Restriction map for
pFN21AB8158
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AccII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI Ksp632I KspI MamI MflI
MluI MluNI MroI MscI MseI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 923 GACGTC GACGT^C
Acc65I 425 GGTACC G^GTACC
Aor51HI 110 AGCGCT AGC^GCT
ApaI 27 GGGCCC GGGCC^C
Asp700I 990 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 425 GGTACC G^GTACC
AspHI 1036 GTGCTC G[AT]GC[AT]^C
AvaI 1031 CTCGGG C^[CT]CG[GA]G
BbeI 74 GGCGCC GGCGC^C
BbsI 388 GTCTTC GAAGAC(2/6)
BciVI 1008 GGATAC GTATCC(6/5)
BpuAI 388 GTCTTC GAAGAC(2/6)
BsgI 819 GTGCAG GTGCAG(16/14)
BsiHKAI 1036 GTGCTC G[AT]GC[AT]^C
BsmFI 656 GGGAC GGGAC(10/14)
BsoBI 1031 CTCGGG C^[CT]CG[GA]G
BspHI 617 TCATGA T^CATGA
BstXI 1062 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 656 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1062 CCATGG C^C[GA][CT]GG
Eco47III 110 AGCGCT AGC^GCT
EcoO109I 656 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 448 GATATC GAT^ATC
FauI 35 CCCGC CCCGC(4/6)
KasI 74 GGCGCC G^GCGCC
KpnI 425 GGTACC GGTAC^C
MfeI 567 CAATTG C^AATTG
MslI 1063 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 567 CAATTG C^AATTG
NarI 74 GGCGCC GG^CGCC
NcoI 1062 CCATGG C^CATGG
NspI 861 GCATGC [GA]CATG^[CT]
PpuMI 656 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 617 TCATGA T^CATGA
SfoI 74 GGCGCC GGC^GCC
SphI 861 GCATGC GCATG^C
Tsp509I 568 AATT ^AATT
XcmI 337 CCACGACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 990 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcyI 74 GGCGCC G[GA]^CG[CT]C
923 GACGTC
AlwNI 513 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1112 CAGCCTCTG
AvaII 252 GGTCC G^G[AT]CC
657 GGACC
BanII 27 GGGCCC G[GA]GC[CT]^C
1084 GAGCCC
BsaHI 74 GGCGCC G[GA]^CG[CT]C
923 GACGTC
BsmBI 459 CGTCTC CGTCTC(1/5)
921 GAGACG
BstAPI 125 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
512 GCAGCATCTGC
BstF5I 315 CATCC GGATG(2/0)
782 GGATG
DdeI 330 CTCAG C^T[ACGT]AG
1110 CTCAG
EcoT14I 938 CCAAGG C^C[AT][AT]GG
1062 CCATGG
FokI 315 CATCC GGATG(9/13)
782 GGATG
Hin1I 74 GGCGCC G[GA]^CG[CT]C
923 GACGTC
MaeII 221 ACGT A^CGT
924 ACGT
PstI 510 CTGCAG CTGCA^G
639 CTGCAG
StyI 938 CCAAGG C^C[AT][AT]GG
1062 CCATGG
TfiI 372 GAATC G^A[AT]TC
957 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
BanI BbvI BcnI BfaI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I