(Circular) (Six-base) MAPSORT of: pFN21AB8167.seq Check: 5947 from: 1 to: 6022 pFN21AB8167 6022 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2048 3069 3365 1937 Size: 111 1021 296 4594 Fragments arranged by size: 4594 1021 296 111 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2355 4085 5469 5737 1632 Size: 723 1730 1384 268 1917 Fragments arranged by size: 1917 1730 1384 723 268 AfeI AGC'GCT Cuts at: 2020 2892 3088 2020 Size: 872 196 4954 Fragments arranged by size: 4954 872 196 AflII C'TTAA_G Cuts at: 820 1017 2600 820 Size: 197 1583 4242 Fragments arranged by size: 4242 1583 197 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 3036 1166 Size: 1870 4152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2759 2791 2759 Size: 32 5990 AlwNI CAG_nnn'CTG Cuts at: 2222 2552 3765 5337 2222 Size: 330 1213 1572 2907 Fragments arranged by size: 2907 1572 1213 330 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 2180 3331 3557 3709 5818 1493 Size: 687 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 687 226 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 3347 1870 Size: 73 1404 4545 Fragments arranged by size: 4545 1404 73 BamHI G'GATC_C Cuts at: 3352 3719 3352 Size: 367 5655 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3038 3343 4798 618 Size: 325 217 627 1251 305 1455 1842 Fragments arranged by size: 1842 1455 1251 627 325 305 217 BanII G_rGCy'C Cuts at: 1391 3341 1391 Size: 1950 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 2602 928 Size: 971 501 202 4348 Fragments arranged by size: 4348 971 501 202 BbvCI CC'TCA_GC Cuts at: 2703 2703 Size: 6022 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4213 4247 1903 Size: 34 2276 34 3678 Fragments arranged by size: 3678 2276 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 3439 3439 Size: 6022 Bme1580I G_kGCm'C Cuts at: 1790 3238 4083 5239 1790 Size: 1448 845 1156 2573 Fragments arranged by size: 2573 1448 1156 845 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2060 3753 4711 Size: 416 53 83 399 24 643 1693 958 Cuts at: 4711 442 Size: 1753 Fragments arranged by size: 1753 1693 958 643 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2325 2371 2325 Size: 46 5976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2550 2699 3119 4682 1239 Size: 1311 149 420 1563 2579 Fragments arranged by size: 2579 1563 1311 420 149 Bpu10I CC'TnA_GC Cuts at: 2703 3160 2703 Size: 457 5565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2711 2915 4147 5012 5310 2711 Size: 204 1232 865 298 3423 Fragments arranged by size: 3423 1232 865 298 204 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4685 882 Size: 617 3186 2219 Fragments arranged by size: 3186 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 6016 6016 Size: 6022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3829 4211 4846 275 Size: 53 83 186 936 2296 382 635 1451 Fragments arranged by size: 2296 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2126 4455 4853 5127 5274 5685 2126 Size: 2329 398 274 147 411 2463 Fragments arranged by size: 2463 2329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5797 Fragments arranged by size: 5797 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2249 2426 2429 2549 2837 2962 1186 Size: 997 66 177 3 120 288 125 4246 Fragments arranged by size: 4246 997 288 177 125 120 66 3 BseYI C'CCAG_C Cuts at: 2054 3269 5225 5721 2054 Size: 1215 1956 496 2355 Fragments arranged by size: 2355 1956 1215 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5449 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3472 4233 5261 1540 Size: 275 186 1471 761 1028 2301 Fragments arranged by size: 2301 1471 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2473 4083 4168 5239 2473 Size: 1610 85 1071 3256 Fragments arranged by size: 3256 1610 1071 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2473 3238 3341 4083 4168 5239 1391 Size: 399 683 765 103 742 85 1071 2174 Fragments arranged by size: 2174 1071 765 742 683 399 103 85 BspEI T'CCGG_A Cuts at: 2126 5685 2126 Size: 3559 2463 BspHI T'CATG_A Cuts at: 2025 3911 5641 2025 Size: 1886 1730 2406 Fragments arranged by size: 2406 1886 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3130 3377 844 Size: 2286 247 3489 Fragments arranged by size: 3489 2286 247 BsrBI CCG'CTC Cuts at: 1394 1823 2443 3292 3909 1394 Size: 429 620 849 617 3507 Fragments arranged by size: 3507 849 620 617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2080 4517 4691 65 Size: 1183 832 2437 174 1396 Fragments arranged by size: 2437 1396 1183 832 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4666 4853 1700 Size: 110 2856 187 2869 Fragments arranged by size: 2869 2856 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6022 BssSI C'ACGA_G Cuts at: 4082 5094 4082 Size: 1012 5010 BstAPI GCAn_nnn'nTGC Cuts at: 794 2792 794 Size: 1998 4024 BstBI TT'CG_AA Cuts at: 2617 4826 2617 Size: 2209 3813 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstYI r'GATC_y Cuts at: 1972 3352 3719 4104 4121 5573 5659 5671 6017 Size: 1380 367 385 17 1452 86 12 346 Cuts at: 6017 1972 Size: 1977 Fragments arranged by size: 1977 1452 1380 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3070 3070 Size: 6022 Bsu36I CC'TnA_GG Cuts at: 2223 2223 Size: 6022 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5470 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5283 Fragments arranged by size: 5283 683 56 BtsI GCAGTG_nn' Cuts at: 2407 3538 4332 4352 2407 Size: 1131 794 20 4077 Fragments arranged by size: 4077 1131 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3190 3225 529 Size: 35 2626 35 3326 Fragments arranged by size: 3326 2626 35 35 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DraIII CAC_nnn'GTG Cuts at: 3187 3187 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3162 5029 809 Size: 507 1846 1867 1802 Fragments arranged by size: 1867 1846 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3293 3469 4358 Size: 54 1425 50 275 18 1463 176 889 Cuts at: 4358 5987 8 Size: 1629 43 Fragments arranged by size: 1629 1463 1425 889 275 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3469 1537 Size: 275 1657 4090 Fragments arranged by size: 4090 1657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2292 2403 2484 2556 2733 2757 2937 3952 Size: 939 111 81 72 177 24 180 1015 Cuts at: 3952 1353 Size: 3423 Fragments arranged by size: 3423 1015 939 180 177 111 81 72 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2355 2550 2699 3119 4085 4682 5469 Size: 393 723 195 149 420 966 597 787 Cuts at: 5469 5737 1239 Size: 268 1524 Fragments arranged by size: 1524 966 787 723 597 420 393 268 195 149 EcoNI CCTnn'n_nnAGG Cuts at: 2936 2936 Size: 6022 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2626 2626 Size: 6022 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 2022 2894 3090 5169 1678 Size: 344 872 196 2079 2531 Fragments arranged by size: 2531 2079 872 344 196 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 2528 2560 2578 Size: 32 135 32 656 32 268 32 18 Cuts at: 2578 2610 3188 3220 3281 3313 4663 4695 4737 Size: 32 578 32 61 32 1350 32 42 Cuts at: 4737 4769 1373 Size: 32 2626 Fragments arranged by size: 2626 1350 656 578 268 135 61 42 32 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5136 5320 1305 Size: 3831 184 2007 Fragments arranged by size: 3831 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4200 Fragments arranged by size: 4200 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2507 3036 3980 4339 4498 518 Size: 648 1341 529 944 359 159 2042 Fragments arranged by size: 2042 1341 944 648 529 359 159 MspA1I CmG'CkG Cuts at: 1606 3269 3438 4115 5263 5508 1606 Size: 1663 169 677 1148 245 2120 Fragments arranged by size: 2120 1663 1148 677 245 169 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5470 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 2926 3380 4925 1132 Size: 97 1697 454 1545 2229 Fragments arranged by size: 2229 1697 1545 454 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6022 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6022 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2622 2654 4091 4123 5604 5636 2622 Size: 32 1437 32 1481 32 3008 Fragments arranged by size: 3008 1481 1437 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PstI C_TGCA'G Cuts at: 830 3374 830 Size: 2544 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 PvuII CAG'CTG Cuts at: 3269 3269 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 3370 4505 5186 5377 826 Size: 219 2325 1135 681 191 1471 Fragments arranged by size: 2325 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 3349 1872 Size: 1477 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 2726 2894 4126 5027 5289 Size: 197 926 657 126 168 1232 901 262 Cuts at: 5289 820 Size: 1553 Fragments arranged by size: 1553 1232 926 901 657 262 197 168 126 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 2926 3380 1132 Size: 1794 454 3774 Fragments arranged by size: 3774 1794 454 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 3461 513 Size: 552 2396 3074 Fragments arranged by size: 3074 2396 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1999 2643 4049 4066 4219 4404 4849 Size: 1255 419 644 1406 17 153 185 445 Cuts at: 4849 325 Size: 1498 Fragments arranged by size: 1498 1406 1255 644 445 419 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2565 2718 4268 Size: 274 80 33 51 1443 588 153 1550 Cuts at: 4268 5980 96 Size: 1712 138 Fragments arranged by size: 1712 1550 1443 588 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5982 6014 5982 Size: 32 5990 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2755 2755 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 3347 1870 Size: 1477 4545 XmnI GAAnn'nnTTC Cuts at: 2181 4151 2181 Size: 1970 4052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI