(Circular) (Six-base) MAPSORT of: pFN21AB8179.seq Check: 4432 from: 1 to: 6007 pFN21AB8179 6007 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2898 2898 Size: 6007 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3164 3817 278 Size: 53 83 186 936 1628 653 2468 Fragments arranged by size: 2468 1628 936 653 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 3120 3328 3120 Size: 208 5799 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 3269 4070 5454 5722 1632 Size: 720 917 801 1384 268 1917 Fragments arranged by size: 1917 1384 917 801 720 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 4906 5883 1203 Size: 3703 977 1327 Fragments arranged by size: 3703 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 2256 1166 Size: 1090 4917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 2227 2392 2485 2680 2907 3750 5322 2227 Size: 165 93 195 227 843 1572 2912 Fragments arranged by size: 2912 1572 843 227 195 165 93 ApaLI G'TGCA_C Cuts at: 3055 4064 5220 3055 Size: 1009 1156 3842 Fragments arranged by size: 3842 1156 1009 ApoI r'AATT_y Cuts at: 1493 3316 3542 3694 5803 1493 Size: 1823 226 152 2109 1697 Fragments arranged by size: 2109 1823 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3234 4561 160 Size: 872 2202 1327 1606 Fragments arranged by size: 2202 1606 1327 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2265 2934 3210 3332 1870 Size: 73 322 669 276 122 4545 Fragments arranged by size: 4545 669 322 276 122 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2495 2528 2495 Size: 33 5974 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3120 3328 4783 618 Size: 325 217 627 1333 208 1455 1842 Fragments arranged by size: 1842 1455 1333 627 325 217 208 BanII G_rGCy'C Cuts at: 1391 3326 1391 Size: 1935 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5036 BbvCI CC'TCA_GC Cuts at: 2774 2774 Size: 6007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3286 3320 4198 4232 1903 Size: 34 1349 34 878 34 3678 Fragments arranged by size: 3678 1349 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 3899 5109 2272 Size: 1627 1210 3170 Fragments arranged by size: 3170 1627 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2427 2916 6002 2427 Size: 489 3086 2432 Fragments arranged by size: 3086 2432 489 BlpI GC'TnA_GC Cuts at: 2127 2528 3424 2127 Size: 401 896 4710 Fragments arranged by size: 4710 896 401 Bme1580I G_kGCm'C Cuts at: 1790 2378 3059 4068 5224 1790 Size: 588 681 1009 1156 2573 Fragments arranged by size: 2573 1156 1009 681 588 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2917 3069 3738 Size: 416 53 83 399 24 1500 152 669 Cuts at: 3738 4696 442 Size: 958 1753 Fragments arranged by size: 1753 1500 958 669 416 399 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3236 3236 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2132 2292 2723 2786 4667 1239 Size: 872 21 160 431 63 1881 2579 Fragments arranged by size: 2579 1881 872 431 160 63 21 Bpu10I CC'TnA_GC Cuts at: 2774 2978 2774 Size: 204 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4132 4997 5295 4132 Size: 865 298 4844 Fragments arranged by size: 4844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2918 4670 882 Size: 617 1419 1752 2219 Fragments arranged by size: 2219 1752 1419 617 BsaAI yAC'GTr Cuts at: 493 1204 2110 2298 3798 493 Size: 711 906 188 1500 2702 Fragments arranged by size: 2702 1500 906 711 188 BsaBI GATnn'nnATC Cuts at: 2495 3026 6001 2495 Size: 531 2975 2501 Fragments arranged by size: 2975 2501 531 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2940 3161 3814 4196 Size: 53 83 186 936 1407 221 653 382 Cuts at: 4196 4831 275 Size: 635 1451 Fragments arranged by size: 1451 1407 936 653 635 382 221 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5782 Fragments arranged by size: 5782 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2390 2555 1186 Size: 922 282 165 4638 Fragments arranged by size: 4638 922 282 165 BseYI C'CCAG_C Cuts at: 2336 2860 2953 5210 5706 2336 Size: 524 93 2257 496 2637 Fragments arranged by size: 2637 2257 524 496 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2240 2609 2699 2708 2795 1163 Size: 573 351 153 369 90 9 87 4375 Fragments arranged by size: 4375 573 369 351 153 90 87 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2297 2328 3059 4068 4153 5224 2297 Size: 31 731 1009 85 1071 3080 Fragments arranged by size: 3080 1071 1009 731 85 31 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 BsmBI CGTCTCn'nnnn_ Cuts at: 2668 2668 Size: 6007 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2297 2328 2378 3059 3326 4068 4153 Size: 399 507 31 50 681 267 742 85 Cuts at: 4153 5224 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 681 507 399 267 85 50 31 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2862 2898 3362 844 Size: 2018 36 464 3489 Fragments arranged by size: 3489 2018 464 36 BsrBI CCG'CTC Cuts at: 1394 1823 2083 3894 1394 Size: 429 260 1811 3507 Fragments arranged by size: 3507 1811 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2302 4651 4838 1700 Size: 110 492 2349 187 2869 Fragments arranged by size: 2869 2349 492 187 110 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 3760 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 2485 2524 2680 2722 3114 794 Size: 1691 39 156 42 392 3687 Fragments arranged by size: 3687 1691 392 156 42 39 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstYI r'GATC_y Cuts at: 1972 2427 2481 2823 2916 3337 3704 4089 4106 Size: 455 54 342 93 421 367 385 17 Cuts at: 4106 5558 5644 5656 6002 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 455 421 385 367 346 342 93 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3267 513 Size: 552 939 1263 3253 Fragments arranged by size: 3253 1263 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 2018 2263 3059 3523 4317 4337 2018 Size: 245 796 464 794 20 3688 Fragments arranged by size: 3688 796 794 464 245 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DraIII CAC_nnn'GTG Cuts at: 2110 3130 2110 Size: 1020 4987 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2300 2493 Size: 54 1425 50 275 18 303 167 193 Cuts at: 2493 2535 3196 3454 4343 5972 8 Size: 42 661 258 889 1629 43 Fragments arranged by size: 1629 1425 889 661 303 275 258 193 167 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3937 1353 Size: 2584 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2723 3140 4594 4968 5114 387 Size: 1064 138 1134 417 1454 374 146 1280 Fragments arranged by size: 1454 1280 1134 1064 417 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2132 2292 2352 2723 2786 3269 Size: 393 479 21 160 60 371 63 483 Cuts at: 3269 4070 4667 5454 5722 1239 Size: 801 597 787 268 1524 Fragments arranged by size: 1524 801 787 597 483 479 393 371 268 160 63 60 21 EcoO109I rG'GnC_Cy Cuts at: 2044 2154 2389 2642 2750 2770 2044 Size: 110 235 253 108 20 5281 Fragments arranged by size: 5281 253 235 110 108 20 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2680 3011 5154 1678 Size: 1002 331 2143 2531 Fragments arranged by size: 2531 2143 1002 331 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373 Size: 32 135 32 3076 32 42 32 2626 Fragments arranged by size: 3076 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3351 3597 669 Size: 988 281 1413 246 3079 Fragments arranged by size: 3079 1413 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 3124 3332 3124 Size: 208 5799 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2631 3195 5121 5305 1305 Size: 1326 564 1926 184 2007 Fragments arranged by size: 2007 1926 1326 564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2495 3198 10 Size: 54 1425 343 303 360 703 2819 Fragments arranged by size: 2819 1425 703 360 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2256 3965 4324 4483 518 Size: 648 1090 1709 359 159 2042 Fragments arranged by size: 2042 1709 1090 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 2764 2818 3049 3423 4100 5248 5493 Size: 513 645 54 231 374 677 1148 245 Cuts at: 5493 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 645 513 374 245 231 54 NaeI GCC'GGC Cuts at: 2304 2304 Size: 6007 NcoI C'CATG_G Cuts at: 513 1065 2004 3267 513 Size: 552 939 1263 3253 Fragments arranged by size: 3253 1263 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NgoMIV G'CCGG_C Cuts at: 2302 2302 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2527 3365 4910 1132 Size: 97 1298 838 1545 2229 Fragments arranged by size: 2229 1545 1298 838 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 2893 1108 Size: 1785 4222 PfoI T'CCnGG_A Cuts at: 2613 2613 Size: 6007 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PmlI CAC'GTG Cuts at: 2298 2298 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2405 2437 4076 4108 5589 5621 2405 Size: 32 1639 32 1481 32 2791 Fragments arranged by size: 2791 1639 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2044 2154 2642 2044 Size: 110 488 5409 Fragments arranged by size: 5409 488 110 PshAI GACnn'nnGTC Cuts at: 1613 2640 1613 Size: 1027 4980 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PstI C_TGCA'G Cuts at: 830 2052 2490 3359 830 Size: 1222 438 869 3478 Fragments arranged by size: 3478 1222 869 438 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2119 2764 2119 Size: 645 5362 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SanDI GG'GwC_CC Cuts at: 2154 2154 Size: 6007 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2048 2486 3355 4490 5171 5362 826 Size: 219 1003 438 869 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 869 681 438 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 2936 3334 1872 Size: 1064 398 4545 Fragments arranged by size: 4545 1064 398 SmlI C'TyrA_G Cuts at: 820 1017 1943 4111 5012 5274 820 Size: 197 926 2168 901 262 1553 Fragments arranged by size: 2168 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 2527 3365 1132 Size: 1395 838 3774 Fragments arranged by size: 3774 1395 838 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6007 StyI C'CwwG_G Cuts at: 513 1065 2004 3267 3446 513 Size: 552 939 1263 179 3074 Fragments arranged by size: 3074 1263 939 552 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4034 4051 4204 4389 4834 325 Size: 1255 2454 17 153 185 445 1498 Fragments arranged by size: 2454 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 4253 5965 Size: 274 80 33 51 1443 366 1910 1712 Cuts at: 5965 96 Size: 138 Fragments arranged by size: 1910 1712 1443 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5967 5999 5967 Size: 32 5975 Tth111I GACn'n_nGTC Cuts at: 1316 2042 1316 Size: 726 5281 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2316 2176 Size: 140 5867 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 2934 3332 1870 Size: 1064 398 4545 Fragments arranged by size: 4545 1064 398 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3162 3815 276 Size: 53 83 186 936 1628 653 2468 Fragments arranged by size: 2468 1628 936 653 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI