(Circular) (Six-base) MAPSORT of: pFN21AB8190.seq Check: 8671 from: 1 to: 5152 pFN21AB8190 5152 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2962 278 Size: 53 83 186 936 1426 2468 Fragments arranged by size: 2468 1426 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2046 2495 1937 Size: 109 449 4594 Fragments arranged by size: 4594 449 109 Acc65I G'GTAC_C Cuts at: 2473 2473 Size: 5152 AclI AA'CG_TT Cuts at: 1563 3279 3652 1563 Size: 1716 373 3063 Fragments arranged by size: 3063 1716 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3215 4599 4867 1632 Size: 1583 1384 268 1917 Fragments arranged by size: 1917 1583 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2158 820 Size: 197 1141 3814 Fragments arranged by size: 3814 1141 197 AflIII A'CryG_T Cuts at: 1203 4051 5028 1203 Size: 2848 977 1327 Fragments arranged by size: 2848 1327 977 AgeI A'CCGG_T Cuts at: 3983 3983 Size: 5152 AhdI GACnn_n'nnGTC Cuts at: 3881 3881 Size: 5152 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5152 AlwNI CAG_nnn'CTG Cuts at: 2895 4467 2895 Size: 1572 3580 ApaLI G'TGCA_C Cuts at: 3209 4365 3209 Size: 1156 3996 ApoI r'AATT_y Cuts at: 1493 2461 2687 2839 4948 1493 Size: 968 226 152 2109 1697 Fragments arranged by size: 2109 1697 968 226 152 AseI AT'TA_AT Cuts at: 160 1032 3706 160 Size: 872 2674 1606 Fragments arranged by size: 2674 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5152 AvaI C'yCGr_G Cuts at: 1870 1943 2477 1870 Size: 73 534 4545 Fragments arranged by size: 4545 534 73 BamHI G'GATC_C Cuts at: 2482 2849 2482 Size: 367 4785 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2473 3928 618 Size: 325 217 627 686 1455 1842 Fragments arranged by size: 1842 1455 686 627 325 217 BanII G_rGCy'C Cuts at: 1391 2471 1391 Size: 1080 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3343 3377 1903 Size: 34 1406 34 3678 Fragments arranged by size: 3678 1406 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3044 4254 3044 Size: 1210 3942 BclI T'GATC_A Cuts at: 1548 1548 Size: 5152 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3763 136 Size: 107 122 71 631 605 2091 1525 Fragments arranged by size: 2091 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5147 5147 Size: 5152 BlpI GC'TnA_GC Cuts at: 2569 2569 Size: 5152 Bme1580I G_kGCm'C Cuts at: 1790 3213 4369 1790 Size: 1423 1156 2573 Fragments arranged by size: 2573 1423 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2883 3841 442 Size: 416 53 83 399 24 1466 958 1753 Fragments arranged by size: 1753 1466 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5152 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2277 3812 1239 Size: 1038 1535 2579 Fragments arranged by size: 2579 1535 1038 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3277 4142 4440 3277 Size: 865 298 3989 Fragments arranged by size: 3989 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3815 882 Size: 617 2316 2219 Fragments arranged by size: 2316 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2943 493 Size: 711 1739 2702 Fragments arranged by size: 2702 1739 711 BsaBI GATnn'nnATC Cuts at: 2106 5146 2106 Size: 3040 2112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2959 3341 3976 275 Size: 53 83 186 936 1426 382 635 1451 Fragments arranged by size: 1451 1426 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3585 3983 4257 4404 4815 3585 Size: 398 274 147 411 3922 Fragments arranged by size: 3922 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4927 Fragments arranged by size: 4927 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2382 1186 Size: 1196 3956 BseYI C'CCAG_C Cuts at: 4355 4851 4355 Size: 496 4656 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4579 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2602 3363 4391 1540 Size: 275 186 601 761 1028 2301 Fragments arranged by size: 2301 1028 761 601 275 186 BsiHKAI G_wGCw'C Cuts at: 3213 3298 4369 3213 Size: 85 1071 3996 Fragments arranged by size: 3996 1071 85 BsmI GAATG_Cn' Cuts at: 2663 2756 2663 Size: 93 5059 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2471 3213 3298 4369 1391 Size: 399 681 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 681 399 85 BspEI T'CCGG_A Cuts at: 4815 4815 Size: 5152 BspHI T'CATG_A Cuts at: 3041 4771 3041 Size: 1730 3422 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2507 844 Size: 1663 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3039 1394 Size: 429 1216 3507 Fragments arranged by size: 3507 1216 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2379 3647 3821 65 Size: 1183 1131 1268 174 1396 Fragments arranged by size: 1396 1268 1183 1131 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3796 3983 1700 Size: 110 1986 187 2869 Fragments arranged by size: 2869 1986 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5152 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5152 BssSI C'ACGA_G Cuts at: 3212 4224 3212 Size: 1012 4140 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5152 BstBI TT'CG_AA Cuts at: 3956 3956 Size: 5152 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5152 BstYI r'GATC_y Cuts at: 1972 2107 2288 2482 2849 3234 3251 4703 4789 Size: 135 181 194 367 385 17 1452 86 Cuts at: 4789 4801 5147 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 194 181 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4600 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4413 Fragments arranged by size: 4413 683 56 BtsI GCAGTG_nn' Cuts at: 2668 3462 3482 2668 Size: 794 20 4338 Fragments arranged by size: 4338 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5152 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5117 DraI TTT'AAA Cuts at: 2456 2456 Size: 5152 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4159 809 Size: 507 2843 1802 Fragments arranged by size: 2843 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2599 3488 5117 Size: 54 1425 50 275 18 769 889 1629 Cuts at: 5117 8 Size: 43 Fragments arranged by size: 1629 1425 889 769 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2599 1537 Size: 275 787 4090 Fragments arranged by size: 4090 787 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2155 3082 1353 Size: 802 927 3423 Fragments arranged by size: 3423 927 802 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3739 4113 4259 387 Size: 1064 138 2150 374 146 1280 Fragments arranged by size: 2150 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 3215 3812 4599 4867 1239 Size: 393 645 938 597 787 268 1524 Fragments arranged by size: 1524 938 787 645 597 393 268 EcoRI G'AATT_C Cuts at: 2461 2839 2461 Size: 378 4774 FspI TGC'GCA Cuts at: 3658 3658 Size: 5152 HaeII r_GCGC'y Cuts at: 1678 4299 1678 Size: 2621 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 3793 3825 3867 Size: 32 135 32 701 32 1488 32 42 Cuts at: 3867 3899 1373 Size: 32 2626 Fragments arranged by size: 2626 1488 701 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2496 2742 669 Size: 988 281 558 246 3079 Fragments arranged by size: 3079 988 558 281 246 HindIII A'AGCT_T Cuts at: 748 2317 2384 748 Size: 1569 67 3516 Fragments arranged by size: 3516 1569 67 HpaI GTT'AAC Cuts at: 2742 2742 Size: 5152 KpnI G_GTAC'C Cuts at: 2477 2477 Size: 5152 MfeI C'AATT_G Cuts at: 2751 2751 Size: 5152 MluI A'CGCG_T Cuts at: 5028 5028 Size: 5152 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4266 4450 1305 Size: 2961 184 2007 Fragments arranged by size: 2961 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3330 Fragments arranged by size: 3330 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3110 3469 3628 518 Size: 648 1944 359 159 2042 Fragments arranged by size: 2042 1944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2568 3245 4393 4638 1606 Size: 962 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4600 NdeI CA'TA_TG Cuts at: 387 2271 387 Size: 1884 3268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5152 NotI GC'GGCC_GC Cuts at: 2599 2599 Size: 5152 NspI r_CATG'y Cuts at: 1132 1229 2510 4055 1132 Size: 97 1281 1545 2229 Fragments arranged by size: 2229 1545 1281 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5152 PciI A'CATG_T Cuts at: 4051 4051 Size: 5152 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5152 PmeI GTTT'AAAC Cuts at: 2456 2456 Size: 5152 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3221 3253 4734 4766 3221 Size: 32 1481 32 3607 Fragments arranged by size: 3607 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5152 PsiI TTA'TAA Cuts at: 2722 2722 Size: 5152 PstI C_TGCA'G Cuts at: 830 2124 2206 2504 830 Size: 1294 82 298 3478 Fragments arranged by size: 3478 1294 298 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5152 SalI G'TCGA_C Cuts at: 1936 2494 1936 Size: 558 4594 SbfI CC_TGCA'GG Cuts at: 2504 2504 Size: 5152 ScaI AGT'ACT Cuts at: 2065 3400 5112 2065 Size: 1335 1712 2105 Fragments arranged by size: 2105 1712 1335 SexAI A'CCwGG_T Cuts at: 2181 2181 Size: 5152 SfcI C'TryA_G Cuts at: 826 1045 2120 2202 2500 3635 4316 4507 826 Size: 219 1075 82 298 1135 681 191 1471 Fragments arranged by size: 1471 1135 1075 681 298 219 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5152 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5152 SmaI CCC'GGG Cuts at: 1872 2479 1872 Size: 607 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2158 3256 4157 4419 820 Size: 197 926 215 1098 901 262 1553 Fragments arranged by size: 1553 1098 926 901 262 215 197 SnaBI TAC'GTA Cuts at: 493 2943 493 Size: 2450 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5152 SphI G_CATG'C Cuts at: 1132 2510 1132 Size: 1378 3774 SspI AAT'ATT Cuts at: 5 52 2419 3076 5 Size: 47 2367 657 2081 Fragments arranged by size: 2367 2081 657 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5152 StyI C'CwwG_G Cuts at: 513 1065 2591 513 Size: 552 1526 3074 Fragments arranged by size: 3074 1526 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3179 3196 3349 3534 3979 325 Size: 1255 1599 17 153 185 445 1498 Fragments arranged by size: 1599 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2063 3398 5110 Size: 274 80 33 51 1443 86 1335 1712 Cuts at: 5110 96 Size: 138 Fragments arranged by size: 1712 1443 1335 274 138 86 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5112 5144 5112 Size: 32 5120 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5152 XbaI T'CTAG_A Cuts at: 2488 2488 Size: 5152 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5152 XmaI C'CCGG_G Cuts at: 1870 2477 1870 Size: 607 4545 XmnI GAAnn'nnTTC Cuts at: 3281 3281 Size: 5152 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2960 276 Size: 53 83 186 936 1426 2468 Fragments arranged by size: 2468 1426 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI