(Circular) (Six-base) MAPSORT of: pFN21AB8186.seq Check: 279 from: 1 to: 5614 pFN21AB8186 5614 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2935 2935 Size: 5614 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3677 5061 5329 1632 Size: 2045 1384 268 1917 Fragments arranged by size: 2045 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 4513 5490 1203 Size: 3310 977 1327 Fragments arranged by size: 3310 1327 977 AgeI A'CCGG_T Cuts at: 2087 4445 2087 Size: 2358 3256 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2768 1184 Size: 1584 4030 AlwNI CAG_nnn'CTG Cuts at: 2776 3357 4929 2776 Size: 581 1572 3461 Fragments arranged by size: 3461 1572 581 ApaLI G'TGCA_C Cuts at: 2641 3671 4827 2641 Size: 1030 1156 3428 Fragments arranged by size: 3428 1156 1030 ApoI r'AATT_y Cuts at: 1493 2045 2192 2265 2817 2923 3149 3301 5410 Size: 552 147 73 552 106 226 152 2109 Cuts at: 5410 1493 Size: 1697 Fragments arranged by size: 2109 1697 552 552 226 152 147 106 73 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2939 1870 Size: 73 996 4545 Fragments arranged by size: 4545 996 73 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2935 4390 618 Size: 325 217 627 1148 1455 1842 Fragments arranged by size: 1842 1455 1148 627 325 217 BanII G_rGCy'C Cuts at: 1391 2933 1391 Size: 1542 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2264 928 Size: 971 365 4278 Fragments arranged by size: 4278 971 365 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2137 2171 2430 2464 3805 3839 1903 Size: 34 200 34 259 34 1341 34 3678 Fragments arranged by size: 3678 1341 259 200 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2145 4225 136 Size: 107 122 71 631 605 473 2080 1525 Fragments arranged by size: 2080 1525 631 605 473 122 107 71 BglII A'GATC_T Cuts at: 5609 5609 Size: 5614 BlpI GC'TnA_GC Cuts at: 3031 3031 Size: 5614 Bme1580I G_kGCm'C Cuts at: 1790 2394 2645 3675 4831 1790 Size: 604 251 1030 1156 2573 Fragments arranged by size: 2573 1156 1030 604 251 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2388 2597 2708 Size: 416 53 83 399 24 971 209 111 Cuts at: 2708 3345 4303 442 Size: 637 958 1753 Fragments arranged by size: 1753 971 958 637 416 399 209 111 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4274 1239 Size: 3035 2579 Bpu10I CC'TnA_GC Cuts at: 2568 2568 Size: 5614 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3739 4604 4902 3739 Size: 865 298 4451 Fragments arranged by size: 4451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4277 882 Size: 617 2778 2219 Fragments arranged by size: 2778 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2122 3405 493 Size: 711 918 1283 2702 Fragments arranged by size: 2702 1283 918 711 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3421 3803 4438 275 Size: 53 83 186 936 1888 382 635 1451 Fragments arranged by size: 1888 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2087 4047 4445 4719 4866 5277 2087 Size: 1960 398 274 147 411 2424 Fragments arranged by size: 2424 1960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2427 2448 1186 Size: 856 385 21 4352 Fragments arranged by size: 4352 856 385 21 BseYI C'CCAG_C Cuts at: 2470 4817 5313 2470 Size: 2347 496 2771 Fragments arranged by size: 2771 2347 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5041 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3064 3825 4853 1540 Size: 275 186 1063 761 1028 2301 Fragments arranged by size: 2301 1063 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2645 3675 3760 4831 2645 Size: 1030 85 1071 3428 Fragments arranged by size: 3428 1071 1030 85 BsiWI C'GTAC_G Cuts at: 2838 2838 Size: 5614 BsmI GAATG_Cn' Cuts at: 3125 3218 3125 Size: 93 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2645 2933 3675 3760 4831 1391 Size: 399 604 251 288 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 288 251 85 BspEI T'CCGG_A Cuts at: 5277 5277 Size: 5614 BspHI T'CATG_A Cuts at: 2425 3503 5233 2425 Size: 1078 1730 2806 Fragments arranged by size: 2806 1730 1078 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2775 2969 844 Size: 1931 194 3489 Fragments arranged by size: 3489 1931 194 BsrBI CCG'CTC Cuts at: 1394 1823 3501 1394 Size: 429 1678 3507 Fragments arranged by size: 3507 1678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4109 4283 65 Size: 1183 2861 174 1396 Fragments arranged by size: 2861 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2087 4258 4445 1700 Size: 110 277 2171 187 2869 Fragments arranged by size: 2869 2171 277 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5614 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 3674 4686 3674 Size: 1012 4602 BstAPI GCAn_nnn'nTGC Cuts at: 794 2526 2776 794 Size: 1732 250 3632 Fragments arranged by size: 3632 1732 250 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5614 BstYI r'GATC_y Cuts at: 1972 2106 2250 2944 3311 3696 3713 5165 5251 Size: 134 144 694 367 385 17 1452 86 Cuts at: 5251 5263 5609 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 694 385 367 346 144 134 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2011 2805 513 Size: 552 939 7 794 3322 Fragments arranged by size: 3322 939 794 552 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2400 487 Size: 683 56 1174 3701 Fragments arranged by size: 3701 1174 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3924 3944 3130 Size: 794 20 4800 Fragments arranged by size: 4800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4621 809 Size: 507 3305 1802 Fragments arranged by size: 3305 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2781 3061 3950 Size: 54 1425 50 275 18 951 280 889 Cuts at: 3950 5579 8 Size: 1629 43 Fragments arranged by size: 1629 1425 951 889 280 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3061 1537 Size: 275 1249 4090 Fragments arranged by size: 4090 1249 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3544 1353 Size: 2191 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4201 4575 4721 387 Size: 1064 138 2612 374 146 1280 Fragments arranged by size: 2612 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3677 4274 5061 5329 1239 Size: 393 2045 597 787 268 1524 Fragments arranged by size: 2045 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2923 3301 2923 Size: 378 5236 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 4761 1678 Size: 3083 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373 Size: 32 135 32 2683 32 42 32 2626 Fragments arranged by size: 2683 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 2459 748 Size: 1711 3903 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KpnI G_GTAC'C Cuts at: 2939 2939 Size: 5614 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2656 4728 4912 1305 Size: 1351 2072 184 2007 Fragments arranged by size: 2072 2007 1351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3792 Fragments arranged by size: 3792 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 3572 3931 4090 518 Size: 648 1002 1404 359 159 2042 Fragments arranged by size: 2042 1404 1002 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2037 3030 3707 4855 5100 1606 Size: 407 24 993 677 1148 245 2120 Fragments arranged by size: 2120 1148 993 677 407 245 24 NcoI C'CATG_G Cuts at: 513 1065 2004 2805 513 Size: 552 939 801 3322 Fragments arranged by size: 3322 939 801 552 NdeI CA'TA_TG Cuts at: 387 2305 387 Size: 1918 3696 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2972 4517 1132 Size: 97 1743 1545 2229 Fragments arranged by size: 2229 1743 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 4513 4513 Size: 5614 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5614 PfoI T'CCnGG_A Cuts at: 2328 2328 Size: 5614 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 2537 3184 2537 Size: 647 4967 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2840 2872 2840 Size: 32 5582 PstI C_TGCA'G Cuts at: 830 2453 2727 2757 2772 2966 830 Size: 1623 274 30 15 194 3478 Fragments arranged by size: 3478 1623 274 194 30 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 PvuII CAG'CTG Cuts at: 2037 2037 Size: 5614 SacII CC_GC'GG Cuts at: 2014 2014 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SfcI C'TryA_G Cuts at: 826 1045 2449 2723 2753 2768 2962 4097 4778 Size: 219 1404 274 30 15 194 1135 681 Cuts at: 4778 4969 826 Size: 191 1471 Fragments arranged by size: 1471 1404 1135 681 274 219 194 191 30 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3718 4619 4881 820 Size: 197 926 1775 901 262 1553 Fragments arranged by size: 1775 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2122 3405 493 Size: 1629 1283 2702 Fragments arranged by size: 2702 1629 1283 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 2127 3538 5 Size: 47 2075 1411 2081 Fragments arranged by size: 2081 2075 1411 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2004 2022 2589 2805 3053 513 Size: 552 939 18 567 216 248 3074 Fragments arranged by size: 3074 939 567 552 248 216 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2229 3641 3658 3811 3996 4441 325 Size: 1255 649 1412 17 153 185 445 1498 Fragments arranged by size: 1498 1412 1255 649 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2308 2337 3860 Size: 274 80 33 51 1443 331 29 1523 Cuts at: 3860 5572 96 Size: 1712 138 Fragments arranged by size: 1712 1523 1443 331 274 138 80 51 33 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5574 5606 5574 Size: 32 5582 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2803 2803 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 2045 3743 2045 Size: 1698 3916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI