(Circular) (Six-base) MAPSORT of: pFN21AB8189.seq Check: 1381 from: 1 to: 6043 pFN21AB8189 6043 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 1937 Size: 1449 4594 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 2625 2698 2782 2866 2950 3034 3202 Size: 702 291 73 84 84 84 84 168 Cuts at: 3202 4106 5490 5758 1632 Size: 904 1384 268 1917 Fragments arranged by size: 1917 1384 904 702 291 268 168 84 84 84 84 73 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 2925 1166 Size: 1759 4284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 2047 2152 3786 5358 2047 Size: 105 1634 1572 2732 Fragments arranged by size: 2732 1634 1572 105 ApaLI G'TGCA_C Cuts at: 4100 5256 4100 Size: 1156 4887 ApoI r'AATT_y Cuts at: 1493 2307 2554 3252 3352 3578 3730 5839 1493 Size: 814 247 698 100 226 152 2109 1697 Fragments arranged by size: 2109 1697 814 698 247 226 152 100 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 3368 1870 Size: 73 1425 4545 Fragments arranged by size: 4545 1425 73 BamHI G'GATC_C Cuts at: 2832 3373 3740 2832 Size: 541 367 5135 Fragments arranged by size: 5135 541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3364 4819 618 Size: 325 217 627 1577 1455 1842 Fragments arranged by size: 1842 1577 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2726 2894 2978 3062 3362 1391 Size: 1335 168 84 84 300 4072 Fragments arranged by size: 4072 1335 300 168 84 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 2882 928 Size: 971 423 560 4089 Fragments arranged by size: 4089 971 560 423 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4234 4268 1903 Size: 34 2297 34 3678 Fragments arranged by size: 3678 2297 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3935 5145 3935 Size: 1210 4833 BclI T'GATC_A Cuts at: 1548 2299 1548 Size: 751 5292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4654 136 Size: 107 122 71 631 605 2982 1525 Fragments arranged by size: 2982 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 3460 3460 Size: 6043 Bme1580I G_kGCm'C Cuts at: 1790 4104 5260 1790 Size: 2314 1156 2573 Fragments arranged by size: 2573 2314 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2351 2682 2766 Size: 416 53 83 399 24 934 331 84 Cuts at: 2766 3774 4732 442 Size: 1008 958 1753 Fragments arranged by size: 1753 1008 958 934 416 399 331 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2457 2908 3030 3114 3282 4703 1239 Size: 1028 190 451 122 84 168 1421 2579 Fragments arranged by size: 2579 1421 1028 451 190 168 122 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 4168 5033 5331 2360 Size: 1808 865 298 3072 Fragments arranged by size: 3072 1808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2276 2378 4706 882 Size: 617 777 102 2328 2219 Fragments arranged by size: 2328 2219 777 617 102 BsaAI yAC'GTr Cuts at: 493 1204 3834 493 Size: 711 2630 2702 Fragments arranged by size: 2702 2630 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3850 4232 4867 275 Size: 53 83 186 936 2317 382 635 1451 Fragments arranged by size: 2317 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2187 4476 4874 5148 5295 5706 2187 Size: 2289 398 274 147 411 2524 Fragments arranged by size: 2524 2289 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5818 Fragments arranged by size: 5818 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2501 1186 Size: 1315 4728 BseYI C'CCAG_C Cuts at: 2422 5246 5742 2422 Size: 2824 496 2723 Fragments arranged by size: 2824 2723 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5470 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3318 3493 4254 5282 1540 Size: 275 186 1317 175 761 1028 2301 Fragments arranged by size: 2301 1317 1028 761 275 186 175 BsiHKAI G_wGCw'C Cuts at: 2062 2726 2894 2978 3062 4104 4189 5260 2062 Size: 664 168 84 84 1042 85 1071 2845 Fragments arranged by size: 2845 1071 1042 664 168 85 84 84 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2726 2894 2978 3062 3362 4104 Size: 399 272 664 168 84 84 300 742 Cuts at: 4104 4189 5260 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 399 300 272 168 85 84 84 BspEI T'CCGG_A Cuts at: 2187 5706 2187 Size: 3519 2524 BspHI T'CATG_A Cuts at: 3932 5662 3932 Size: 1730 4313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2221 3398 844 Size: 1377 1177 3489 Fragments arranged by size: 3489 1377 1177 BsrBI CCG'CTC Cuts at: 1394 1823 3930 1394 Size: 429 2107 3507 Fragments arranged by size: 3507 2107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2953 4538 4712 65 Size: 1183 1705 1585 174 1396 Fragments arranged by size: 1705 1585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 4874 1700 Size: 110 2877 187 2869 Fragments arranged by size: 2877 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 2155 1184 Size: 971 5072 BstYI r'GATC_y Cuts at: 1972 2832 3373 3740 4125 4142 5594 5680 5692 Size: 860 541 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 860 541 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2040 Size: 6043 BtgI C'CryG_G Cuts at: 513 1065 2004 2586 3339 513 Size: 552 939 582 753 3217 Fragments arranged by size: 3217 939 753 582 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5304 Fragments arranged by size: 5304 683 56 BtsI GCAGTG_nn' Cuts at: 3559 4353 4373 3559 Size: 794 20 5229 Fragments arranged by size: 5229 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2935 2970 529 Size: 35 2371 35 3602 Fragments arranged by size: 3602 2371 35 35 DraI TTT'AAA Cuts at: 2383 3347 2383 Size: 964 5079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3490 4379 6008 Size: 54 1425 50 275 18 1660 889 1629 Cuts at: 6008 8 Size: 43 Fragments arranged by size: 1660 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3490 1537 Size: 275 1678 4090 Fragments arranged by size: 4090 1678 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 3973 1353 Size: 714 1906 3423 Fragments arranged by size: 3423 1906 714 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2724 2892 2976 3060 2724 Size: 168 84 84 5707 Fragments arranged by size: 5707 168 84 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2334 2457 2625 2698 2782 2866 Size: 393 635 67 123 168 73 84 84 Cuts at: 2866 2908 2950 3030 3034 3114 3202 3282 4106 Size: 42 42 80 4 80 88 80 824 Cuts at: 4106 4703 5490 5758 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 824 787 635 597 393 268 168 123 88 84 84 80 80 80 73 67 42 42 4 EcoNI CCTnn'n_nnAGG Cuts at: 2968 2968 Size: 6043 EcoO109I rG'GnC_Cy Cuts at: 2044 2224 2335 2044 Size: 180 111 5752 Fragments arranged by size: 5752 180 111 EcoRI G'AATT_C Cuts at: 2554 3252 3352 3730 2554 Size: 698 100 378 4867 Fragments arranged by size: 4867 698 378 100 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 5190 1678 Size: 3512 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2309 2341 2617 Size: 32 135 32 440 32 265 32 276 Cuts at: 2617 2649 4684 4716 4758 4790 1373 Size: 32 2035 32 42 32 2626 Fragments arranged by size: 2626 2035 440 276 265 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2112 2583 5157 5341 1305 Size: 807 471 2574 184 2007 Fragments arranged by size: 2574 2007 807 471 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4221 Fragments arranged by size: 4221 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2607 2925 4001 4360 4519 518 Size: 648 1441 318 1076 359 159 2042 Fragments arranged by size: 2042 1441 1076 648 359 318 159 MspA1I CmG'CkG Cuts at: 1606 2422 2802 2886 3459 4136 5284 5529 1606 Size: 816 380 84 573 677 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 573 380 245 84 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4552 Fragments arranged by size: 4552 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 3401 4946 1132 Size: 97 2172 1545 2229 Fragments arranged by size: 2229 2172 1545 97 PasI CC'CwG_GG Cuts at: 1348 2502 1348 Size: 1154 4889 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 2468 1108 Size: 1044 316 4683 Fragments arranged by size: 4683 1044 316 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2335 2224 Size: 111 5932 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PstI C_TGCA'G Cuts at: 830 3395 830 Size: 2565 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2802 2886 2802 Size: 84 5959 SacI G_AGCT'C Cuts at: 2726 2894 2978 3062 2726 Size: 168 84 84 5707 Fragments arranged by size: 5707 168 84 84 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SapI GCTCTTCn'nnn_ Cuts at: 2067 2067 Size: 6043 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SfcI C'TryA_G Cuts at: 826 1045 2230 2803 3139 3391 4526 5207 5398 Size: 219 1185 573 336 252 1135 681 191 Cuts at: 5398 826 Size: 1471 Fragments arranged by size: 1471 1185 1135 681 573 336 252 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3370 1872 Size: 1498 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2375 4147 5048 5310 820 Size: 197 926 432 1772 901 262 1553 Fragments arranged by size: 1772 1553 926 901 432 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 2711 2963 3150 1517 Size: 1194 252 187 4410 Fragments arranged by size: 4410 1194 252 187 StyI C'CwwG_G Cuts at: 513 1065 2004 3482 513 Size: 552 939 1478 3074 Fragments arranged by size: 3074 1478 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2918 3332 4070 4087 4240 4425 4870 Size: 1255 1338 414 738 17 153 185 445 Cuts at: 4870 325 Size: 1498 Fragments arranged by size: 1498 1338 1255 738 445 414 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 4289 6001 Size: 274 80 33 51 1443 201 2111 1712 Cuts at: 6001 96 Size: 138 Fragments arranged by size: 2111 1712 1443 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 6003 6035 2319 Size: 32 3652 32 2327 Fragments arranged by size: 3652 2327 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3368 1870 Size: 1498 4545 XmnI GAAnn'nnTTC Cuts at: 2264 2543 2711 2795 2879 2963 3047 3215 4172 Size: 279 168 84 84 84 84 168 957 Cuts at: 4172 2264 Size: 4135 Fragments arranged by size: 4135 957 279 168 168 84 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI