Restriction map for
pFN21AB8194
(Circular) (Six-base) MAPSORT of: pFN21AB8194.seq Check: 2635 from: 1 to: 6346
pFN21AB8194 6346 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4156 278
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 3689 1937
Size: 397 1355 4594
Fragments arranged by size:
4594 1355 397
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 2522 4473 4846 1563
Size: 959 1951 373 3063
Fragments arranged by size:
3063 1951 959 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4409 5793 6061 1632
Size: 2777 1384 268 1917
Fragments arranged by size:
2777 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6149
AflIII A'CryG_T
Cuts at: 1203 2706 5245 6222 1203
Size: 1503 2539 977 1327
Fragments arranged by size:
2539 1503 1327 977
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 5075 5075
Size: 6346
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6346
AlwNI CAG_nnn'CTG
Cuts at: 4089 5661 4089
Size: 1572 4774
ApaLI G'TGCA_C
Cuts at: 4403 5559 4403
Size: 1156 5190
ApoI r'AATT_y
Cuts at: 1493 2566 3234 3476 3655 3881 4033 6142 1493
Size: 1073 668 242 179 226 152 2109 1697
Fragments arranged by size:
2109 1697 1073 668 242 226 179 152
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 3084 3671 1870
Size: 73 1141 587 4545
Fragments arranged by size:
4545 1141 587 73
BamHI G'GATC_C
Cuts at: 3057 3466 3676 4043 3057
Size: 409 210 367 5360
Fragments arranged by size:
5360 409 367 210
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3578 3667 5122 618
Size: 325 217 627 1791 89 1455 1842
Fragments arranged by size:
1842 1791 1455 627 325 217 89
BanII G_rGCy'C
Cuts at: 1391 3665 1391
Size: 2274 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3330 928
Size: 971 1431 3944
Fragments arranged by size:
3944 1431 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4537 4571 1903
Size: 34 2600 34 3678
Fragments arranged by size:
3678 2600 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 5448 4238
Size: 1210 5136
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4957 136
Size: 107 122 71 631 605 3285 1525
Fragments arranged by size:
3285 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2026 6341 2026
Size: 4315 2031
BlpI GC'TnA_GC
Cuts at: 2040 3763 2040
Size: 1723 4623
Bme1580I G_kGCm'C
Cuts at: 1790 4407 5563 1790
Size: 2617 1156 2573
Fragments arranged by size:
2617 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 4077 5035
Size: 416 53 83 399 24 954 1706 958
Cuts at: 5035 442
Size: 1753
Fragments arranged by size:
1753 1706 958 954 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 3042 2419
Size: 623 5723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2753 5006 1239
Size: 1514 2253 2579
Fragments arranged by size:
2579 2253 1514
Bpu10I CC'TnA_GC
Cuts at: 3130 3130
Size: 6346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4471 5336 5634 4471
Size: 865 298 5183
Fragments arranged by size:
5183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4137 493
Size: 711 2933 2702
Fragments arranged by size:
2933 2702 711
BsaBI GATnn'nnATC
Cuts at: 6340 6340
Size: 6346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4153 4535 5170 275
Size: 53 83 186 936 2620 382 635 1451
Fragments arranged by size:
2620 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2834 4779 5177 5451 5598 6009 2834
Size: 1945 398 274 147 411 3171
Fragments arranged by size:
3171 1945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6121
Fragments arranged by size:
6121 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2190 1186
Size: 1004 5342
BseYI C'CCAG_C
Cuts at: 2163 3300 5549 6045 2163
Size: 1137 2249 496 2464
Fragments arranged by size:
2464 2249 1137 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2707 2765 1163
Size: 573 971 58 4744
Fragments arranged by size:
4744 971 573 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3796 4557 5585 1540
Size: 275 186 1795 761 1028 2301
Fragments arranged by size:
2301 1795 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4407 4492 5563 4407
Size: 85 1071 5190
Fragments arranged by size:
5190 1071 85
BsmI GAATG_Cn'
Cuts at: 3857 3950 3857
Size: 93 6253
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3665 4407 4492 5563 1391
Size: 399 1875 742 85 1071 2174
Fragments arranged by size:
2174 1875 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6009 6009
Size: 6346
BspHI T'CATG_A
Cuts at: 4235 5965 4235
Size: 1730 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2877 3701 844
Size: 2033 824 3489
Fragments arranged by size:
3489 2033 824
BsrBI CCG'CTC
Cuts at: 1394 1823 4233 1394
Size: 429 2410 3507
Fragments arranged by size:
3507 2410 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 4841 5015 65
Size: 1183 936 2657 174 1396
Fragments arranged by size:
2657 1396 1183 936 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4990 5177 1700
Size: 110 3180 187 2869
Fragments arranged by size:
3180 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6346
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 2093 3068 4406 5418 2093
Size: 975 1338 1012 3021
Fragments arranged by size:
3021 1338 1012 975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6346
BstBI TT'CG_AA
Cuts at: 2563 3393 5150 2563
Size: 830 1757 3759
Fragments arranged by size:
3759 1757 830
BstEII G'GTnAC_C
Cuts at: 1184 3146 1184
Size: 1962 4384
BstYI r'GATC_y
Cuts at: 1972 2026 3057 3466 3676 4043 4428 4445 5897
Size: 54 1031 409 210 367 385 17 1452
Cuts at: 5897 5983 5995 6341 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1031 409 385 367 346 210
86 54 17 12
BstZ17I GTA'TAC
Cuts at: 2335 2335
Size: 6346
BtgI C'CryG_G
Cuts at: 513 1065 3077 513
Size: 552 2012 3782
Fragments arranged by size:
3782 2012 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 2808 3862 4656 4676 2808
Size: 1054 794 20 4478
Fragments arranged by size:
4478 1054 794 20
ClaI AT'CG_AT
Cuts at: 1554 2112 1554
Size: 558 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 2842 3650 2842
Size: 808 5538
DraIII CAC_nnn'GTG
Cuts at: 2646 2794 2646
Size: 148 6198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5353 809
Size: 507 4037 1802
Fragments arranged by size:
4037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2823 3251 3793
Size: 54 1425 50 275 18 993 428 542
Cuts at: 3793 4682 6311 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 993 889 542 428 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3793 1537
Size: 275 1981 4090
Fragments arranged by size:
4090 1981 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4276 1353
Size: 2923 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4933 5307 5453 387
Size: 1064 138 3344 374 146 1280
Fragments arranged by size:
3344 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2753 4409 5006 5793 6061 1239
Size: 393 1121 1656 597 787 268 1524
Fragments arranged by size:
1656 1524 1121 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3655 4033 3655
Size: 378 5968
EcoRV GAT'ATC
Cuts at: 2182 3567 2182
Size: 1385 4961
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 5493 1678
Size: 3815 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2567 2599 4987 5019 5061
Size: 32 135 32 995 32 2388 32 42
Cuts at: 5061 5093 1373
Size: 32 2626
Fragments arranged by size:
2626 2388 995 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2712 3690 3936 669
Size: 988 281 774 978 246 3079
Fragments arranged by size:
3079 988 978 774 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6346
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 3945 3945
Size: 6346
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2622 3202 5460 5644 1305
Size: 1317 580 2258 184 2007
Fragments arranged by size:
2258 2007 1317 580 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2825 10
Size: 54 1425 343 993 3531
Fragments arranged by size:
3531 1425 993 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2700 3435 4304 4663 4822 518
Size: 648 1534 735 869 359 159 2042
Fragments arranged by size:
2042 1534 869 735 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2141 3762 4439 5587 5832 1606
Size: 535 1621 677 1148 245 2120
Fragments arranged by size:
2120 1621 1148 677 535 245
NcoI C'CATG_G
Cuts at: 513 1065 3077 513
Size: 552 2012 3782
Fragments arranged by size:
3782 2012 552
NdeI CA'TA_TG
Cuts at: 387 2856 387
Size: 2469 3877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6346
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 2710 3434 3502 3704 5249 1132
Size: 97 1481 724 68 202 1545 2229
Fragments arranged by size:
2229 1545 1481 724 202 97 68
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 2706 5245 2706
Size: 2539 3807
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6346
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PspXI vC'TCGA_Gb
Cuts at: 3084 3084
Size: 6346
PstI C_TGCA'G
Cuts at: 830 3698 830
Size: 2868 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 2141 2141
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 4594 6306 4594
Size: 1712 4634
SexAI A'CCwGG_T
Cuts at: 3036 3258 3036
Size: 222 6124
SfcI C'TryA_G
Cuts at: 826 1045 2058 2441 3607 3694 4829 5510 5701
Size: 219 1013 383 1166 87 1135 681 191
Cuts at: 5701 826
Size: 1471
Fragments arranged by size:
1471 1166 1135 1013 681 383 219 191
87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 3673 1872
Size: 1801 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3084 4450 5351 5613 820
Size: 197 926 1141 1366 901 262 1553
Fragments arranged by size:
1553 1366 1141 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3502 3704 1132
Size: 2370 202 3774
Fragments arranged by size:
3774 2370 202
SspI AAT'ATT
Cuts at: 5 52 2605 4270 5
Size: 47 2553 1665 2081
Fragments arranged by size:
2553 2081 1665 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6346
StyI C'CwwG_G
Cuts at: 513 1065 3077 3785 513
Size: 552 2012 708 3074
Fragments arranged by size:
3074 2012 708 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 2504 3389 4373 4390 4543 4728
Size: 1255 810 114 885 984 17 153 185
Cuts at: 4728 5173 325
Size: 445 1498
Fragments arranged by size:
1498 1255 984 885 810 445 185 153
114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3097 4592 6304
Size: 274 80 33 51 1443 1120 1495 1712
Cuts at: 6304 96
Size: 138
Fragments arranged by size:
1712 1495 1443 1120 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6306 6338 6306
Size: 32 6314
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6346
XbaI T'CTAG_A
Cuts at: 3682 3682
Size: 6346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2384 2384
Size: 6346
XhoI C'TCGA_G
Cuts at: 1943 3084 1943
Size: 1141 5205
XmaI C'CCGG_G
Cuts at: 1870 3671 1870
Size: 1801 4545
XmnI GAAnn'nnTTC
Cuts at: 4475 4475
Size: 6346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4154 276
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI