Restriction map for
pFN21AB8166
(Circular) (Six-base) MAPSORT of: pFN21AB8166.seq Check: 1149 from: 1 to: 6637
pFN21AB8166 6637 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3680 3680
Size: 6637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4447 278
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3117 3792 3980 1937
Size: 1180 675 188 4594
Fragments arranged by size:
4594 1180 675 188
Acc65I G'GTAC_C
Cuts at: 2595 3958 2595
Size: 1363 5274
AclI AA'CG_TT
Cuts at: 1563 4764 5137 1563
Size: 3201 373 3063
Fragments arranged by size:
3201 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2538 3378 4700 6084 6352 1632
Size: 461 445 840 1322 1384 268 1917
Fragments arranged by size:
1917 1384 1322 840 461 445 268
AflII C'TTAA_G
Cuts at: 820 1017 3203 820
Size: 197 2186 4254
Fragments arranged by size:
4254 2186 197
AflIII A'CryG_T
Cuts at: 1203 5536 6513 1203
Size: 4333 977 1327
Fragments arranged by size:
4333 1327 977
AgeI A'CCGG_T
Cuts at: 5468 5468
Size: 6637
AhdI GACnn_n'nnGTC
Cuts at: 5366 5366
Size: 6637
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3688 3899 1184
Size: 2504 211 3922
Fragments arranged by size:
3922 2504 211
AlwNI CAG_nnn'CTG
Cuts at: 3283 3518 3854 4380 5952 3283
Size: 235 336 526 1572 3968
Fragments arranged by size:
3968 1572 526 336 235
ApaLI G'TGCA_C
Cuts at: 4694 5850 4694
Size: 1156 5481
ApoI r'AATT_y
Cuts at: 1493 2306 2348 2607 3946 4172 4324 6433 1493
Size: 813 42 259 1339 226 152 2109 1697
Fragments arranged by size:
2109 1697 1339 813 259 226 152 42
AseI AT'TA_AT
Cuts at: 160 1032 5191 160
Size: 872 4159 1606
Fragments arranged by size:
4159 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 3423 3962 1870
Size: 73 1480 539 4545
Fragments arranged by size:
4545 1480 539 73
BamHI G'GATC_C
Cuts at: 2392 3967 4334 2392
Size: 1575 367 4695
Fragments arranged by size:
4695 1575 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2595 3778 3958 5413 618
Size: 325 217 627 808 1183 180 1455 1842
Fragments arranged by size:
1842 1455 1183 808 627 325 217 180
BanII G_rGCy'C
Cuts at: 1391 2896 3149 3956 1391
Size: 1505 253 807 4072
Fragments arranged by size:
4072 1505 807 253
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 2340 3204 3543 928
Size: 971 144 297 864 339 4022
Fragments arranged by size:
4022 971 864 339 297 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2616 2650 4828 4862 1903
Size: 34 679 34 2178 34 3678
Fragments arranged by size:
3678 2178 679 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2849 3727 4529 5739 2849
Size: 878 802 1210 3747
Fragments arranged by size:
3747 1210 878 802
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2845 5248 136
Size: 107 122 71 631 605 1173 2403 1525
Fragments arranged by size:
2403 1525 1173 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2931 3253 6632 2931
Size: 322 3379 2936
Fragments arranged by size:
3379 2936 322
BlpI GC'TnA_GC
Cuts at: 4054 4054
Size: 6637
Bme1580I G_kGCm'C
Cuts at: 1790 4698 5854 1790
Size: 2908 1156 2573
Fragments arranged by size:
2908 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2878 4368 5326
Size: 416 53 83 399 24 1461 1490 958
Cuts at: 5326 442
Size: 1753
Fragments arranged by size:
1753 1490 1461 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 5297 1239
Size: 1248 2810 2579
Fragments arranged by size:
2810 2579 1248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3614 4762 5627 5925 3614
Size: 1148 865 298 4326
Fragments arranged by size:
4326 1148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2712 5300 882
Size: 617 1213 2588 2219
Fragments arranged by size:
2588 2219 1213 617
BsaAI yAC'GTr
Cuts at: 493 1204 4428 493
Size: 711 3224 2702
Fragments arranged by size:
3224 2702 711
BsaBI GATnn'nnATC
Cuts at: 6631 6631
Size: 6637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4444 4826 5461 275
Size: 53 83 186 936 2911 382 635 1451
Fragments arranged by size:
2911 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5070 5468 5742 5889 6300 5070
Size: 398 274 147 411 5407
Fragments arranged by size:
5407 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3756 3786 1178
Size: 30 165 30 2353 30 4029
Fragments arranged by size:
4029 2353 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2859 3344 1186
Size: 1673 485 4479
Fragments arranged by size:
4479 1673 485
BseYI C'CCAG_C
Cuts at: 5840 6336 5840
Size: 496 6141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2436 3905 1163
Size: 573 700 1469 3895
Fragments arranged by size:
3895 1469 700 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4087 4848 5876 1540
Size: 275 186 2086 761 1028 2301
Fragments arranged by size:
2301 2086 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2632 4698 4783 5854 2632
Size: 2066 85 1071 3415
Fragments arranged by size:
3415 2066 1071 85
BsmI GAATG_Cn'
Cuts at: 4148 4241 4148
Size: 93 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2632 2896 3149 3956 4698 4783 5854
Size: 399 842 264 253 807 742 85 1071
Cuts at: 5854 1391
Size: 2174
Fragments arranged by size:
2174 1071 842 807 742 399 264 253
85
BspEI T'CCGG_A
Cuts at: 6300 6300
Size: 6637
BspHI T'CATG_A
Cuts at: 4526 6256 4526
Size: 1730 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2739 3680 3862 3992 844
Size: 1895 941 182 130 3489
Fragments arranged by size:
3489 1895 941 182 130
BsrBI CCG'CTC
Cuts at: 1394 1823 4524 1394
Size: 429 2701 3507
Fragments arranged by size:
3507 2701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5132 5306 65
Size: 1183 3884 174 1396
Fragments arranged by size:
3884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2844 5281 5468 1700
Size: 110 1034 2437 187 2869
Fragments arranged by size:
2869 2437 1034 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6637
BssSI C'ACGA_G
Cuts at: 4697 5709 4697
Size: 1012 5625
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3854 794
Size: 3060 3577
BstBI TT'CG_AA
Cuts at: 5441 5441
Size: 6637
BstEII G'GTnAC_C
Cuts at: 1184 2481 1184
Size: 1297 5340
BstXI CCAn_nnnn'nTGG
Cuts at: 2686 2686
Size: 6637
BstYI r'GATC_y
Cuts at: 1972 2392 2931 3253 3967 4334 4719 4736 6188
Size: 420 539 322 714 367 385 17 1452
Cuts at: 6188 6274 6286 6632 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 714 539 420 385 367 346
322 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2428 513
Size: 552 939 424 4722
Fragments arranged by size:
4722 939 552 424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5898
Fragments arranged by size:
5898 683 56
BtsI GCAGTG_nn'
Cuts at: 2632 2667 4153 4947 4967 2632
Size: 35 1486 794 20 4302
Fragments arranged by size:
4302 1486 794 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2496 2531 529
Size: 35 1932 35 4635
Fragments arranged by size:
4635 1932 35 35
DraI TTT'AAA
Cuts at: 3941 3941
Size: 6637
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5644 809
Size: 507 4328 1802
Fragments arranged by size:
4328 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3122 4084 4973
Size: 54 1425 50 275 18 1292 962 889
Cuts at: 4973 6602 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1292 962 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4084 1537
Size: 275 2272 4090
Fragments arranged by size:
4090 2272 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3591 4567 1353
Size: 2238 976 3423
Fragments arranged by size:
3423 2238 976
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2863 5224 5598 5744 387
Size: 1064 138 1274 2361 374 146 1280
Fragments arranged by size:
2361 1280 1274 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2487 2538 3378 4700 5297 6084
Size: 393 461 394 51 840 1322 597 787
Cuts at: 6084 6352 1239
Size: 268 1524
Fragments arranged by size:
1524 1322 840 787 597 461 394 393
268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2205 2205
Size: 6637
EcoO109I rG'GnC_Cy
Cuts at: 3851 3851
Size: 6637
EcoRI G'AATT_C
Cuts at: 2348 3946 4324 2348
Size: 1598 378 4661
Fragments arranged by size:
4661 1598 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2123 2776 3635 2123
Size: 653 859 5125
Fragments arranged by size:
5125 859 653
FspI TGC'GCA
Cuts at: 5143 5143
Size: 6637
HaeII r_GCGC'y
Cuts at: 1678 2677 5784 1678
Size: 999 3107 2531
Fragments arranged by size:
3107 2531 999
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2916 2948 5278 5310 5352
Size: 32 135 32 1344 32 2330 32 42
Cuts at: 5352 5384 1373
Size: 32 2626
Fragments arranged by size:
2626 2330 1344 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3981 4227 669
Size: 988 281 2043 246 3079
Fragments arranged by size:
3079 2043 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2021 3378 748
Size: 1273 1357 4007
Fragments arranged by size:
4007 1357 1273
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KpnI G_GTAC'C
Cuts at: 2599 3962 2599
Size: 1363 5274
MfeI C'AATT_G
Cuts at: 2816 4236 2816
Size: 1420 5217
MluI A'CGCG_T
Cuts at: 6513 6513
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5751 5935 1305
Size: 4446 184 2007
Fragments arranged by size:
4446 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3124 10
Size: 54 1425 343 1292 3523
Fragments arranged by size:
3523 1425 1292 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3881 4595 4954 5113 518
Size: 648 2715 714 359 159 2042
Fragments arranged by size:
2715 2042 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2648 3271 3280 3889 4053 4730 5878 6123
Size: 1042 623 9 609 164 677 1148 245
Cuts at: 6123 1606
Size: 2120
Fragments arranged by size:
2120 1148 1042 677 623 609 245 164
9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2428 513
Size: 552 939 424 4722
Fragments arranged by size:
4722 939 552 424
NdeI CA'TA_TG
Cuts at: 387 2226 387
Size: 1839 4798
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 4084 4084
Size: 6637
NspI r_CATG'y
Cuts at: 1132 1229 3995 5540 1132
Size: 97 2766 1545 2229
Fragments arranged by size:
2766 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6637
PciI A'CATG_T
Cuts at: 5536 5536
Size: 6637
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 1108
Size: 1794 4843
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4738 6219 6251 4706
Size: 32 1481 32 5092
Fragments arranged by size:
5092 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3851 3851
Size: 6637
PshAI GACnn'nnGTC
Cuts at: 1613 2786 1613
Size: 1173 5464
PsiI TTA'TAA
Cuts at: 4207 4207
Size: 6637
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2042 2074 2042
Size: 32 6605
PstI C_TGCA'G
Cuts at: 830 2695 3288 3989 830
Size: 1865 593 701 3478
Fragments arranged by size:
3478 1865 701 593
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
PvuII CAG'CTG
Cuts at: 2648 3271 3280 3889 2648
Size: 623 9 609 5396
Fragments arranged by size:
5396 623 609 9
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SbfI CC_TGCA'GG
Cuts at: 3989 3989
Size: 6637
ScaI AGT'ACT
Cuts at: 2051 4885 6597 2051
Size: 2834 1712 2091
Fragments arranged by size:
2834 2091 1712
SexAI A'CCwGG_T
Cuts at: 3801 3801
Size: 6637
SfcI C'TryA_G
Cuts at: 826 1045 2691 3284 3826 3985 5120 5801 5992
Size: 219 1646 593 542 159 1135 681 191
Cuts at: 5992 826
Size: 1471
Fragments arranged by size:
1646 1471 1135 681 593 542 219 191
159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6637
SmaI CCC'GGG
Cuts at: 1872 3964 1872
Size: 2092 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3203 3423 3629 4741 5642 5904
Size: 197 926 1260 220 206 1112 901 262
Cuts at: 5904 820
Size: 1553
Fragments arranged by size:
1553 1260 1112 926 901 262 220 206
197
SnaBI TAC'GTA
Cuts at: 493 4428 493
Size: 3935 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6637
SphI G_CATG'C
Cuts at: 1132 3995 1132
Size: 2863 3774
SspI AAT'ATT
Cuts at: 5 52 4561 5
Size: 47 4509 2081
Fragments arranged by size:
4509 2081 47
StuI AGG'CCT
Cuts at: 1517 2615 3502 1517
Size: 1098 887 4652
Fragments arranged by size:
4652 1098 887
StyI C'CwwG_G
Cuts at: 513 1065 2004 2428 3324 3497 4076 513
Size: 552 939 424 896 173 579 3074
Fragments arranged by size:
3074 939 896 579 552 424 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 4664 4681 4834 5019 5464 325
Size: 1255 1196 1888 17 153 185 445 1498
Fragments arranged by size:
1888 1498 1255 1196 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 2115 4883
Size: 274 80 33 51 1443 72 66 2768
Cuts at: 4883 6595 96
Size: 1712 138
Fragments arranged by size:
2768 1712 1443 274 138 80 72 66
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2197 2229 2622 2654 6597 6629 2197
Size: 32 393 32 3943 32 2205
Fragments arranged by size:
3943 2205 393 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3973 3973
Size: 6637
XhoI C'TCGA_G
Cuts at: 1943 3423 1943
Size: 1480 5157
XmaI C'CCGG_G
Cuts at: 1870 3962 1870
Size: 2092 4545
XmnI GAAnn'nnTTC
Cuts at: 2607 3101 3380 4766 2607
Size: 494 279 1386 4478
Fragments arranged by size:
4478 1386 494 279
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4445 276
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI