Restriction map for
pFN21AB8163
(Circular) (Six-base) MAPSORT of: pFN21AB8163.seq Check: 8539 from: 1 to: 5074
pFN21AB8163 5074 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2884 278
Size: 53 83 186 936 1348 2468
Fragments arranged by size:
2468 1348 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2417 1937
Size: 480 4594
Acc65I G'GTAC_C
Cuts at: 2395 2395
Size: 5074
AclI AA'CG_TT
Cuts at: 1563 3201 3574 1563
Size: 1638 373 3063
Fragments arranged by size:
3063 1638 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3137 4521 4789 1632
Size: 1505 1384 268 1917
Fragments arranged by size:
1917 1505 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4877
AflIII A'CryG_T
Cuts at: 1203 2223 3973 4950 1203
Size: 1020 1750 977 1327
Fragments arranged by size:
1750 1327 1020 977
AgeI A'CCGG_T
Cuts at: 3905 3905
Size: 5074
AhdI GACnn_n'nnGTC
Cuts at: 3803 3803
Size: 5074
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5074
AlwNI CAG_nnn'CTG
Cuts at: 2817 4389 2817
Size: 1572 3502
ApaLI G'TGCA_C
Cuts at: 3131 4287 3131
Size: 1156 3918
ApoI r'AATT_y
Cuts at: 1493 2383 2609 2761 4870 1493
Size: 890 226 152 2109 1697
Fragments arranged by size:
2109 1697 890 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3628 160
Size: 872 2596 1606
Fragments arranged by size:
2596 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5074
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2399 1870
Size: 73 145 311 4545
Fragments arranged by size:
4545 311 145 73
BamHI G'GATC_C
Cuts at: 2404 2771 2404
Size: 367 4707
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2395 3850 618
Size: 325 217 627 608 1455 1842
Fragments arranged by size:
1842 1455 627 608 325 217
BanII G_rGCy'C
Cuts at: 1391 2393 1391
Size: 1002 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3265 3299 1903
Size: 34 1328 34 3678
Fragments arranged by size:
3678 1328 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2966 4176 2966
Size: 1210 3864
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5074
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3685 136
Size: 107 122 71 631 605 2013 1525
Fragments arranged by size:
2013 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5069 5069
Size: 5074
BlpI GC'TnA_GC
Cuts at: 2491 2491
Size: 5074
Bme1580I G_kGCm'C
Cuts at: 1790 3135 4291 1790
Size: 1345 1156 2573
Fragments arranged by size:
2573 1345 1156
BmgBI CAC'GTC
Cuts at: 1318 2260 1318
Size: 942 4132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2805 3763 442
Size: 416 53 83 399 24 1388 958 1753
Fragments arranged by size:
1753 1388 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 3734 1239
Size: 944 1551 2579
Fragments arranged by size:
2579 1551 944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3199 4064 4362 3199
Size: 865 298 3911
Fragments arranged by size:
3911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3737 882
Size: 617 2238 2219
Fragments arranged by size:
2238 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2865 493
Size: 711 1661 2702
Fragments arranged by size:
2702 1661 711
BsaBI GATnn'nnATC
Cuts at: 5068 5068
Size: 5074
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2881 3263 3898 275
Size: 53 83 186 936 1348 382 635 1451
Fragments arranged by size:
1451 1348 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 3507 3905 4179 4326 4737 2055
Size: 1452 398 274 147 411 2392
Fragments arranged by size:
2392 1452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4849
Fragments arranged by size:
4849 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 1186
Size: 1000 4074
BseYI C'CCAG_C
Cuts at: 4277 4773 4277
Size: 496 4578
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4501
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2524 3285 4313 1540
Size: 275 186 523 761 1028 2301
Fragments arranged by size:
2301 1028 761 523 275 186
BsiHKAI G_wGCw'C
Cuts at: 3135 3220 4291 3135
Size: 85 1071 3918
Fragments arranged by size:
3918 1071 85
BsmI GAATG_Cn'
Cuts at: 2585 2678 2585
Size: 93 4981
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2393 3135 3220 4291 1391
Size: 399 603 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 85
BspEI T'CCGG_A
Cuts at: 4737 4737
Size: 5074
BspHI T'CATG_A
Cuts at: 2963 4693 2963
Size: 1730 3344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2429 844
Size: 1585 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2111 2961 1394
Size: 429 288 850 3507
Fragments arranged by size:
3507 850 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 3569 3743 65
Size: 1183 1069 1252 174 1396
Fragments arranged by size:
1396 1252 1183 1069 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3718 3905 1700
Size: 110 1908 187 2869
Fragments arranged by size:
2869 1908 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5074
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5074
BssSI C'ACGA_G
Cuts at: 3134 4146 3134
Size: 1012 4062
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5074
BstBI TT'CG_AA
Cuts at: 3878 3878
Size: 5074
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5074
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 2313 2260
Size: 53 5021
BstYI r'GATC_y
Cuts at: 1972 2404 2771 3156 3173 4625 4711 4723 5069
Size: 432 367 385 17 1452 86 12 346
Cuts at: 5069 1972
Size: 1977
Fragments arranged by size:
1977 1452 432 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3583
Fragments arranged by size:
3583 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4335
Fragments arranged by size:
4335 683 56
BtsI GCAGTG_nn'
Cuts at: 2590 3384 3404 2590
Size: 794 20 4260
Fragments arranged by size:
4260 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5074
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5039
DraI TTT'AAA
Cuts at: 2378 2378
Size: 5074
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4081 809
Size: 507 2765 1802
Fragments arranged by size:
2765 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2521 3410 5039
Size: 54 1425 50 275 18 691 889 1629
Cuts at: 5039 8
Size: 43
Fragments arranged by size:
1629 1425 889 691 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2521 1537
Size: 275 709 4090
Fragments arranged by size:
4090 709 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 3004 1353
Size: 767 884 3423
Fragments arranged by size:
3423 884 767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3661 4035 4181 387
Size: 1064 138 2072 374 146 1280
Fragments arranged by size:
2072 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 3137 3734 4521 4789 1239
Size: 393 551 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 597 551 393 268
EcoRI G'AATT_C
Cuts at: 2383 2761 2383
Size: 378 4696
FspI TGC'GCA
Cuts at: 2287 3580 2287
Size: 1293 3781
HaeII r_GCGC'y
Cuts at: 1678 4221 1678
Size: 2543 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 3715 3747 3789
Size: 32 135 32 449 32 1662 32 42
Cuts at: 3789 3821 1373
Size: 32 2626
Fragments arranged by size:
2626 1662 449 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2418 2664 669
Size: 988 281 480 246 3079
Fragments arranged by size:
3079 988 480 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5074
HpaI GTT'AAC
Cuts at: 2664 2664
Size: 5074
KpnI G_GTAC'C
Cuts at: 2399 2399
Size: 5074
MfeI C'AATT_G
Cuts at: 2307 2673 2307
Size: 366 4708
MluI A'CGCG_T
Cuts at: 4950 4950
Size: 5074
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4188 4372 1305
Size: 2883 184 2007
Fragments arranged by size:
2883 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3252
Fragments arranged by size:
3252 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3032 3391 3550 518
Size: 648 1866 359 159 2042
Fragments arranged by size:
2042 1866 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2490 3167 4315 4560 1606
Size: 409 475 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 475 409 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3583
Fragments arranged by size:
3583 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5074
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5074
NotI GC'GGCC_GC
Cuts at: 2521 2521
Size: 5074
NspI r_CATG'y
Cuts at: 1132 1229 2227 2432 3977 1132
Size: 97 998 205 1545 2229
Fragments arranged by size:
2229 1545 998 205 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5074
PciI A'CATG_T
Cuts at: 2223 3973 2223
Size: 1750 3324
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5074
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 5074
PmeI GTTT'AAAC
Cuts at: 2378 2378
Size: 5074
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2210 2242 3143 3175 4656 4688 2210
Size: 32 901 32 1481 32 2596
Fragments arranged by size:
2596 1481 901 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5074
PsiI TTA'TAA
Cuts at: 2644 2644
Size: 5074
PstI C_TGCA'G
Cuts at: 830 2100 2426 830
Size: 1270 326 3478
Fragments arranged by size:
3478 1270 326
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5074
SalI G'TCGA_C
Cuts at: 1936 2416 1936
Size: 480 4594
SbfI CC_TGCA'GG
Cuts at: 2426 2426
Size: 5074
ScaI AGT'ACT
Cuts at: 3322 5034 3322
Size: 1712 3362
SfcI C'TryA_G
Cuts at: 826 1045 2096 2422 3557 4238 4429 826
Size: 219 1051 326 1135 681 191 1471
Fragments arranged by size:
1471 1135 1051 681 326 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5074
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5074
SmaI CCC'GGG
Cuts at: 1872 2090 2401 1872
Size: 218 311 4545
Fragments arranged by size:
4545 311 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3178 4079 4341 820
Size: 197 926 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2865 493
Size: 2372 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5074
SphI G_CATG'C
Cuts at: 1132 2432 1132
Size: 1300 3774
SspI AAT'ATT
Cuts at: 5 52 2998 5
Size: 47 2946 2081
Fragments arranged by size:
2946 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5074
StyI C'CwwG_G
Cuts at: 513 1065 2004 2253 2352 2513 513
Size: 552 939 249 99 161 3074
Fragments arranged by size:
3074 939 552 249 161 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3101 3118 3271 3456 3901 325
Size: 1255 1521 17 153 185 445 1498
Fragments arranged by size:
1521 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3320 5032 96
Size: 274 80 33 51 1443 1343 1712 138
Fragments arranged by size:
1712 1443 1343 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5034 5066 5034
Size: 32 5042
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5074
XbaI T'CTAG_A
Cuts at: 2410 2410
Size: 5074
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5074
XmaI C'CCGG_G
Cuts at: 1870 2088 2399 1870
Size: 218 311 4545
Fragments arranged by size:
4545 311 218
XmnI GAAnn'nnTTC
Cuts at: 2339 3203 2339
Size: 864 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2882 276
Size: 53 83 186 936 1348 2468
Fragments arranged by size:
2468 1348 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI