Restriction map for
pFN21AB8165
(Circular) (Six-base) MAPSORT of: pFN21AB8165.seq Check: 9011 from: 1 to: 6193
pFN21AB8165 6193 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2541 3536 1937
Size: 604 995 4594
Fragments arranged by size:
4594 995 604
Acc65I G'GTAC_C
Cuts at: 2525 2982 3514 2525
Size: 457 532 5204
Fragments arranged by size:
5204 532 457
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 2765 4256 5640 5908 1632
Size: 612 521 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 612 521 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2150 3036 5092 6069 1203
Size: 947 886 2056 977 1327
Fragments arranged by size:
2056 1327 977 947 886
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 6161
AlwNI CAG_nnn'CTG
Cuts at: 2755 2878 3936 5508 2755
Size: 123 1058 1572 3440
Fragments arranged by size:
3440 1572 1058 123
ApaI G_GGCC'C
Cuts at: 2189 2189
Size: 6193
ApaLI G'TGCA_C
Cuts at: 2213 4250 5406 2213
Size: 2037 1156 3000
Fragments arranged by size:
3000 2037 1156
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2928 3518 1870
Size: 73 985 590 4545
Fragments arranged by size:
4545 985 590 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2966 2999 2966
Size: 33 6160
BamHI G'GATC_C
Cuts at: 3360 3523 3890 3360
Size: 163 367 5663
Fragments arranged by size:
5663 367 163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2525 2614 2982 3514
Size: 325 217 627 294 444 89 368 532
Cuts at: 3514 4969 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 532 444 368 325 294
217 89
BanII G_rGCy'C
Cuts at: 1391 2189 2904 3098 3512 1391
Size: 798 715 194 414 4072
Fragments arranged by size:
4072 798 715 414 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3429 928
Size: 971 1530 3692
Fragments arranged by size:
3692 1530 971
BbvCI CC'TCA_GC
Cuts at: 2438 2438
Size: 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2328 6188 2328
Size: 3860 2333
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2189 2217 2617 4254 5410 1790
Size: 296 103 28 400 1637 1156 2573
Fragments arranged by size:
2573 1637 1156 400 296 103 28
BmgBI CAC'GTC
Cuts at: 1318 2268 3159 1318
Size: 950 891 4352
Fragments arranged by size:
4352 950 891
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2525 3924 4882
Size: 416 53 83 399 24 1108 1399 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1399 1108 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2274 2417 2510 4853 1239
Size: 918 117 143 93 2343 2579
Fragments arranged by size:
2579 2343 918 143 117 93
Bpu10I CC'TnA_GC
Cuts at: 2088 2438 2088
Size: 350 5843
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2633 2849 2888 4318 5183 5481 2567
Size: 66 216 39 1430 865 298 3279
Fragments arranged by size:
3279 1430 865 298 216 66 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2486 2853 4856 882
Size: 617 987 367 2003 2219
Fragments arranged by size:
2219 2003 987 617 367
BsaAI yAC'GTr
Cuts at: 493 1204 2153 3984 493
Size: 711 949 1831 2702
Fragments arranged by size:
2702 1831 949 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4000 4382 5017 275
Size: 53 83 186 936 2467 382 635 1451
Fragments arranged by size:
2467 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3167 3197 3260 1186
Size: 1981 30 63 4119
Fragments arranged by size:
4119 1981 63 30
BseYI C'CCAG_C
Cuts at: 5396 5892 5396
Size: 496 5697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2163 2184 2303 2605 1163
Size: 573 427 21 119 302 4751
Fragments arranged by size:
4751 573 427 302 119 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2904 4254 4339 5410 2217
Size: 687 1350 85 1071 3000
Fragments arranged by size:
3000 1350 1071 687 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
BsmBI CGTCTCn'nnnn_
Cuts at: 3149 3149
Size: 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2189 2217 2617 2904 3098 3512
Size: 399 296 103 28 400 287 194 414
Cuts at: 3512 4254 4339 5410 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 414 400 399 296 287
194 103 85 28
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2886 3548 844
Size: 2042 662 3489
Fragments arranged by size:
3489 2042 662
BsrBI CCG'CTC
Cuts at: 1394 1823 3358 4080 1394
Size: 429 1535 722 3507
Fragments arranged by size:
3507 1535 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2838 2985 4837 5024 1700
Size: 110 1028 147 1852 187 2869
Fragments arranged by size:
2869 1852 1028 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 2216 4253 5265 2216
Size: 2037 1012 3144
Fragments arranged by size:
3144 2037 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2848 3320 794
Size: 2054 472 3667
Fragments arranged by size:
3667 2054 472
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 2968 2968
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 2328 3306 3360 3523 3890 4275 4292 5744
Size: 356 978 54 163 367 385 17 1452
Cuts at: 5744 5830 5842 6188 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 978 385 367 356 346 163
86 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2385 2385
Size: 6193
BtgI C'CryG_G
Cuts at: 513 1065 2004 2818 3012 3099 513
Size: 552 939 814 194 87 3607
Fragments arranged by size:
3607 939 814 552 194 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2134 3709 4503 4523 2134
Size: 1575 794 20 3804
Fragments arranged by size:
3804 1575 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3013 3048 529
Size: 35 2449 35 3674
Fragments arranged by size:
3674 2449 35 35
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DraIII CAC_nnn'GTG
Cuts at: 2791 2791
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 3069 3161
Size: 54 1425 50 275 18 226 1013 92
Cuts at: 3161 3640 4529 6158 8
Size: 479 889 1629 43
Fragments arranged by size:
1629 1425 1013 889 479 275 226 92
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2601 4123 1353
Size: 1248 1522 3423
Fragments arranged by size:
3423 1522 1248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2902 2902
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2244 2274 2417 2510 2765 4256
Size: 393 525 87 30 143 93 255 1491
Cuts at: 4256 4853 5640 5908 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1491 787 597 525 393 268 255
143 93 87 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2648 2735 3453 2648
Size: 87 718 5388
Fragments arranged by size:
5388 718 87
EcoO109I rG'GnC_Cy
Cuts at: 2143 2185 2653 2740 3223 2143
Size: 42 468 87 483 5113
Fragments arranged by size:
5113 483 468 87 42
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 2309 4699 2309
Size: 2390 3803
HaeII r_GCGC'y
Cuts at: 1678 2356 5340 1678
Size: 678 2984 2531
Fragments arranged by size:
2984 2531 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 4834 4866 4908
Size: 32 135 32 698 32 2532 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2626 2532 698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 2529 2986 3518 2529
Size: 457 532 5204
Fragments arranged by size:
5204 532 457
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5491 1305
Size: 4002 184 2007
Fragments arranged by size:
4002 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3071 3163 10
Size: 54 1425 343 1239 92 3040
Fragments arranged by size:
3040 1425 1239 343 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2780 4151 4510 4669 518
Size: 648 1614 1371 359 159 2042
Fragments arranged by size:
2042 1614 1371 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2369 2812 3609 4286 5434 5679 1606
Size: 429 334 443 797 677 1148 245 2120
Fragments arranged by size:
2120 1148 797 677 443 429 334 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 2785 3040 3551 5096 1132
Size: 97 1556 255 511 1545 2229
Fragments arranged by size:
2229 1556 1545 511 255 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 3036 5092 3036
Size: 2056 4137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PfoI T'CCnGG_A
Cuts at: 2631 2631
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PmlI CAC'GTG
Cuts at: 2153 2153
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 2740 2653
Size: 87 6106
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 2185 2185
Size: 6193
PstI C_TGCA'G
Cuts at: 830 3545 830
Size: 2715 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2035 2369 2812 2035
Size: 334 443 5416
Fragments arranged by size:
5416 443 334
SacI G_AGCT'C
Cuts at: 2904 2904
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 3541 4676 5357 5548 826
Size: 219 2496 1135 681 191 1471
Fragments arranged by size:
2496 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2582 2648 2864 2903 4297 5198
Size: 197 926 639 66 216 39 1394 901
Cuts at: 5198 5460 820
Size: 262 1553
Fragments arranged by size:
1553 1394 926 901 639 262 216 197
66 39
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 2100 1517
Size: 583 5610
StyI C'CwwG_G
Cuts at: 513 1065 2004 3632 513
Size: 552 939 1628 3074
Fragments arranged by size:
3074 1628 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3423 4220 4237 4390 4575 5020 325
Size: 1255 1843 797 17 153 185 445 1498
Fragments arranged by size:
1843 1498 1255 797 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4439 6151 96
Size: 274 80 33 51 1443 2462 1712 138
Fragments arranged by size:
2462 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3064 3096 6153 6185 3064
Size: 32 3057 32 3072
Fragments arranged by size:
3072 3057 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2816 2816
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 2798 4322 2798
Size: 1524 4669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI