(Circular) (Six-base) MAPSORT of: pFN21AB8157.seq Check: 6038 from: 1 to: 6352 pFN21AB8157 6352 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3267 3581 3695 1937 Size: 1330 314 114 4594 Fragments arranged by size: 4594 1330 314 114 Acc65I G'GTAC_C Cuts at: 2551 3673 2551 Size: 1122 5230 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3619 4415 5799 6067 1632 Size: 1987 796 1384 268 1917 Fragments arranged by size: 1987 1917 1384 796 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6155 AflIII A'CryG_T Cuts at: 1203 5251 6228 1203 Size: 4048 977 1327 Fragments arranged by size: 4048 1327 977 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AlwNI CAG_nnn'CTG Cuts at: 4095 5667 4095 Size: 1572 4780 ApaI G_GGCC'C Cuts at: 3341 3341 Size: 6352 ApaLI G'TGCA_C Cuts at: 4409 5565 4409 Size: 1156 5196 ApoI r'AATT_y Cuts at: 1493 2377 2563 3082 3092 3661 3887 4039 6148 Size: 884 186 519 10 569 226 152 2109 Cuts at: 6148 1493 Size: 1697 Fragments arranged by size: 2109 1697 884 569 519 226 186 152 10 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3677 1870 Size: 73 1734 4545 Fragments arranged by size: 4545 1734 73 AvrII C'CTAG_G Cuts at: 2135 2135 Size: 6352 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2551 3191 3673 5128 618 Size: 325 217 627 764 640 482 1455 1842 Fragments arranged by size: 1842 1455 764 640 627 482 325 217 BanII G_rGCy'C Cuts at: 1391 3341 3671 1391 Size: 1950 330 4072 Fragments arranged by size: 4072 1950 330 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3363 928 Size: 971 1464 3917 Fragments arranged by size: 3917 1464 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4543 4577 1903 Size: 34 2606 34 3678 Fragments arranged by size: 3678 2606 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2866 4244 5454 2866 Size: 1378 1210 3764 Fragments arranged by size: 3764 1378 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4963 136 Size: 107 122 71 631 605 3291 1525 Fragments arranged by size: 3291 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2545 6347 2545 Size: 3802 2550 BlpI GC'TnA_GC Cuts at: 2196 3769 2196 Size: 1573 4779 Bme1580I G_kGCm'C Cuts at: 1790 3341 4413 5569 1790 Size: 1551 1072 1156 2573 Fragments arranged by size: 2573 1551 1156 1072 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4083 5041 442 Size: 416 53 83 399 24 2666 958 1753 Fragments arranged by size: 2666 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5012 1239 Size: 3773 2579 Bpu10I CC'TnA_GC Cuts at: 2201 3514 2201 Size: 1313 5039 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4477 5342 5640 4477 Size: 865 298 5189 Fragments arranged by size: 5189 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5015 882 Size: 617 3516 2219 Fragments arranged by size: 3516 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4143 493 Size: 711 2939 2702 Fragments arranged by size: 2939 2702 711 BsaBI GATnn'nnATC Cuts at: 2909 6346 2909 Size: 3437 2915 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4159 4541 5176 275 Size: 53 83 186 936 2626 382 635 1451 Fragments arranged by size: 2626 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3374 4785 5183 5457 5604 6015 3374 Size: 1411 398 274 147 411 3711 Fragments arranged by size: 3711 1411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3223 3253 3536 3566 1178 Size: 30 165 30 1820 30 283 30 3964 Fragments arranged by size: 3964 1820 283 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2944 3225 3228 3464 3572 1186 Size: 1758 281 3 236 108 3966 Fragments arranged by size: 3966 1758 281 236 108 3 BseYI C'CCAG_C Cuts at: 5555 6051 5555 Size: 496 5856 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5779 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3802 4563 5591 1540 Size: 275 186 1801 761 1028 2301 Fragments arranged by size: 2301 1801 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2167 4413 4498 5569 2167 Size: 2246 85 1071 2950 Fragments arranged by size: 2950 2246 1071 85 BsmI GAATG_Cn' Cuts at: 2683 3863 3956 2683 Size: 1180 93 5079 Fragments arranged by size: 5079 1180 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2167 3341 3671 4413 4498 5569 1391 Size: 399 377 1174 330 742 85 1071 2174 Fragments arranged by size: 2174 1174 1071 742 399 377 330 85 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 4241 5971 4241 Size: 1730 4622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 3560 3707 844 Size: 1210 1506 147 3489 Fragments arranged by size: 3489 1506 1210 147 BsrBI CCG'CTC Cuts at: 1394 1823 4239 1394 Size: 429 2416 3507 Fragments arranged by size: 3507 2416 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4847 5021 65 Size: 1183 3599 174 1396 Fragments arranged by size: 3599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4996 5183 1700 Size: 110 3186 187 2869 Fragments arranged by size: 3186 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6352 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 2046 794 Size: 1252 5100 BstBI TT'CG_AA Cuts at: 5156 5156 Size: 6352 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstXI CCAn_nnnn'nTGG Cuts at: 3137 3137 Size: 6352 BstYI r'GATC_y Cuts at: 1972 2458 2545 3682 4049 4434 4451 5903 5989 Size: 486 87 1137 367 385 17 1452 86 Cuts at: 5989 6001 6347 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1137 486 385 367 346 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2481 2481 Size: 6352 BtgI C'CryG_G Cuts at: 513 1065 2859 513 Size: 552 1794 4006 Fragments arranged by size: 4006 1794 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5613 Fragments arranged by size: 5613 683 56 BtsI GCAGTG_nn' Cuts at: 3868 4662 4682 3868 Size: 794 20 5538 Fragments arranged by size: 5538 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6317 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3348 3799 4688 Size: 54 1425 50 275 18 1518 451 889 Cuts at: 4688 6317 8 Size: 1629 43 Fragments arranged by size: 1629 1518 1425 889 451 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 2484 3112 3528 3609 4282 1353 Size: 834 297 628 416 81 673 3423 Fragments arranged by size: 3423 834 673 628 416 297 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3619 4415 5012 5799 6067 1239 Size: 393 1987 796 597 787 268 1524 Fragments arranged by size: 1987 1524 796 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3029 3029 Size: 6352 EcoO109I rG'GnC_Cy Cuts at: 2719 3337 3338 2719 Size: 618 1 5733 Fragments arranged by size: 5733 618 1 EcoRI G'AATT_C Cuts at: 2377 3082 3661 4039 2377 Size: 705 579 378 4690 Fragments arranged by size: 4690 705 579 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 6352 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 5499 1678 Size: 3821 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3223 3255 3536 3568 4993 Size: 32 135 32 1651 32 281 32 1425 Cuts at: 4993 5025 5067 5099 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1651 1425 281 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3575 3696 3942 669 Size: 988 281 1637 121 246 3079 Fragments arranged by size: 3079 1637 988 281 246 121 HindIII A'AGCT_T Cuts at: 748 748 Size: 6352 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KpnI G_GTAC'C Cuts at: 2555 3677 2555 Size: 1122 5230 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2427 2755 5466 5650 1305 Size: 1122 328 2711 184 2007 Fragments arranged by size: 2711 2007 1122 328 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3350 10 Size: 54 1425 343 1518 3012 Fragments arranged by size: 3012 1518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4310 4669 4828 518 Size: 648 3144 359 159 2042 Fragments arranged by size: 3144 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3768 4445 5593 5838 1606 Size: 2162 677 1148 245 2120 Fragments arranged by size: 2162 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2859 513 Size: 552 1794 4006 Fragments arranged by size: 4006 1794 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2881 3523 3710 5255 1132 Size: 97 1652 642 187 1545 2229 Fragments arranged by size: 2229 1652 1545 642 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6352 PciI A'CATG_T Cuts at: 5251 5251 Size: 6352 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6352 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 3922 3922 Size: 6352 PspOMI G'GGCC_C Cuts at: 3337 3337 Size: 6352 PstI C_TGCA'G Cuts at: 830 3532 3704 830 Size: 2702 172 3478 Fragments arranged by size: 3478 2702 172 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SapI GCTCTTCn'nnn_ Cuts at: 2187 2187 Size: 6352 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 3054 4600 6312 3054 Size: 1546 1712 3094 Fragments arranged by size: 3094 1712 1546 SfcI C'TryA_G Cuts at: 826 1045 3076 3528 3564 3700 4835 5516 5707 Size: 219 2031 452 36 136 1135 681 191 Cuts at: 5707 826 Size: 1471 Fragments arranged by size: 2031 1471 1135 681 452 219 191 136 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4456 5357 5619 820 Size: 197 926 2513 901 262 1553 Fragments arranged by size: 2513 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 2881 3710 1132 Size: 1749 829 3774 Fragments arranged by size: 3774 1749 829 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 2014 1517 Size: 497 5855 StyI C'CwwG_G Cuts at: 513 1065 2135 2859 2892 3791 513 Size: 552 1070 724 33 899 3074 Fragments arranged by size: 3074 1070 899 724 552 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4379 4396 4549 4734 5179 325 Size: 1255 2799 17 153 185 445 1498 Fragments arranged by size: 2799 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 3052 3638 Size: 274 80 33 51 1443 414 661 586 Cuts at: 3638 4598 6310 96 Size: 960 1712 138 Fragments arranged by size: 1712 1443 960 661 586 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6312 6344 6312 Size: 32 6320 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 4481 4481 Size: 6352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI