 Size: 219 1362 69 516 1135 681 191 1471 Fragments arranged by size: 1471 1362 1135 681 516 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 3748 4649 4911 820 Size: 197 926 657 1148 901 262 1553 Fragments arranged by size: 1553 1148 926 901 657 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 2486 1517 Size: 969 4675 StyI C'CwwG_G Cuts at: 513 1065 2004 2775 3083 513 Size: 552 939 771 308 3074 Fragments arranged by size: 3074 939 771 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3671 3688 3841 4026 4471 325 Size: 1255 2091 17 153 185 445 1498 Fragments arranged by size: 2091 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3890 5602 96 Size: 274 80 33 51 1443 1913 1712 138 Fragments arranged by size: 1913 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2116 2647 2116 Size: 531 5113 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI