Restriction map for
pFN21AB8173
(Circular) (Six-base) MAPSORT of: pFN21AB8173.seq Check: 9522 from: 1 to: 5779
pFN21AB8173 5779 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2442 2442
Size: 5779
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2301 3589 278
Size: 53 83 186 936 765 1288 2468
Fragments arranged by size:
2468 1288 936 765 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2947 3842 5226 5494 1632
Size: 551 764 895 1384 268 1917
Fragments arranged by size:
1917 1384 895 764 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 3010 4678 5655 1203
Size: 1807 1668 977 1327
Fragments arranged by size:
1807 1668 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 2607 4508 2607
Size: 1901 3878
AleI CACnn'nnGTG
Cuts at: 1166 2681 1166
Size: 1515 4264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 2134 2422 3522 5094 2134
Size: 288 1100 1572 2819
Fragments arranged by size:
2819 1572 1100 288
ApaI G_GGCC'C
Cuts at: 2106 2106
Size: 5779
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 3104 1870
Size: 73 1161 4545
Fragments arranged by size:
4545 1161 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3100 4555 618
Size: 325 217 627 1313 1455 1842
Fragments arranged by size:
1842 1455 1313 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2106 2865 3098 1391
Size: 715 759 233 4072
Fragments arranged by size:
4072 759 715 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2973 4390 136
Size: 107 122 71 631 605 1301 1417 1525
Fragments arranged by size:
1525 1417 1301 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2181 5774 2181
Size: 3593 2186
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2106 3840 4996 1790
Size: 301 15 1734 1156 2573
Fragments arranged by size:
2573 1734 1156 301 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2688 3510 4468
Size: 416 53 83 399 24 1271 822 958
Cuts at: 4468 442
Size: 1753
Fragments arranged by size:
1753 1271 958 822 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2073 1056
Size: 1017 4762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 2459 2465 2635 4439 1239
Size: 1071 149 6 170 1804 2579
Fragments arranged by size:
2579 1804 1071 170 149 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2562 4442 882
Size: 617 1063 1880 2219
Fragments arranged by size:
2219 1880 1063 617
BsaAI yAC'GTr
Cuts at: 493 1204 3013 3570 493
Size: 711 1809 557 2702
Fragments arranged by size:
2702 1809 711 557
BsaBI GATnn'nnATC
Cuts at: 2462 5773 2462
Size: 3311 2468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 3586 3968 4603
Size: 53 83 186 936 765 1288 382 635
Cuts at: 4603 275
Size: 1451
Fragments arranged by size:
1451 1288 936 765 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2233 2263 2283 2288 2313
Size: 30 165 30 830 30 20 5 25
Cuts at: 2313 2318 2510 2540 1178
Size: 5 192 30 4417
Fragments arranged by size:
4417 830 192 165 30 30 30 30
25 20 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 2365 2843 1186
Size: 1110 69 478 4122
Fragments arranged by size:
4122 1110 478 69
BseYI C'CCAG_C
Cuts at: 2127 2270 2275 2521 2534 4982 5478 2127
Size: 143 5 246 13 2448 496 2428
Fragments arranged by size:
2448 2428 496 246 143 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2976 1163
Size: 573 1240 3966
Fragments arranged by size:
3966 1240 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3229 3990 5018 1540
Size: 275 186 1228 761 1028 2301
Fragments arranged by size:
2301 1228 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3840 3925 4996 3840
Size: 85 1071 4623
Fragments arranged by size:
4623 1071 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
BsmBI CGTCTCn'nnnn_
Cuts at: 2658 2899 2993 2658
Size: 241 94 5444
Fragments arranged by size:
5444 241 94
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2106 2865 3098 3840 3925 4996
Size: 399 301 15 759 233 742 85 1071
Cuts at: 4996 1391
Size: 2174
Fragments arranged by size:
2174 1071 759 742 399 301 233 85
15
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2338 2442 3134 844
Size: 1494 104 692 3489
Fragments arranged by size:
3489 1494 692 104
BsrBI CCG'CTC
Cuts at: 1394 1823 2197 3666 1394
Size: 429 374 1469 3507
Fragments arranged by size:
3507 1469 429 374
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2118 4423 4610 1700
Size: 110 308 2305 187 2869
Fragments arranged by size:
2869 2305 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 2590 96
Size: 2494 3285
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 2470
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2181 2754 3109 3476 3861 3878 5330 5416
Size: 209 573 355 367 385 17 1452 86
Cuts at: 5416 5428 5774 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 573 385 367 355 346 209
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2314 487
Size: 683 56 1088 3952
Fragments arranged by size:
3952 1088 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 2928 1554
Size: 1374 4405
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2769 3015 3226
Size: 54 1425 50 275 18 939 246 211
Cuts at: 3226 4115 5744 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 939 889 275 246 211 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2204 2375 2741 3709 1353
Size: 806 45 171 366 968 3423
Fragments arranged by size:
3423 968 806 366 171 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2310 2459 2465 2635 2947 3842
Size: 393 551 127 149 6 170 312 895
Cuts at: 3842 4439 5226 5494 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 895 787 597 551 393 312 268
170 149 127 6
EcoO109I rG'GnC_Cy
Cuts at: 2102 2103 2364 2601 2623 2102
Size: 1 261 237 22 5258
Fragments arranged by size:
5258 261 237 22 1
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 4926 1678
Size: 3248 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2283 2288 2315
Size: 32 135 32 609 32 70 5 27
Cuts at: 2315 2320 2570 2593 2602 2625 4420 4452 4494
Size: 5 250 23 9 23 1795 32 42
Cuts at: 4494 4526 1373
Size: 32 2626
Fragments arranged by size:
2626 1795 609 250 135 70 42 32
32 32 32 32 27 23 23 9
5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2881 3123 3369 669
Size: 988 281 943 242 246 3079
Fragments arranged by size:
3079 988 943 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 2961 4893 5077 1305
Size: 1098 558 1932 184 2007
Fragments arranged by size:
2007 1932 1098 558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2771 10
Size: 54 1425 343 939 3018
Fragments arranged by size:
3018 1425 939 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2681 3737 4096 4255 518
Size: 648 1251 264 1056 359 159 2042
Fragments arranged by size:
2042 1251 1056 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2845 3195 3872 5020 5265 1606
Size: 1239 350 677 1148 245 2120
Fragments arranged by size:
2120 1239 1148 677 350 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 2069 1052
Size: 1017 4762
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2247 3137 4682 1132
Size: 97 1018 890 1545 2229
Fragments arranged by size:
2229 1545 1018 890 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 2618 1108
Size: 1329 181 4269
Fragments arranged by size:
4269 1329 181
PfoI T'CCnGG_A
Cuts at: 3028 3028
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PmlI CAC'GTG
Cuts at: 3013 3013
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2601 2601
Size: 5779
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2102 2102
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2501 3131 830
Size: 1671 630 3478
Fragments arranged by size:
3478 1671 630
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2845 2845
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SanDI GG'GwC_CC
Cuts at: 2601 2601
Size: 5779
SapI GCTCTTCn'nnn_
Cuts at: 2204 2204
Size: 5779
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SexAI A'CCwGG_T
Cuts at: 2469 2469
Size: 5779
SfcI C'TryA_G
Cuts at: 826 1045 2497 3127 4262 4943 5134 826
Size: 219 1452 630 1135 681 191 1471
Fragments arranged by size:
1471 1452 1135 681 630 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2004 2659 2763 3218 513
Size: 552 939 655 104 455 3074
Fragments arranged by size:
3074 939 655 552 455 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2590 4025 5737
Size: 274 80 33 51 1443 613 1435 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1712 1443 1435 613 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 2471 1316
Size: 1155 4624
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2579 2623 2386
Size: 193 44 5542
Fragments arranged by size:
5542 193 44
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2299 3587 276
Size: 53 83 186 936 765 1288 2468
Fragments arranged by size:
2468 1288 936 765 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacI SacII
SfiI SfoI SrfI SwaI