Restriction map for
pFN21AB8167
(Linear) (Six-base) MAPSORT of: KIBB8167.seq Check: 2171 from: 1 to: 1317
KIBB8167.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 43 1064 1317
Size: 43 1021 253
Fragments arranged by size:
1021 253 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1317
Size: 350 967
AfeI AGC'GCT
Cuts at: 0 15 887 1083 1317
Size: 15 872 196 234
Fragments arranged by size:
872 234 196 15
AflII C'TTAA_G
Cuts at: 0 595 1317
Size: 595 722
AleI CACnn'nnGTG
Cuts at: 0 1031 1317
Size: 1031 286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 754 786 1317
Size: 754 32 531
Fragments arranged by size:
754 531 32
AlwNI CAG_nnn'CTG
Cuts at: 0 217 547 1317
Size: 217 330 770
Fragments arranged by size:
770 330 217
ApoI r'AATT_y
Cuts at: 0 175 1317
Size: 175 1142
BanI G'GyrC_C
Cuts at: 0 1033 1317
Size: 1033 284
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 597 1317
Size: 395 202 720
Fragments arranged by size:
720 395 202
BbvCI CC'TCA_GC
Cuts at: 0 698 1317
Size: 698 619
Bme1580I G_kGCm'C
Cuts at: 0 1233 1317
Size: 1233 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 1317
Size: 55 1262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 320 366 1317
Size: 320 46 951
Fragments arranged by size:
951 320 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 694 1114 1317
Size: 545 149 420 203
Fragments arranged by size:
545 420 203 149
Bpu10I CC'TnA_GC
Cuts at: 0 698 1155 1317
Size: 698 457 162
Fragments arranged by size:
698 457 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 910 1317
Size: 706 204 407
Fragments arranged by size:
706 407 204
BsaWI w'CCGG_w
Cuts at: 0 121 1317
Size: 121 1196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 244 421 424 544 832 957 1317
Size: 178 66 177 3 120 288 125 360
Fragments arranged by size:
360 288 178 177 125 120 66 3
BseYI C'CCAG_C
Cuts at: 0 49 1264 1317
Size: 49 1215 53
Fragments arranged by size:
1215 53 49
BsiHKAI G_wGCw'C
Cuts at: 0 468 1317
Size: 468 849
Bsp1286I G_dGCh'C
Cuts at: 0 468 1233 1317
Size: 468 765 84
Fragments arranged by size:
765 468 84
BspEI T'CCGG_A
Cuts at: 0 121 1317
Size: 121 1196
BspHI T'CATG_A
Cuts at: 0 20 1317
Size: 20 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1125 1317
Size: 1125 192
BsrBI CCG'CTC
Cuts at: 0 438 1287 1317
Size: 438 849 30
Fragments arranged by size:
849 438 30
BsrDI GCAATG_nn'
Cuts at: 0 75 1317
Size: 75 1242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 787 1317
Size: 787 530
BstBI TT'CG_AA
Cuts at: 0 612 1317
Size: 612 705
BstZ17I GTA'TAC
Cuts at: 0 1065 1317
Size: 1065 252
Bsu36I CC'TnA_GG
Cuts at: 0 218 1317
Size: 218 1099
BtsI GCAGTG_nn'
Cuts at: 0 402 1317
Size: 402 915
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1185 1220 1317
Size: 1185 35 97
Fragments arranged by size:
1185 97 35
DraIII CAC_nnn'GTG
Cuts at: 0 1182 1317
Size: 1182 135
DrdI GACnn_nn'nnGTC
Cuts at: 0 1157 1317
Size: 1157 160
EaeI y'GGCC_r
Cuts at: 0 1288 1317
Size: 1288 29
EarI CTCTTCn'nnn_
Cuts at: 0 287 398 479 551 728 752 932 1317
Size: 287 111 81 72 177 24 180 385
Fragments arranged by size:
385 287 180 177 111 81 72 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 545 694 1114 1317
Size: 350 195 149 420 203
Fragments arranged by size:
420 350 203 195 149
EcoNI CCTnn'n_nnAGG
Cuts at: 0 931 1317
Size: 931 386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 621 1317
Size: 621 696
HaeII r_GCGC'y
Cuts at: 0 17 889 1085 1317
Size: 17 872 196 232
Fragments arranged by size:
872 232 196 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 523 555 573 605 1183 1215
Size: 223 32 268 32 18 32 578 32
Cuts at: 1215 1276 1308 1317
Size: 61 32 9
Fragments arranged by size:
578 268 223 61 32 32 32 32
32 18 9
MslI CAynn'nnrTG
Cuts at: 0 502 1031 1317
Size: 502 529 286
Fragments arranged by size:
529 502 286
MspA1I CmG'CkG
Cuts at: 0 1264 1317
Size: 1264 53
NspI r_CATG'y
Cuts at: 0 921 1317
Size: 921 396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 617 649 1317
Size: 617 32 668
Fragments arranged by size:
668 617 32
PvuII CAG'CTG
Cuts at: 0 1264 1317
Size: 1264 53
SmlI C'TyrA_G
Cuts at: 0 595 721 889 1317
Size: 595 126 168 428
Fragments arranged by size:
595 428 168 126
SphI G_CATG'C
Cuts at: 0 921 1317
Size: 921 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 638 1317
Size: 638 679
TatI w'GTAC_w
Cuts at: 0 560 713 1317
Size: 560 153 604
Fragments arranged by size:
604 560 153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 1317
Size: 750 567
XmnI GAAnn'nnTTC
Cuts at: 0 176 1317
Size: 176 1141
Enzymes that do cut:
AccI AcuI AfeI AflII AleI AloI AlwNI ApoI
BanI BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BstAPI BstBI BstZ17I Bsu36I BtsI
CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI FalI
HaeII Hin4I MslI MspA1I NspI PpiI PvuII SmlI
SphI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaHI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstYI BtgI BtgZI ClaI
DraI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI