(Circular) (Six-base) MAPSORT of: pFN21AB8156.seq Check: 5255 from: 1 to: 5917 pFN21AB8156 5917 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2287 2287 Size: 5917 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3012 3727 278 Size: 53 83 186 936 1476 715 2468 Fragments arranged by size: 2468 1476 936 715 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 3216 4044 4417 1563 Size: 1653 828 373 3063 Fragments arranged by size: 3063 1653 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2429 3980 5364 5632 1632 Size: 449 348 1551 1384 268 1917 Fragments arranged by size: 1917 1551 1384 449 348 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 4816 5793 1203 Size: 3613 977 1327 Fragments arranged by size: 3613 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3161 3193 3161 Size: 32 5885 AlwNI CAG_nnn'CTG Cuts at: 2329 3660 5232 2329 Size: 1331 1572 3014 Fragments arranged by size: 3014 1572 1331 ApaLI G'TGCA_C Cuts at: 2150 3974 5130 2150 Size: 1824 1156 2937 Fragments arranged by size: 2937 1824 1156 ApoI r'AATT_y Cuts at: 1493 2141 2363 3050 3091 3226 3452 3604 5713 Size: 648 222 687 41 135 226 152 2109 Cuts at: 5713 1493 Size: 1697 Fragments arranged by size: 2109 1697 687 648 226 222 152 135 41 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2018 2310 3242 1870 Size: 73 75 292 932 4545 Fragments arranged by size: 4545 932 292 75 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3238 4693 618 Size: 325 217 627 1451 1455 1842 Fragments arranged by size: 1842 1455 1451 627 325 217 BanII G_rGCy'C Cuts at: 1391 2186 2781 2886 3236 1391 Size: 795 595 105 350 4072 Fragments arranged by size: 4072 795 595 350 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2220 2423 928 Size: 971 321 203 4422 Fragments arranged by size: 4422 971 321 203 BbvCI CC'TCA_GC Cuts at: 3021 3021 Size: 5917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 2509 1548 Size: 961 4956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2060 2708 5912 2060 Size: 648 3204 2065 Fragments arranged by size: 3204 2065 648 BlpI GC'TnA_GC Cuts at: 2814 3334 2814 Size: 520 5397 Bme1580I G_kGCm'C Cuts at: 1790 2154 3978 5134 1790 Size: 364 1824 1156 2573 Fragments arranged by size: 2573 1824 1156 364 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2339 3648 4606 Size: 416 53 83 399 24 922 1309 958 Cuts at: 4606 442 Size: 1753 Fragments arranged by size: 1753 1309 958 922 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2625 4577 1239 Size: 1386 1952 2579 Fragments arranged by size: 2579 1952 1386 Bpu10I CC'TnA_GC Cuts at: 2919 3021 2919 Size: 102 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4042 4907 5205 4042 Size: 865 298 4754 Fragments arranged by size: 4754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4580 882 Size: 617 3081 2219 Fragments arranged by size: 3081 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 2065 2514 5911 2065 Size: 449 3397 2071 Fragments arranged by size: 3397 2071 449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3009 3724 4106 4741 Size: 53 83 186 936 1476 715 382 635 Cuts at: 4741 275 Size: 1451 Fragments arranged by size: 1476 1451 936 715 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3161 3191 1178 Size: 30 165 30 1758 30 3904 Fragments arranged by size: 3904 1758 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2876 1186 Size: 868 822 4227 Fragments arranged by size: 4227 868 822 BseYI C'CCAG_C Cuts at: 2261 5120 5616 2261 Size: 2859 496 2562 Fragments arranged by size: 2859 2562 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 2886 3978 4063 5134 2154 Size: 732 1092 85 1071 2937 Fragments arranged by size: 2937 1092 1071 732 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2186 2781 2886 3236 3978 4063 Size: 399 364 32 595 105 350 742 85 Cuts at: 4063 5134 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 595 399 364 350 105 85 32 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2287 3272 844 Size: 1443 985 3489 Fragments arranged by size: 3489 1443 985 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2060 2515 2708 2923 3247 3614 3999 4016 Size: 88 455 193 215 324 367 385 17 Cuts at: 4016 5468 5554 5566 5912 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 455 385 367 346 324 215 193 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 513 Size: 552 939 6 4420 Fragments arranged by size: 4420 939 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2165 3433 4227 4247 2165 Size: 1268 794 20 3835 Fragments arranged by size: 3835 1268 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3364 4253 Size: 54 1425 50 275 18 177 1357 889 Cuts at: 4253 5882 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1357 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2769 3098 3847 1353 Size: 1416 329 749 3423 Fragments arranged by size: 3423 1416 749 329 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2545 4504 4878 5024 387 Size: 1064 138 956 1959 374 146 1280 Fragments arranged by size: 1959 1280 1064 956 374 146 138 EcoICRI GAG'CTC Cuts at: 2884 2884 Size: 5917 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2429 2625 3980 4577 5364 5632 Size: 393 449 348 196 1355 597 787 268 Cuts at: 5632 1239 Size: 1524 Fragments arranged by size: 1524 1355 787 597 449 393 348 268 196 EcoNI CCTnn'n_nnAGG Cuts at: 2804 2804 Size: 5917 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2314 2459 2944 2314 Size: 145 485 5287 Fragments arranged by size: 5287 485 145 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 5064 1678 Size: 3386 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 2695 2727 4558 Size: 32 135 32 549 32 542 32 1831 Cuts at: 4558 4590 4632 4664 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1831 549 542 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5031 5215 1305 Size: 3726 184 2007 Fragments arranged by size: 3726 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4095 Fragments arranged by size: 4095 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2718 3875 4234 4393 518 Size: 648 1552 1157 359 159 2042 Fragments arranged by size: 2042 1552 1157 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2449 3333 4010 5158 5403 1606 Size: 406 437 884 677 1148 245 2120 Fragments arranged by size: 2120 1148 884 677 437 406 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4426 Fragments arranged by size: 4426 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 2902 3164 3275 4820 1132 Size: 97 1673 262 111 1545 2229 Fragments arranged by size: 2229 1673 1545 262 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5917 PfoI T'CCnGG_A Cuts at: 2499 2499 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3161 3193 3986 4018 5499 5531 3161 Size: 32 793 32 1481 32 3547 Fragments arranged by size: 3547 1481 793 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PstI C_TGCA'G Cuts at: 830 2031 3269 830 Size: 1201 1238 3478 Fragments arranged by size: 3478 1238 1201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 SacI G_AGCT'C Cuts at: 2886 2886 Size: 5917 SacII CC_GC'GG Cuts at: 2013 2013 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SapI GCTCTTCn'nnn_ Cuts at: 2769 2769 Size: 5917 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 4165 5877 4165 Size: 1712 4205 SfcI C'TryA_G Cuts at: 826 1045 2027 3265 4400 5081 5272 826 Size: 219 982 1238 1135 681 191 1471 Fragments arranged by size: 1471 1238 1135 982 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2310 4021 4922 5184 820 Size: 197 926 367 1711 901 262 1553 Fragments arranged by size: 1711 1553 926 901 367 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2004 3356 513 Size: 552 939 1352 3074 Fragments arranged by size: 3074 1352 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4163 5875 96 Size: 274 80 33 51 1443 2186 1712 138 Fragments arranged by size: 2186 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 2135 2422 1316 Size: 819 287 4811 Fragments arranged by size: 4811 819 287 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 2310 1943 Size: 367 5550 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 2439 2645 4046 2439 Size: 206 1401 4310 Fragments arranged by size: 4310 1401 206 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3010 3725 276 Size: 53 83 186 936 1476 715 2468 Fragments arranged by size: 2468 1476 936 715 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI