Restriction map for
pFN21AB8173
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AvaI
AviII AvrII BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI BspEI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp509I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 292 GACGTC GACGT^C
AcyI 292 GACGTC G[GA]^CG[CT]C
AflIII 1005 ACACGT A^C[GA][CT]GT
AhdI 597 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 97 GGGCCC GGGCC^C
AspEI 597 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 764 TGGCCA TGG^CCA
BbrPI 1006 CACGTG CAC^GTG
BfaI 65 CTAG C^TAG
BglI 962 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 176 AGATCT A^GATCT
BsaAI 1006 CACGTG [CT]AC^GT[GA]
BsaBI 453 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 292 GACGTC G[GA]^CG[CT]C
BsaI 563 GAGACC GGTCTC(1/5)
BsgI 950 GTGCAG GTGCAG(16/14)
Bsp1407I 585 TGTACA T^GTACA
BspDI 922 ATCGAT AT^CGAT
BsrBI 190 CCGCTC CCGCTC(-3/-3)
BsrFI 113 ACCGGC [GA]^CCGG[CT]
BsrGI 585 TGTACA T^GTACA
BstXI 458 CCATCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 844 GCGC GCG^C
Cfr10I 113 ACCGGC [GA]^CCGG[CT]
ClaI 922 ATCGAT AT^CGAT
DdeI 353 CTGAG C^T[ACGT]AG
Eam1105I 597 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI 844 GCGC GCG^C
Hin1I 292 GACGTC G[GA]^CG[CT]C
HinP1I 844 GCGC G^CGC
HincII 874 GTCAAC GT[CT]^[GA]AC
HindII 874 GTCAAC GT[CT]^[GA]AC
MaeI 65 CTAG C^TAG
MamI 453 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 764 TGGCCA TGG^CCA
MscI 764 TGGCCA TGG^CCA
MseI 890 TTAA T^TAA
MspA1I 838 CAGCTG C[AC]G^C[GT]G
NheI 64 GCTAGC G^CTAGC
NspI 238 ACATGC [GA]CATG^[CT]
PflFI 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1006 CACGTG CAC^GTG
PmlI 1006 CACGTG CAC^GTG
PpuMI 595 GGGACCC [GA]G^G[AT]CC[CT]
PstI 492 CTGCAG CTGCA^G
PvuII 838 CAGCTG CAG^CTG
SapI 192 GCTCTTC GCTCTTC(1/4)
SexAI 464 ACCTGGT A^CC[AT]GGT
SfcI 492 CTGCAG C^T[GA][CT]AG
SspBI 585 TGTACA T^GTACA
Tru9I 890 TTAA T^TAA
Tth111I 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 124 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
412 CAGGTGCTG
BanII 97 GGGCCC G[GA]GC[CT]^C
856 GGGCTC
BspMI 428 ACCTGC ACCTGC(4/8)
342 GCAGGT
BstYI 176 AGATCT [GA]^GATC[CT]
749 GGATCT
EaeI 764 TGGCCA [CT]^GGCC[GA]
1010 TGGCCG
EcoT14I 654 CCAAGG C^C[AT][AT]GG
758 CCAAGG
HgaI 902 GACGC GACGC(5/10)
996 GACGC
HinfI 307 GATTC G^A[ACGT]TC
558 GATTC
HphI 289 GGTGA GGTGA(8/7)
860 TCACC
MaeII 293 ACGT A^CGT
1007 ACGT
MflI 176 AGATCT [GA]^GATC[CT]
749 GGATCT
MslI 408 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
672 CACCAGCGTG
PflMI 426 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
607 CCATCCAGTGG
StyI 654 CCAAGG C^C[AT][AT]GG
758 CCAAGG
TfiI 307 GATTC G^A[AT]TC
558 GATTC
Van91I 426 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
607 CCATCCAGTGG
XhoII 176 AGATCT [GA]^GATC[CT]
749 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
Ksp632I MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
TspRI TthHB8I XcmI