Restriction map for
pFN21AB8186
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I MaeIII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 7 CGCG CG^CG
AgeI 82 ACCGGT A^CCGGT
Alw44I 636 GTGCAC G^TGCAC
AlwNI 766 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 636 GTGCAC G^TGCAC
Asp700I 36 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 636 GTGCAC G[AT]GC[AT]^C
BbsI 252 GAAGAC GAAGAC(2/6)
BfaI 550 CTAG C^TAG
BglI 134 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 252 GAAGAC GAAGAC(2/6)
BsaAI 115 TACGTA [CT]AC^GT[GA]
BsaWI 82 ACCGGT [AT]^CCGG[AT]
BsiHKAI 636 GTGCAC G[AT]GC[AT]^C
BsiWI 833 CGTACG C^GTACG
BsmFI 876 GGGAC GGGAC(10/14)
BspHI 420 TCATGA T^CATGA
BspMI 761 ACCTGC ACCTGC(4/8)
BsrFI 82 ACCGGT [GA]^CCGG[CT]
BstUI 7 CGCG CG^CG
Cfr10I 82 ACCGGT [GA]^CCGG[CT]
EaeI 776 TGGCCG [CT]^GGCC[GA]
HapII 83 CCGG C^CGG
HindIII 454 AAGCTT A^AGCTT
HpaII 83 CCGG C^CGG
KspI 6 CCGCGG CCGC^GG
MaeI 550 CTAG C^TAG
MslI 159 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 83 CCGG C^CGG
MvnI 7 CGCG CG^CG
NcoI 800 CCATGG C^CATGG
NdeI 299 CATATG CA^TATG
PinAI 82 ACCGGT A^CCGGT
PleI 580 GAGTC GAGTC(4/5)
PvuII 30 CAGCTG CAG^CTG
RcaI 420 TCATGA T^CATGA
SacII 6 CCGCGG CCGC^GG
SnaBI 115 TACGTA TAC^GTA
SspI 120 AATATT AAT^ATT
XcmI 791 CCAATCTTACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 36 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 80 GGACC G^G[AT]CC
207 GGACC
BcgI 436 CGAAAGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
143 GCATCTTCATCG
BmyI 385 GTGCCC G[AGT]GC[ACT]^C
636 GTGCAC
BsiYI 730 CCGTACTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
867 CCGTTTTCAGG
BslI 730 CCGTACTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
867 CCGTTTTCAGG
BsmAI 410 GAGAC GTCTC(1/5)
589 GAGAC
Bsp1286I 385 GTGCCC G[AGT]GC[ACT]^C
636 GTGCAC
BstAPI 515 GCAAACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
765 GCAGCCCCTGC
BstF5I 159 CATCC GGATG(2/0)
354 GGATG
BstYI 101 AGATCC [GA]^GATC[CT]
245 AGATCC
DsaI 6 CCGCGG C^C[GA][CT]GG
800 CCATGG
FokI 159 CATCC GGATG(9/13)
354 GGATG
MaeII 116 ACGT A^CGT
879 ACGT
MflI 101 AGATCC [GA]^GATC[CT]
245 AGATCC
MspA1I 6 CCGCGG C[AC]G^C[GT]G
30 CAGCTG
SfaNI 168 GATGC GCATC(5/9)
143 GCATC
XhoII 101 AGATCC [GA]^GATC[CT]
245 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI BseRI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
HaeIII HinfI HphI ItaI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI