(Circular) (Six-base) MAPSORT of: pFN21AB8171.seq Check: 4899 from: 1 to: 6376 pFN21AB8171 6376 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2219 2219 Size: 6376 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3719 1937 Size: 1782 4594 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 2420 2466 2503 3418 3511 3600 4439 Size: 707 81 46 37 915 93 89 839 Cuts at: 4439 5823 6091 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 915 839 707 268 93 89 81 46 37 AflII C'TTAA_G Cuts at: 820 1017 2611 820 Size: 197 1594 4585 Fragments arranged by size: 4585 1594 197 AflIII A'CryG_T Cuts at: 1203 5275 6252 1203 Size: 4072 977 1327 Fragments arranged by size: 4072 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2962 2963 2994 2995 3480 3512 2962 Size: 1 31 1 485 32 5826 Fragments arranged by size: 5826 485 32 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2067 2275 2578 2849 3451 4119 5691 2067 Size: 208 303 271 602 668 1572 2752 Fragments arranged by size: 2752 1572 668 602 303 271 208 ApaLI G'TGCA_C Cuts at: 2350 2582 4433 5589 2350 Size: 232 1851 1156 3137 Fragments arranged by size: 3137 1851 1156 232 ApoI r'AATT_y Cuts at: 1493 2544 2647 2735 3174 3685 3911 4063 6172 Size: 1051 103 88 439 511 226 152 2109 Cuts at: 6172 1493 Size: 1697 Fragments arranged by size: 2109 1697 1051 511 439 226 152 103 88 AseI AT'TA_AT Cuts at: 160 1032 4930 160 Size: 872 3898 1606 Fragments arranged by size: 3898 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 3363 3701 1870 Size: 73 1420 338 4545 Fragments arranged by size: 4545 1420 338 73 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3095 3697 5152 618 Size: 325 217 627 1308 602 1455 1842 Fragments arranged by size: 1842 1455 1308 627 602 325 217 BanII G_rGCy'C Cuts at: 1391 3695 1391 Size: 2304 4072 BbeI G_GCGC'C Cuts at: 3099 3099 Size: 6376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3159 3200 928 Size: 971 1260 41 4104 Fragments arranged by size: 4104 1260 971 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4567 4601 1903 Size: 34 2630 34 3678 Fragments arranged by size: 3678 2630 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3096 4268 5478 3096 Size: 1172 1210 3994 Fragments arranged by size: 3994 1210 1172 BclI T'GATC_A Cuts at: 1548 1548 Size: 6376 BfrBI ATG'CAT Cuts at: 2884 2884 Size: 6376 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6371 6371 Size: 6376 BlpI GC'TnA_GC Cuts at: 3793 3793 Size: 6376 Bme1580I G_kGCm'C Cuts at: 1790 2354 2586 3525 4437 5593 1790 Size: 564 232 939 912 1156 2573 Fragments arranged by size: 2573 1156 939 912 564 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2057 2216 2710 Size: 416 53 83 399 24 640 159 494 Cuts at: 2710 4107 5065 442 Size: 1397 958 1753 Fragments arranged by size: 1753 1397 958 640 494 416 399 159 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3093 3534 5036 1239 Size: 1854 441 1502 2579 Fragments arranged by size: 2579 1854 1502 441 Bpu10I CC'TnA_GC Cuts at: 3213 3213 Size: 6376 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2138 4501 5366 5664 2138 Size: 2363 865 298 2850 Fragments arranged by size: 2850 2363 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2658 3127 5039 882 Size: 617 1159 469 1912 2219 Fragments arranged by size: 2219 1912 1159 617 469 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3096 4183 4565 5200 Size: 53 83 186 936 1563 1087 382 635 Cuts at: 5200 275 Size: 1451 Fragments arranged by size: 1563 1451 1087 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4809 5207 5481 5628 6039 4809 Size: 398 274 147 411 5146 Fragments arranged by size: 5146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2690 2720 3480 3510 1178 Size: 30 165 30 1287 30 760 30 4044 Fragments arranged by size: 4044 1287 760 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2778 1186 Size: 1090 502 4784 Fragments arranged by size: 4784 1090 502 BseYI C'CCAG_C Cuts at: 2617 5579 6075 2617 Size: 2962 496 2918 Fragments arranged by size: 2962 2918 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2288 2807 3189 1163 Size: 573 552 519 382 4350 Fragments arranged by size: 4350 573 552 519 382 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3826 4587 5615 1540 Size: 275 186 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2354 2586 4437 4522 5593 2354 Size: 232 1851 85 1071 3137 Fragments arranged by size: 3137 1851 1071 232 85 BsmI GAATG_Cn' Cuts at: 2483 2884 3887 3980 2483 Size: 401 1003 93 4879 Fragments arranged by size: 4879 1003 401 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2354 2586 3525 3695 4437 4522 5593 Size: 399 564 232 939 170 742 85 1071 Cuts at: 5593 1391 Size: 2174 Fragments arranged by size: 2174 1071 939 742 564 399 232 170 85 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 3127 4265 5995 3127 Size: 1138 1730 3508 Fragments arranged by size: 3508 1730 1138 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2219 3731 844 Size: 1375 1512 3489 Fragments arranged by size: 3489 1512 1375 BsrBI CCG'CTC Cuts at: 1394 1823 4263 1394 Size: 429 2440 3507 Fragments arranged by size: 3507 2440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 2775 3240 4871 5045 65 Size: 1183 1219 308 465 1631 174 1396 Fragments arranged by size: 1631 1396 1219 1183 465 308 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2676 5020 5207 1700 Size: 110 866 2344 187 2869 Fragments arranged by size: 2869 2344 866 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6376 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 2275 2349 2489 2581 794 Size: 1481 74 140 92 4589 Fragments arranged by size: 4589 1481 140 92 74 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 3477 1184 Size: 2293 4083 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2047 Size: 6376 BstYI r'GATC_y Cuts at: 1972 3706 4073 4458 4475 5927 6013 6025 6371 Size: 1734 367 385 17 1452 86 12 346 Cuts at: 6371 1972 Size: 1977 Fragments arranged by size: 1977 1734 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5824 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3288 487 Size: 683 56 2062 3575 Fragments arranged by size: 3575 2062 683 56 BtsI GCAGTG_nn' Cuts at: 2180 2795 2946 3892 4686 4706 2180 Size: 615 151 946 794 20 3850 Fragments arranged by size: 3850 946 794 615 151 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3223 3258 529 Size: 35 2659 35 3647 Fragments arranged by size: 3647 2659 35 35 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2144 5383 809 Size: 507 828 3239 1802 Fragments arranged by size: 3239 1802 828 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2359 3354 3823 Size: 54 1425 50 275 18 529 995 469 Cuts at: 3823 4712 6341 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 995 889 529 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4306 1353 Size: 2953 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4963 5337 5483 387 Size: 1064 138 3374 374 146 1280 Fragments arranged by size: 3374 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2420 2466 2503 3093 3418 3511 Size: 393 707 81 46 37 590 325 93 Cuts at: 3511 3534 3600 4439 5036 5823 6091 1239 Size: 23 66 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 707 597 590 393 325 268 93 81 66 46 37 23 EcoO109I rG'GnC_Cy Cuts at: 2962 2962 Size: 6376 EcoRI G'AATT_C Cuts at: 2735 3174 3685 4063 2735 Size: 439 511 378 5048 Fragments arranged by size: 5048 511 439 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2533 2740 2533 Size: 207 6169 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 2275 3099 5523 1678 Size: 597 824 2424 2531 Fragments arranged by size: 2531 2424 824 597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 2690 2722 2845 Size: 32 135 32 577 32 509 32 123 Cuts at: 2845 2877 3495 3527 5017 5049 5091 5123 1373 Size: 32 618 32 1490 32 42 32 2626 Fragments arranged by size: 2626 1490 618 577 509 135 123 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 3720 3966 669 Size: 988 281 250 1532 246 3079 Fragments arranged by size: 3079 1532 988 281 250 246 HindIII A'AGCT_T Cuts at: 748 3024 748 Size: 2276 4100 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KasI G'GCGC_C Cuts at: 3095 3095 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3244 5490 5674 1305 Size: 1939 2246 184 2007 Fragments arranged by size: 2246 2007 1939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2361 10 Size: 54 1425 343 529 4025 Fragments arranged by size: 4025 1425 529 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 3350 3410 3446 4334 4693 4852 Size: 648 1269 915 60 36 888 359 159 Cuts at: 4852 518 Size: 2042 Fragments arranged by size: 2042 1269 915 888 648 359 159 60 36 MspA1I CmG'CkG Cuts at: 1606 2036 2578 2661 2976 3554 3792 4469 5617 Size: 430 542 83 315 578 238 677 1148 Cuts at: 5617 5862 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 578 542 430 315 245 238 83 NaeI GCC'GGC Cuts at: 2678 2678 Size: 6376 NarI GG'CG_CC Cuts at: 3096 3096 Size: 6376 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5824 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NgoMIV G'CCGG_C Cuts at: 2676 2676 Size: 6376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NsiI A_TGCA'T Cuts at: 2886 2886 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3445 3734 5279 1132 Size: 97 2216 289 1545 2229 Fragments arranged by size: 2229 2216 1545 289 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 3598 1108 Size: 2490 3886 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3480 3512 4445 4477 5958 5990 3480 Size: 32 933 32 1481 32 3866 Fragments arranged by size: 3866 1481 933 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2962 2962 Size: 6376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PstI C_TGCA'G Cuts at: 830 3728 830 Size: 2898 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 2578 2976 3554 2578 Size: 398 578 5400 Fragments arranged by size: 5400 578 398 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SanDI GG'GwC_CC Cuts at: 2962 2962 Size: 6376 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 2937 3724 4859 5540 5731 826 Size: 219 1892 787 1135 681 191 1471 Fragments arranged by size: 1892 1471 1135 787 681 219 191 SfoI GGC'GCC Cuts at: 3097 3097 Size: 6376 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3365 3703 1872 Size: 1493 338 4545 Fragments arranged by size: 4545 1493 338 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 2611 4480 5381 5643 820 Size: 197 926 210 458 1869 901 262 1553 Fragments arranged by size: 1869 1553 926 901 458 262 210 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 4300 5 Size: 47 4248 2081 Fragments arranged by size: 4248 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2335 3294 3483 3815 513 Size: 552 1270 959 189 332 3074 Fragments arranged by size: 3074 1270 959 552 332 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2897 3496 4403 4420 4573 4758 5203 Size: 1255 1317 599 907 17 153 185 445 Cuts at: 5203 325 Size: 1498 Fragments arranged by size: 1498 1317 1255 907 599 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2811 3114 Size: 274 80 33 51 1443 249 585 303 Cuts at: 3114 4622 6334 96 Size: 1508 1712 138 Fragments arranged by size: 1712 1508 1443 585 303 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6336 6368 6336 Size: 32 6344 Tth111I GACn'n_nGTC Cuts at: 1316 3509 1316 Size: 2193 4183 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3363 3701 1870 Size: 1493 338 4545 Fragments arranged by size: 4545 1493 338 XmnI GAAnn'nnTTC Cuts at: 2547 2630 4505 2547 Size: 83 1875 4418 Fragments arranged by size: 4418 1875 83 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI