Restriction map for
pFN21AB8156
(Linear) (Six-base) MAPSORT of: KIBB8156.seq Check: 5373 from: 1 to: 1212
KIBB8156.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 1212
Size: 282 930
AatII G_ACGT'C
Cuts at: 0 1007 1212
Size: 1007 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 424 1212
Size: 76 348 788
Fragments arranged by size:
788 348 76
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 1212
Size: 1156 32 24
Fragments arranged by size:
1156 32 24
AlwNI CAG_nnn'CTG
Cuts at: 0 324 1212
Size: 324 888
ApaLI G'TGCA_C
Cuts at: 0 145 1212
Size: 145 1067
ApoI r'AATT_y
Cuts at: 0 136 358 1045 1086 1212
Size: 136 222 687 41 126
Fragments arranged by size:
687 222 136 126 41
AvaI C'yCGr_G
Cuts at: 0 13 305 1212
Size: 13 292 907
Fragments arranged by size:
907 292 13
BanII G_rGCy'C
Cuts at: 0 181 776 881 1212
Size: 181 595 105 331
Fragments arranged by size:
595 331 181 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 418 1212
Size: 215 203 794
Fragments arranged by size:
794 215 203
BbvCI CC'TCA_GC
Cuts at: 0 1016 1212
Size: 1016 196
BclI T'GATC_A
Cuts at: 0 504 1212
Size: 504 708
BglII A'GATC_T
Cuts at: 0 55 703 1212
Size: 55 648 509
Fragments arranged by size:
648 509 55
BlpI GC'TnA_GC
Cuts at: 0 809 1212
Size: 809 403
Bme1580I G_kGCm'C
Cuts at: 0 149 1212
Size: 149 1063
BmrI ACTGGGnnnn_n'
Cuts at: 0 334 1212
Size: 334 878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1212
Size: 620 592
Bpu10I CC'TnA_GC
Cuts at: 0 914 1016 1212
Size: 914 102 196
Fragments arranged by size:
914 196 102
BsaBI GATnn'nnATC
Cuts at: 0 60 509 1212
Size: 60 449 703
Fragments arranged by size:
703 449 60
BsaHI Gr'CG_yC
Cuts at: 0 1004 1212
Size: 1004 208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1156 1186 1212
Size: 1156 30 26
Fragments arranged by size:
1156 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 871 1212
Size: 49 822 341
Fragments arranged by size:
822 341 49
BseYI C'CCAG_C
Cuts at: 0 256 1212
Size: 256 956
BsiHKAI G_wGCw'C
Cuts at: 0 149 881 1212
Size: 149 732 331
Fragments arranged by size:
732 331 149
Bsp1286I G_dGCh'C
Cuts at: 0 149 181 776 881 1212
Size: 149 32 595 105 331
Fragments arranged by size:
595 331 149 105 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 1212
Size: 282 930
BstYI r'GATC_y
Cuts at: 0 55 510 703 918 1212
Size: 55 455 193 215 294
Fragments arranged by size:
455 294 215 193 55
BtgI C'CryG_G
Cuts at: 0 5 1212
Size: 5 1207
BtsI GCAGTG_nn'
Cuts at: 0 160 1212
Size: 160 1052
EaeI y'GGCC_r
Cuts at: 0 2 1212
Size: 2 1210
EarI CTCTTCn'nnn_
Cuts at: 0 764 1093 1212
Size: 764 329 119
Fragments arranged by size:
764 329 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 540 1212
Size: 540 672
EcoICRI GAG'CTC
Cuts at: 0 879 1212
Size: 879 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 424 620 1212
Size: 76 348 196 592
Fragments arranged by size:
592 348 196 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 1212
Size: 799 413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 454 939 1212
Size: 309 145 485 273
Fragments arranged by size:
485 309 273 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 690 722 1212
Size: 116 32 542 32 490
Fragments arranged by size:
542 490 116 32 32
MslI CAynn'nnrTG
Cuts at: 0 713 1212
Size: 713 499
MspA1I CmG'CkG
Cuts at: 0 7 444 1212
Size: 7 437 768
Fragments arranged by size:
768 437 7
NspI r_CATG'y
Cuts at: 0 897 1159 1212
Size: 897 262 53
Fragments arranged by size:
897 262 53
PfoI T'CCnGG_A
Cuts at: 0 494 1212
Size: 494 718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 1212
Size: 1156 32 24
Fragments arranged by size:
1156 32 24
PstI C_TGCA'G
Cuts at: 0 26 1212
Size: 26 1186
SacI G_AGCT'C
Cuts at: 0 881 1212
Size: 881 331
SacII CC_GC'GG
Cuts at: 0 8 1212
Size: 8 1204
SapI GCTCTTCn'nnn_
Cuts at: 0 764 1212
Size: 764 448
SfcI C'TryA_G
Cuts at: 0 22 1212
Size: 22 1190
SmlI C'TyrA_G
Cuts at: 0 305 1212
Size: 305 907
Tth111I GACn'n_nGTC
Cuts at: 0 130 417 1212
Size: 130 287 795
Fragments arranged by size:
795 287 130
XhoI C'TCGA_G
Cuts at: 0 305 1212
Size: 305 907
XmnI GAAnn'nnTTC
Cuts at: 0 434 640 1212
Size: 434 206 572
Fragments arranged by size:
572 434 206
ZraI GAC'GTC
Cuts at: 0 1005 1212
Size: 1005 207
Enzymes that do cut:
AarI AatII AcuI AloI AlwNI ApaLI ApoI AvaI
BanII BbsI BbvCI BclI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BstYI BtgI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI FalI Hin4I MslI MspA1I NspI
PfoI PpiI PstI SacI SacII SapI SfcI SmlI
Tth111I XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BfrBI BglI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI XbaI XcmI XmaI