Restriction map for
pFN21AB8173
(Linear) (Six-base) MAPSORT of: KIBB8173.seq Check: 3533 from: 1 to: 1074
KIBB8173.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 437 1074
Size: 437 637
AatII G_ACGT'C
Cuts at: 0 296 1074
Size: 296 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 942 1074
Size: 178 764 132
Fragments arranged by size:
764 178 132
AflIII A'CryG_T
Cuts at: 0 1005 1074
Size: 1005 69
AhdI GACnn_n'nnGTC
Cuts at: 0 602 1074
Size: 602 472
AleI CACnn'nnGTG
Cuts at: 0 676 1074
Size: 676 398
AlwNI CAG_nnn'CTG
Cuts at: 0 129 417 1074
Size: 129 288 657
Fragments arranged by size:
657 288 129
ApaI G_GGCC'C
Cuts at: 0 101 1074
Size: 101 973
BanII G_rGCy'C
Cuts at: 0 101 860 1074
Size: 101 759 214
Fragments arranged by size:
759 214 101
BglI GCCn_nnn'nGGC
Cuts at: 0 968 1074
Size: 968 106
BglII A'GATC_T
Cuts at: 0 176 1074
Size: 176 898
Bme1580I G_kGCm'C
Cuts at: 0 86 101 1074
Size: 86 15 973
Fragments arranged by size:
973 86 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 683 1074
Size: 683 391
BmtI G_CTAG'C
Cuts at: 0 68 1074
Size: 68 1006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 454 460 630 1074
Size: 305 149 6 170 444
Fragments arranged by size:
444 305 170 149 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 557 1074
Size: 557 517
BsaAI yAC'GTr
Cuts at: 0 1008 1074
Size: 1008 66
BsaBI GATnn'nnATC
Cuts at: 0 457 1074
Size: 457 617
BsaHI Gr'CG_yC
Cuts at: 0 293 1074
Size: 293 781
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 228 258 278 283 308 313 505 535
Size: 228 30 20 5 25 5 192 30
Cuts at: 535 1074
Size: 539
Fragments arranged by size:
539 228 192 30 30 25 20 5
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 360 838 1074
Size: 291 69 478 236
Fragments arranged by size:
478 291 236 69
BseYI C'CCAG_C
Cuts at: 0 122 265 270 516 529 1074
Size: 122 143 5 246 13 545
Fragments arranged by size:
545 246 143 122 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1074
Size: 971 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 653 894 988 1074
Size: 653 241 94 86
Fragments arranged by size:
653 241 94 86
Bsp1286I G_dGCh'C
Cuts at: 0 86 101 860 1074
Size: 86 15 759 214
Fragments arranged by size:
759 214 86 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 437 1074
Size: 333 104 637
Fragments arranged by size:
637 333 104
BsrBI CCG'CTC
Cuts at: 0 192 1074
Size: 192 882
BsrFI r'CCGG_y
Cuts at: 0 113 1074
Size: 113 961
BsrGI T'GTAC_A
Cuts at: 0 585 1074
Size: 585 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 1074
Size: 465 609
BstYI r'GATC_y
Cuts at: 0 176 749 1074
Size: 176 573 325
Fragments arranged by size:
573 325 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 309 1074
Size: 309 765
ClaI AT'CG_AT
Cuts at: 0 923 1074
Size: 923 151
EaeI y'GGCC_r
Cuts at: 0 764 1010 1074
Size: 764 246 64
Fragments arranged by size:
764 246 64
EarI CTCTTCn'nnn_
Cuts at: 0 154 199 370 736 1074
Size: 154 45 171 366 338
Fragments arranged by size:
366 338 171 154 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 305 454 460 630 942 1074
Size: 178 127 149 6 170 312 132
Fragments arranged by size:
312 178 170 149 132 127 6
EcoO109I rG'GnC_Cy
Cuts at: 0 97 98 359 596 618 1074
Size: 97 1 261 237 22 456
Fragments arranged by size:
456 261 237 97 22 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 278 283 310 315 565 588
Size: 176 32 70 5 27 5 250 23
Cuts at: 588 597 620 1074
Size: 9 23 454
Fragments arranged by size:
454 250 176 70 32 27 23 23
9 5 5
HincII GTy'rAC
Cuts at: 0 876 1074
Size: 876 198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 956 1074
Size: 398 558 118
Fragments arranged by size:
558 398 118
MscI TGG'CCA
Cuts at: 0 766 1074
Size: 766 308
MslI CAynn'nnrTG
Cuts at: 0 412 676 1074
Size: 412 264 398
Fragments arranged by size:
412 398 264
MspA1I CmG'CkG
Cuts at: 0 840 1074
Size: 840 234
NheI G'CTAG_C
Cuts at: 0 64 1074
Size: 64 1010
NspI r_CATG'y
Cuts at: 0 242 1074
Size: 242 832
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 613 1074
Size: 432 181 461
Fragments arranged by size:
461 432 181
PfoI T'CCnGG_A
Cuts at: 0 1023 1074
Size: 1023 51
PmlI CAC'GTG
Cuts at: 0 1008 1074
Size: 1008 66
PpuMI rG'GwC_Cy
Cuts at: 0 596 1074
Size: 596 478
PspOMI G'GGCC_C
Cuts at: 0 97 1074
Size: 97 977
PstI C_TGCA'G
Cuts at: 0 496 1074
Size: 496 578
PvuII CAG'CTG
Cuts at: 0 840 1074
Size: 840 234
SanDI GG'GwC_CC
Cuts at: 0 596 1074
Size: 596 478
SapI GCTCTTCn'nnn_
Cuts at: 0 199 1074
Size: 199 875
SexAI A'CCwGG_T
Cuts at: 0 464 1074
Size: 464 610
SfcI C'TryA_G
Cuts at: 0 492 1074
Size: 492 582
StyI C'CwwG_G
Cuts at: 0 654 758 1074
Size: 654 104 316
Fragments arranged by size:
654 316 104
TatI w'GTAC_w
Cuts at: 0 585 1074
Size: 585 489
Tth111I GACn'n_nGTC
Cuts at: 0 466 1074
Size: 466 608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 574 618 1074
Size: 381 193 44 456
Fragments arranged by size:
456 381 193 44
ZraI GAC'GTC
Cuts at: 0 294 1074
Size: 294 780
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlwNI ApaI
BanII BglI BglII Bme1580I BmrI BmtI BpmI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BstXI BstYI BtgZI
ClaI EaeI EarI Eco57MI EcoO109I Hin4I HincII MmeI
MscI MslI MspA1I NheI NspI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SanDI SapI SexAI SfcI
StyI TatI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI BmgBI BplI Bpu10I BpuEI BsaWI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtsI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PasI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XbaI XhoI XmaI XmnI