Restriction map for
pFN21AB8158
(Linear) (Six-base) MAPSORT of: KIBB8158.seq Check: 5657 from: 1 to: 1131
KIBB8158.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 927 1131
Size: 927 204
AccI GT'mk_AC
Cuts at: 0 203 215 635 803 1131
Size: 203 12 420 168 328
Fragments arranged by size:
420 328 203 168 12
Acc65I G'GTAC_C
Cuts at: 0 425 1131
Size: 425 706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1131
Size: 556 575
AfeI AGC'GCT
Cuts at: 0 112 1131
Size: 112 1019
AlwNI CAG_nnn'CTG
Cuts at: 0 518 1117 1131
Size: 518 599 14
Fragments arranged by size:
599 518 14
ApaI G_GGCC'C
Cuts at: 0 31 1131
Size: 31 1100
AvaI C'yCGr_G
Cuts at: 0 1031 1131
Size: 1031 100
BanI G'GyrC_C
Cuts at: 0 74 425 844 1131
Size: 74 351 419 287
Fragments arranged by size:
419 351 287 74
BanII G_rGCy'C
Cuts at: 0 31 1088 1131
Size: 31 1057 43
Fragments arranged by size:
1057 43 31
BbeI G_GCGC'C
Cuts at: 0 78 1131
Size: 78 1053
BbsI GAAGACnn'nnnn_
Cuts at: 0 381 1131
Size: 381 750
BciVI GTATCCnnnnn_n'
Cuts at: 0 1002 1131
Size: 1002 129
Bme1580I G_kGCm'C
Cuts at: 0 31 849 1131
Size: 31 818 282
Fragments arranged by size:
818 282 31
BsaHI Gr'CG_yC
Cuts at: 0 75 924 1131
Size: 75 849 207
Fragments arranged by size:
849 207 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 77 107 1131
Size: 77 30 1024
Fragments arranged by size:
1024 77 30
BseYI C'CCAG_C
Cuts at: 0 24 63 1079 1131
Size: 24 39 1016 52
Fragments arranged by size:
1016 52 39 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1131
Size: 840 291
BsiHKAI G_wGCw'C
Cuts at: 0 1040 1131
Size: 1040 91
BsmBI CGTCTCn'nnnn_
Cuts at: 0 465 915 1131
Size: 465 450 216
Fragments arranged by size:
465 450 216
Bsp1286I G_dGCh'C
Cuts at: 0 31 849 1040 1088 1131
Size: 31 818 191 48 43
Fragments arranged by size:
818 191 48 43 31
BspHI T'CATG_A
Cuts at: 0 617 1131
Size: 617 514
BstAPI GCAn_nnn'nTGC
Cuts at: 0 131 518 1131
Size: 131 387 613
Fragments arranged by size:
613 387 131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1069 1131
Size: 1069 62
BtgI C'CryG_G
Cuts at: 0 1062 1131
Size: 1062 69
BtsI GCAGTG_nn'
Cuts at: 0 174 507 549 1131
Size: 174 333 42 582
Fragments arranged by size:
582 333 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 407 1131
Size: 37 370 724
Fragments arranged by size:
724 370 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1131
Size: 556 575
EcoO109I rG'GnC_Cy
Cuts at: 0 657 1131
Size: 657 474
EcoRV GAT'ATC
Cuts at: 0 450 1131
Size: 450 681
HaeII r_GCGC'y
Cuts at: 0 78 114 120 1131
Size: 78 36 6 1011
Fragments arranged by size:
1011 78 36 6
KasI G'GCGC_C
Cuts at: 0 74 1131
Size: 74 1057
KpnI G_GTAC'C
Cuts at: 0 429 1131
Size: 429 702
MfeI C'AATT_G
Cuts at: 0 567 1131
Size: 567 564
MslI CAynn'nnrTG
Cuts at: 0 1067 1131
Size: 1067 64
MspA1I CmG'CkG
Cuts at: 0 12 80 178 230 1131
Size: 12 68 98 52 901
Fragments arranged by size:
901 98 68 52 12
NarI GG'CG_CC
Cuts at: 0 75 1131
Size: 75 1056
NcoI C'CATG_G
Cuts at: 0 1062 1131
Size: 1062 69
NspI r_CATG'y
Cuts at: 0 865 1131
Size: 865 266
PasI CC'CwG_GG
Cuts at: 0 435 840 1094 1131
Size: 435 405 254 37
Fragments arranged by size:
435 405 254 37
PfoI T'CCnGG_A
Cuts at: 0 665 1131
Size: 665 466
PpuMI rG'GwC_Cy
Cuts at: 0 657 1131
Size: 657 474
PspOMI G'GGCC_C
Cuts at: 0 27 1131
Size: 27 1104
PstI C_TGCA'G
Cuts at: 0 514 643 1131
Size: 514 129 488
Fragments arranged by size:
514 488 129
SfcI C'TryA_G
Cuts at: 0 360 510 639 718 931 1131
Size: 360 150 129 79 213 200
Fragments arranged by size:
360 213 200 150 129 79
SfoI GGC'GCC
Cuts at: 0 76 1131
Size: 76 1055
SphI G_CATG'C
Cuts at: 0 865 1131
Size: 865 266
StyI C'CwwG_G
Cuts at: 0 938 1062 1131
Size: 938 124 69
Fragments arranged by size:
938 124 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 774 832 991 1086 1131
Size: 774 58 159 95 45
Fragments arranged by size:
774 159 95 58 45
TatI w'GTAC_w
Cuts at: 0 105 1131
Size: 105 1026
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 77 109 1131
Size: 77 32 1022
Fragments arranged by size:
1022 77 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 344 1131
Size: 344 787
XmnI GAAnn'nnTTC
Cuts at: 0 994 1131
Size: 994 137
ZraI GAC'GTC
Cuts at: 0 925 1131
Size: 925 206
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BciVI Bme1580I BsaHI BsaXI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BstAPI BstXI
BtgI BtsI EciI Eco57MI EcoO109I EcoRV HaeII KasI
KpnI MfeI MslI MspA1I NarI NcoI NspI PasI
PfoI PpuMI PspOMI PstI SfcI SfoI SphI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI