(Circular) (Six-base) MAPSORT of: pFN21AB8176.seq Check: 6766 from: 1 to: 5440 pFN21AB8176 5440 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2084 2084 Size: 5440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2385 2761 2385 Size: 376 5064 AclI AA'CG_TT Cuts at: 1563 2739 3567 3940 1563 Size: 1176 828 373 3063 Fragments arranged by size: 3063 1176 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2415 3503 4887 5155 1632 Size: 783 1088 1384 268 1917 Fragments arranged by size: 1917 1384 1088 783 268 AfeI AGC'GCT Cuts at: 2057 2057 Size: 5440 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 2291 1166 Size: 1125 4315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2309 1184 Size: 1125 4315 AlwNI CAG_nnn'CTG Cuts at: 3183 4755 3183 Size: 1572 3868 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2765 1870 Size: 73 822 4545 Fragments arranged by size: 4545 822 73 BamHI G'GATC_C Cuts at: 2770 3137 2770 Size: 367 5073 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2385 2761 4216 618 Size: 325 217 627 315 283 376 1455 1842 Fragments arranged by size: 1842 1455 627 376 325 315 283 217 BanII G_rGCy'C Cuts at: 1391 2759 1391 Size: 1368 4072 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 5440 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2018 2213 2344 2634 928 Size: 971 119 195 131 290 3734 Fragments arranged by size: 3734 971 290 195 131 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 2048 4051 Size: 107 122 71 631 605 367 9 2003 Cuts at: 4051 136 Size: 1525 Fragments arranged by size: 2003 1525 631 605 367 122 107 71 9 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 3501 4657 1790 Size: 1711 1156 2573 Fragments arranged by size: 2573 1711 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 4100 1239 Size: 961 1900 2579 Fragments arranged by size: 2579 1900 961 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3565 4430 4728 3565 Size: 865 298 4277 Fragments arranged by size: 4277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 3247 3629 4264 Size: 53 83 186 936 570 1144 382 635 Cuts at: 4264 275 Size: 1451 Fragments arranged by size: 1451 1144 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 3873 4271 4545 4692 5103 2382 Size: 1491 398 274 147 411 2719 Fragments arranged by size: 2719 1491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 1178 Size: 30 165 30 717 30 4468 Fragments arranged by size: 4468 717 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 1186 Size: 949 4491 BseYI C'CCAG_C Cuts at: 4643 5139 4643 Size: 496 4944 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2269 2554 2721 1163 Size: 573 533 285 167 3882 Fragments arranged by size: 3882 573 533 285 167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2890 3651 4679 1540 Size: 275 186 889 761 1028 2301 Fragments arranged by size: 2301 1028 889 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3501 3586 4657 3501 Size: 85 1071 4284 Fragments arranged by size: 4284 1071 85 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2759 3501 3586 4657 1391 Size: 399 969 742 85 1071 2174 Fragments arranged by size: 2174 1071 969 742 399 85 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2084 2139 2160 2795 844 Size: 1240 55 21 635 3489 Fragments arranged by size: 3489 1240 635 55 21 BsrBI CCG'CTC Cuts at: 1394 1823 3327 1394 Size: 429 1504 3507 Fragments arranged by size: 3507 1504 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2601 3935 4109 65 Size: 1183 1353 1334 174 1396 Fragments arranged by size: 1396 1353 1334 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 3500 4512 3500 Size: 1012 4428 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5440 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2276 2770 3137 3522 3539 4991 5077 5089 Size: 304 494 367 385 17 1452 86 12 Cuts at: 5089 5435 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 494 385 367 346 304 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 2289 513 Size: 552 939 135 150 3664 Fragments arranged by size: 3664 939 552 150 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2094 2956 3750 3770 2094 Size: 862 794 20 3764 Fragments arranged by size: 3764 862 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2070 2887 Size: 54 1425 50 275 18 210 30 817 Cuts at: 2887 3776 5405 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 817 275 210 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2887 1537 Size: 275 1075 4090 Fragments arranged by size: 4090 1075 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3370 1353 Size: 2017 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2164 2492 4027 4401 4547 387 Size: 1064 138 575 328 1535 374 146 1280 Fragments arranged by size: 1535 1280 1064 575 374 328 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2415 3503 4100 4887 5155 1239 Size: 393 568 215 1088 597 787 268 1524 Fragments arranged by size: 1524 1088 787 597 568 393 268 215 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 2059 2106 4587 1678 Size: 381 47 2481 2531 Fragments arranged by size: 2531 2481 381 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 4081 4113 4155 Size: 32 135 32 689 32 1788 32 42 Cuts at: 4155 4187 1373 Size: 32 2626 Fragments arranged by size: 2626 1788 689 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KasI G'GCGC_C Cuts at: 2102 2102 Size: 5440 KpnI G_GTAC'C Cuts at: 2389 2765 2389 Size: 376 5064 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 2244 2286 4554 4738 1305 Size: 834 105 42 2268 184 2007 Fragments arranged by size: 2268 2007 834 184 105 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 10 Size: 54 1425 343 240 3378 Fragments arranged by size: 3378 1425 343 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2291 3398 3757 3916 518 Size: 648 912 213 1107 359 159 2042 Fragments arranged by size: 2042 1107 912 648 359 213 159 MspA1I CmG'CkG Cuts at: 1606 2236 2856 3533 4681 4926 1606 Size: 630 620 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 620 245 NarI GG'CG_CC Cuts at: 2103 2103 Size: 5440 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3949 Fragments arranged by size: 3949 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2798 4343 1132 Size: 97 1569 1545 2229 Fragments arranged by size: 2229 1569 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5440 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PstI C_TGCA'G Cuts at: 830 2792 830 Size: 1962 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 PvuII CAG'CTG Cuts at: 2236 2236 Size: 5440 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2788 3923 4604 4795 826 Size: 219 1743 1135 681 191 1471 Fragments arranged by size: 1743 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2039 2048 2039 Size: 9 5431 SfoI GGC'GCC Cuts at: 2104 2104 Size: 5440 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3544 4445 4707 820 Size: 197 926 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 2315 3364 5 Size: 47 2263 1049 2081 Fragments arranged by size: 2263 2081 1049 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5440 StyI C'CwwG_G Cuts at: 513 1065 2004 2440 2879 513 Size: 552 939 436 439 3074 Fragments arranged by size: 3074 939 552 439 436 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 2225 2511 3467 3484 3637 3822 Size: 1255 528 117 286 956 17 153 185 Cuts at: 3822 4267 325 Size: 445 1498 Fragments arranged by size: 1498 1255 956 528 445 286 185 153 117 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2484 2516 5400 5432 2484 Size: 32 2884 32 2492 Fragments arranged by size: 2884 2492 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI XcmI