(Circular) (Six-base) MAPSORT of: pFN21AB8160.seq Check: 5914 from: 1 to: 5485 pFN21AB8160 5485 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3548 4932 5200 1632 Size: 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 2310 4384 5361 1203 Size: 1107 2074 977 1327 Fragments arranged by size: 2074 1327 1107 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 2417 1166 Size: 1251 4234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 2174 Size: 32 5453 AlwNI CAG_nnn'CTG Cuts at: 2154 2428 3228 4800 2154 Size: 274 800 1572 2839 Fragments arranged by size: 2839 1572 800 274 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2085 2188 2777 2794 3020 3172 5281 1493 Size: 592 103 589 17 226 152 2109 1697 Fragments arranged by size: 2109 1697 592 589 226 152 103 17 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2810 1870 Size: 73 867 4545 Fragments arranged by size: 4545 867 73 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2273 2653 2806 4261 618 Size: 325 217 627 486 380 153 1455 1842 Fragments arranged by size: 1842 1455 627 486 380 325 217 153 BanII G_rGCy'C Cuts at: 1391 2052 2148 2804 1391 Size: 661 96 656 4072 Fragments arranged by size: 4072 661 656 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2313 928 Size: 971 414 4100 Fragments arranged by size: 4100 971 414 BbvCI CC'TCA_GC Cuts at: 2714 2714 Size: 5485 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BfrBI ATG'CAT Cuts at: 2649 2649 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 4096 136 Size: 107 122 71 631 605 583 1841 1525 Fragments arranged by size: 1841 1525 631 605 583 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2147 2902 2147 Size: 755 4730 Bme1580I G_kGCm'C Cuts at: 1790 2478 2670 2766 3546 4702 1790 Size: 688 192 96 780 1156 2573 Fragments arranged by size: 2573 1156 780 688 192 96 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2755 3216 4174 Size: 416 53 83 399 24 1338 461 958 Cuts at: 4174 442 Size: 1753 Fragments arranged by size: 1753 1338 958 461 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2002 2066 2505 4145 1239 Size: 763 64 439 1640 2579 Fragments arranged by size: 2579 1640 763 439 64 Bpu10I CC'TnA_GC Cuts at: 2714 2714 Size: 5485 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3610 4475 4773 3610 Size: 865 298 4322 Fragments arranged by size: 4322 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4148 882 Size: 617 2649 2219 Fragments arranged by size: 2649 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3292 3674 4309 275 Size: 53 83 186 936 1759 382 635 1451 Fragments arranged by size: 1759 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5260 Fragments arranged by size: 5260 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2337 1186 Size: 1151 4334 BseYI C'CCAG_C Cuts at: 4688 5184 4688 Size: 496 4989 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 2441 1163 Size: 573 579 126 4207 Fragments arranged by size: 4207 579 573 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3696 4724 1540 Size: 275 186 934 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 2148 3546 3631 4702 2052 Size: 96 1398 85 1071 2835 Fragments arranged by size: 2835 1398 1071 96 85 BsmI GAATG_Cn' Cuts at: 2300 2651 2996 3089 2300 Size: 351 345 93 4696 Fragments arranged by size: 4696 351 345 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2148 2478 2670 2766 2804 3546 Size: 399 262 96 330 192 96 38 742 Cuts at: 3546 3631 4702 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 330 262 192 96 96 85 38 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 2302 3374 5104 2302 Size: 1072 1730 2683 Fragments arranged by size: 2683 1730 1072 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2614 2840 844 Size: 1770 226 3489 Fragments arranged by size: 3489 1770 226 BsrBI CCG'CTC Cuts at: 1394 1823 3372 1394 Size: 429 1549 3507 Fragments arranged by size: 3507 1549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2466 2478 3980 4154 65 Size: 1183 1218 12 1502 174 1396 Fragments arranged by size: 1502 1396 1218 1183 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2656 4129 4316 1700 Size: 110 846 1473 187 2869 Fragments arranged by size: 2869 1473 846 187 110 BsrGI T'GTAC_A Cuts at: 96 2754 96 Size: 2658 2827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 2669 3545 4557 2669 Size: 876 1012 3597 Fragments arranged by size: 3597 1012 876 BstAPI GCAn_nnn'nTGC Cuts at: 794 2572 794 Size: 1778 3707 BstBI TT'CG_AA Cuts at: 2533 4289 2533 Size: 1756 3729 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2727 2815 3182 3567 3584 5036 5122 5134 Size: 755 88 367 385 17 1452 86 12 Cuts at: 5134 5480 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 755 385 367 346 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2365 2522 2365 Size: 157 5328 BtgI C'CryG_G Cuts at: 513 1065 2004 2058 2203 513 Size: 552 939 54 145 3795 Fragments arranged by size: 3795 939 552 145 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 3001 3795 3815 3001 Size: 794 20 4671 Fragments arranged by size: 4671 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2932 3821 Size: 54 1425 50 275 18 231 871 889 Cuts at: 3821 5450 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 871 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3415 1353 Size: 2062 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4072 4446 4592 387 Size: 1064 138 2483 374 146 1280 Fragments arranged by size: 2483 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2050 2146 2050 Size: 96 5389 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2066 2505 3548 4145 4932 5200 Size: 393 370 64 439 1043 597 787 268 Cuts at: 5200 1239 Size: 1524 Fragments arranged by size: 1524 1043 787 597 439 393 370 268 64 EcoRI G'AATT_C Cuts at: 2777 2794 3172 2777 Size: 17 378 5090 Fragments arranged by size: 5090 378 17 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 5485 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 2154 4632 1678 Size: 476 2478 2531 Fragments arranged by size: 2531 2478 476 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2327 2359 4126 Size: 32 135 32 428 32 295 32 1767 Cuts at: 4126 4158 4200 4232 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1767 428 295 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2829 3075 669 Size: 988 281 891 246 3079 Fragments arranged by size: 3079 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4599 4783 1305 Size: 3294 184 2007 Fragments arranged by size: 3294 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3663 Fragments arranged by size: 3663 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 2293 2417 2471 3443 3802 3961 Size: 648 897 230 124 54 972 359 159 Cuts at: 3961 518 Size: 2042 Fragments arranged by size: 2042 972 897 648 359 230 159 124 54 MspA1I CmG'CkG Cuts at: 1606 2044 2462 2563 2901 3578 4726 4971 1606 Size: 438 418 101 338 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 438 418 338 245 101 NcoI C'CATG_G Cuts at: 513 1065 2004 2058 2203 513 Size: 552 939 54 145 3795 Fragments arranged by size: 3795 939 552 145 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NsiI A_TGCA'T Cuts at: 2651 2651 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2314 2755 2843 4388 1132 Size: 97 1085 441 88 1545 2229 Fragments arranged by size: 2229 1545 1085 441 97 88 PasI CC'CwG_GG Cuts at: 1348 2352 1348 Size: 1004 4481 PciI A'CATG_T Cuts at: 2310 4384 2310 Size: 2074 3411 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5485 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2435 2467 3554 3586 5067 5099 2435 Size: 32 1087 32 1481 32 2821 Fragments arranged by size: 2821 1481 1087 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PstI C_TGCA'G Cuts at: 830 2043 2556 2837 830 Size: 1213 513 281 3478 Fragments arranged by size: 3478 1213 513 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 PvuII CAG'CTG Cuts at: 2044 2044 Size: 5485 SacI G_AGCT'C Cuts at: 2052 2148 2052 Size: 96 5389 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2039 2552 2590 2833 3968 4649 4840 Size: 219 994 513 38 243 1135 681 191 Cuts at: 4840 826 Size: 1471 Fragments arranged by size: 1471 1135 994 681 513 243 219 191 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3589 4490 4752 820 Size: 197 926 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 2363 1517 Size: 846 4639 StyI C'CwwG_G Cuts at: 513 1065 2004 2058 2203 2415 2924 513 Size: 552 939 54 145 212 509 3074 Fragments arranged by size: 3074 939 552 509 212 145 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2216 3512 3529 3682 3867 4312 325 Size: 1255 636 1296 17 153 185 445 1498 Fragments arranged by size: 1498 1296 1255 636 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 3731 5443 Size: 274 80 33 51 1443 777 977 1712 Cuts at: 5443 96 Size: 138 Fragments arranged by size: 1712 1443 977 777 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5445 5477 5445 Size: 32 5453 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2162 2821 2162 Size: 659 4826 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 2287 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI