Restriction map for
pFN21AB8156
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# NO CUT ENZYMES ( 145 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BfaI BfrI BglI BlnI BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FokI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI MaeI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1003 GACGTC GACGT^C
AccII 6 CGCG CG^CG
AcyI 1003 GACGTC G[GA]^CG[CT]C
AfaI 792 GTAC GT^AC
Alw44I 145 GTGCAC G^TGCAC
AlwNI 319 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 145 GTGCAC G^TGCAC
BbvCI 1015 GCTGAGG CCTCAGC(-5/-2)
BclI 504 TGATCA T^GATCA
BcnI 986 CCCGG CC^[GC]GG
BlpI 808 GCTGAGC GC^T[ACGT]AGC
BpmI 599 CTGGAG CTGGAG(16/14)
Bpu1102I 808 GCTGAGC GC^T[ACGT]AGC
BsaHI 1003 GACGTC G[GA]^CG[CT]C
BsaJI 5 CCGCGG C^C[ACGT][ACGT]GG
BsiYI 795 CCTATTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 795 CCTATTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 273 ACCTGC ACCTGC(4/8)
BstUI 6 CGCG CG^CG
CelII 808 GCTGAGC GC^T[ACGT]AGC
CfoI 112 GCGC GCG^C
DsaI 5 CCGCGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoNI 795 CCTATTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 504 TGATCA T^GATCA
HapII 987 CCGG C^CGG
HgaI 932 GACGC GACGC(5/10)
HhaI 112 GCGC GCG^C
Hin1I 1003 GACGTC G[GA]^CG[CT]C
HinP1I 112 GCGC G^CGC
HpaII 987 CCGG C^CGG
KspI 5 CCGCGG CCGC^GG
MaeIII 193 GTTAC ^GT[ACGT]AC
MslI 709 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 987 CCGG C^CGG
MvnI 6 CGCG CG^CG
NciI 986 CCCGG CC^[GC]GG
NlaIV 9 GGTTCC GG[ACGT]^[ACGT]CC
PaeR7I 305 CTCGAG C^TCGAG
PstI 22 CTGCAG CTGCA^G
RsaI 792 GTAC GT^AC
SacI 877 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
SapI 769 GAAGAGC GCTCTTC(1/4)
SfcI 22 CTGCAG C^T[GA][CT]AG
SmlI 305 CTCGAG C^T[CT][GA]AG
XhoI 305 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Asp700I 430 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
636 GAAGAACTTC
AspHI 145 GTGCAC G[AT]GC[AT]^C
877 GAGCTC
AspI 127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACTGTGTC
AvaI 13 CCCGAG C^[CT]CG[GA]G
305 CTCGAG
BbsI 208 GAAGAC GAAGAC(2/6)
411 GAAGAC
BglII 55 AGATCT A^GATCT
703 AGATCT
BpuAI 208 GAAGAC GAAGAC(2/6)
411 GAAGAC
BsaBI 56 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
505 GATCAGGATC
BseRI 880 CTCCTC GAGGAG(10/8)
34 GAGGAG
BsiHKAI 145 GTGCAC G[AT]GC[AT]^C
877 GAGCTC
BsmAI 125 GAGAC GTCTC(1/5)
1171 GTCTC
BsoBI 13 CCCGAG C^[CT]CG[GA]G
305 CTCGAG
Cac8I 47 GCAAGC GC[ACGT]^[ACGT]GC
745 GCCTGC
EarI 769 GAAGAG CTCTTC(1/4)
1098 GAAGAG
HaeIII 3 GGCC GG^CC
984 GGCC
Ksp632I 769 GAAGAG CTCTTC(1/4)
1098 GAAGAG
MaeII 169 ACGT A^CGT
1004 ACGT
MamI 56 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
505 GATCAGGATC
MspA1I 5 CCGCGG C[AC]G^C[GT]G
442 CAGCGG
NspI 893 ACATGC [GA]CATG^[CT]
1155 ACATGC
PflFI 127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACTGTGTC
SfaNI 109 GATGC GCATC(5/9)
315 GATGC
TaqI 306 TCGA T^CGA
843 TCGA
Tth111I 127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACTGTGTC
TthHB8I 306 TCGA T^CGA
843 TCGA
XmnI 430 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
636 GAAGAACTTC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanII BbvI BmyI Bsp1286I
BsrI BssKI BstNI BstYI Cfr13I
DdeI DpnII EcoRII Fnu4HI HinfI
HphI ItaI MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp509I TspRI XhoII