Restriction map for
pFN21AB8167
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BciVI BclI BfaI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BsrFI BsrGI
BssHII BssSI BstEII BstPI BstUI
BstXI BstYI CelII Cfr10I ClaI
CpoI DraI DraII DsaI EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 121 TCCGGA T^CCGGA
AcsI 175 GAATTT [GA]^AATT[CT]
AflII 595 CTTAAG C^TTAAG
AocI 217 CCTGAGG CC^T[ACGT]AGG
ApoI 175 GAATTT [GA]^AATT[CT]
Asp700I 172 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 464 GAGCAC G[AT]GC[AT]^C
BanI 1033 GGTGCC G^G[CT][GA]CC
BbvCI 697 GCTGAGG CCTCAGC(-5/-2)
BfrI 595 CTTAAG C^TTAAG
BsaWI 121 TCCGGA [AT]^CCGG[AT]
BseAI 121 TCCGGA T^CCGGA
BsiHKAI 464 GAGCAC G[AT]GC[AT]^C
BspEI 121 TCCGGA T^CCGGA
BspHI 20 TCATGA T^CATGA
BspMI 1134 GCAGGT ACCTGC(4/8)
BsrDI 68 GCAATG GCAATG(2/0)
Bst1107I 1063 GTATAC GTA^TAC
BstAPI 781 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 611 TTCGAA TT^CGAA
BstZ17I 1063 GTATAC GTA^TAC
Bsu36I 217 CCTGAGG CC^T[ACGT]AGG
DraIII 1177 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1151 GACGCTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1288 CGGCCA [CT]^GGCC[GA]
Eco81I 217 CCTGAGG CC^T[ACGT]AGG
EcoNI 927 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII 642 ACGT A^CGT
MroI 121 TCCGGA T^CCGGA
MspA1I 1262 CAGCTG C[AC]G^C[GT]G
NspI 917 GCATGC [GA]CATG^[CT]
NspV 611 TTCGAA TT^CGAA
PvuII 1262 CAGCTG CAG^CTG
RcaI 20 TCATGA T^CATGA
SfuI 611 TTCGAA TT^CGAA
SphI 917 GCATGC GCATG^C
XcmI 743 CCAAAAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 172 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 42 GTCTAC GT^[AC][GT]AC
1063 GTATAC
AlwNI 212 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
542 CAGAGTCTG
AvaII 6 GGTCC G^G[AT]CC
621 GGACC
BbsI 388 GAAGAC GAAGAC(2/6)
604 GTCTTC
BbvI 1261 GCAGC GCAGC(8/12)
778 GCTGC
BcnI 251 CCCGG CC^[GC]GG
1029 CCGGG
BmyI 464 GAGCAC G[AGT]GC[ACT]^C
1229 GTGCCC
BpuAI 388 GAAGAC GAAGAC(2/6)
604 GTCTTC
Bsp1286I 464 GAGCAC G[AGT]GC[ACT]^C
1229 GTGCCC
BsrBI 436 GAGCGG CCGCTC(-3/-3)
1285 GAGCGG
BstNI 774 CCTGG CC^[AT]GG
1092 CCTGG
Cac8I 462 GCGAGC GC[ACGT]^[ACGT]GC
917 GCATGC
EcoRII 774 CCTGG ^CC[AT]GG
1092 CCTGG
HgaI 1151 GACGC GACGC(5/10)
456 GCGTC
MslI 498 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1027 CACCGGGGTG
MvaI 774 CCTGG CC^[AT]GG
1092 CCTGG
MwoI 781 GCATTGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1154 GCTCAGGTCGC
NciI 251 CCCGG CC^[GC]GG
1029 CCGGG
TseI 778 GCTGC G^C[AT]GC
1261 GCAGC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI Aor51HI
BpmI BsaJI BseRI BsiYI BslI
BsmFI BsrI BssKI BstF5I CfoI
Cfr13I DdeI DpnII EarI Eco47III
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I