(Circular) (Six-base) MAPSORT of: pFN21AB8154.seq Check: 111 from: 1 to: 6412 pFN21AB8154 6412 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4222 278 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3755 1937 Size: 1818 4594 Acc65I G'GTAC_C Cuts at: 3733 3733 Size: 6412 AclI AA'CG_TT Cuts at: 1563 2747 4539 4912 1563 Size: 1184 1792 373 3063 Fragments arranged by size: 3063 1792 1184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2818 2902 3154 3238 4475 5859 6127 1632 Size: 1186 84 252 84 1237 1384 268 1917 Fragments arranged by size: 1917 1384 1237 1186 268 252 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6215 AflIII A'CryG_T Cuts at: 1203 5311 6288 1203 Size: 4108 977 1327 Fragments arranged by size: 4108 1327 977 AgeI A'CCGG_T Cuts at: 5243 5243 Size: 6412 AhdI GACnn_n'nnGTC Cuts at: 5141 5141 Size: 6412 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6412 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6412 AlwNI CAG_nnn'CTG Cuts at: 4155 5727 4155 Size: 1572 4840 ApaLI G'TGCA_C Cuts at: 3600 4469 5625 3600 Size: 869 1156 4387 Fragments arranged by size: 4387 1156 869 ApoI r'AATT_y Cuts at: 1493 2044 2089 2264 2700 3036 3120 3456 3540 Size: 551 45 175 436 336 84 336 84 Cuts at: 3540 3624 3721 3947 4099 6208 1493 Size: 84 97 226 152 2109 1697 Fragments arranged by size: 2109 1697 551 436 336 336 226 175 152 97 84 84 84 45 AseI AT'TA_AT Cuts at: 160 1032 2429 4966 160 Size: 872 1397 2537 1606 Fragments arranged by size: 2537 1606 1397 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 AvaI C'yCGr_G Cuts at: 1870 1943 3737 1870 Size: 73 1794 4545 Fragments arranged by size: 4545 1794 73 BamHI G'GATC_C Cuts at: 3742 4109 3742 Size: 367 6045 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3733 5188 618 Size: 325 217 627 1946 1455 1842 Fragments arranged by size: 1946 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2196 2678 3731 1391 Size: 805 482 1053 4072 Fragments arranged by size: 4072 1053 805 482 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2370 2656 928 Size: 971 471 286 4684 Fragments arranged by size: 4684 971 471 286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4603 4637 1903 Size: 34 2666 34 3678 Fragments arranged by size: 3678 2666 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2500 3092 4304 5514 2500 Size: 592 1212 1210 3398 Fragments arranged by size: 3398 1212 1210 592 BclI T'GATC_A Cuts at: 1548 3274 1548 Size: 1726 4686 BfrBI ATG'CAT Cuts at: 2726 2726 Size: 6412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5023 136 Size: 107 122 71 631 605 3351 1525 Fragments arranged by size: 3351 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6407 6407 Size: 6412 BlpI GC'TnA_GC Cuts at: 3024 3829 3024 Size: 805 5607 Bme1580I G_kGCm'C Cuts at: 1790 3604 4473 5629 1790 Size: 1814 869 1156 2573 Fragments arranged by size: 2573 1814 1156 869 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6412 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2346 4143 5101 Size: 416 53 83 399 24 929 1797 958 Cuts at: 5101 442 Size: 1753 Fragments arranged by size: 1797 1753 958 929 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6412 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2730 2814 2898 2982 3150 3234 3318 3402 Size: 1491 84 84 84 168 84 84 84 Cuts at: 3402 3486 3570 5072 1239 Size: 84 84 1502 2579 Fragments arranged by size: 2579 1502 1491 168 84 84 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2037 4537 5402 5700 2037 Size: 2500 865 298 2749 Fragments arranged by size: 2749 2500 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2962 3130 5075 882 Size: 617 1463 168 1945 2219 Fragments arranged by size: 2219 1945 1463 617 168 BsaAI yAC'GTr Cuts at: 493 1204 4203 493 Size: 711 2999 2702 Fragments arranged by size: 2999 2702 711 BsaBI GATnn'nnATC Cuts at: 3279 6406 3279 Size: 3127 3285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4219 4601 5236 275 Size: 53 83 186 936 2686 382 635 1451 Fragments arranged by size: 2686 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4845 5243 5517 5664 6075 4845 Size: 398 274 147 411 5182 Fragments arranged by size: 5182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2133 2163 1178 Size: 30 165 30 730 30 5427 Fragments arranged by size: 5427 730 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6412 BseYI C'CCAG_C Cuts at: 2398 5615 6111 2398 Size: 3217 496 2699 Fragments arranged by size: 3217 2699 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5839 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3862 4623 5651 1540 Size: 275 186 1861 761 1028 2301 Fragments arranged by size: 2301 1861 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2678 3604 4473 4558 5629 2678 Size: 926 869 85 1071 3461 Fragments arranged by size: 3461 1071 926 869 85 BsmI GAATG_Cn' Cuts at: 2848 3436 3923 4016 2848 Size: 588 487 93 5244 Fragments arranged by size: 5244 588 487 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2678 3604 3731 4473 4558 5629 Size: 399 406 482 926 127 742 85 1071 Cuts at: 5629 1391 Size: 2174 Fragments arranged by size: 2174 1071 926 742 482 406 399 127 85 BspEI T'CCGG_A Cuts at: 6075 6075 Size: 6412 BspHI T'CATG_A Cuts at: 3616 4301 6031 3616 Size: 685 1730 3997 Fragments arranged by size: 3997 1730 685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3767 844 Size: 2923 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4299 1394 Size: 429 2476 3507 Fragments arranged by size: 3507 2476 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4907 5081 65 Size: 1183 3659 174 1396 Fragments arranged by size: 3659 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5056 5243 1700 Size: 110 3246 187 2869 Fragments arranged by size: 3246 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2094 3109 96 Size: 1998 1015 3399 Fragments arranged by size: 3399 1998 1015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6412 BssSI C'ACGA_G Cuts at: 4472 5484 4472 Size: 1012 5400 BstAPI GCAn_nnn'nTGC Cuts at: 794 3188 3440 3608 794 Size: 2394 252 168 3598 Fragments arranged by size: 3598 2394 252 168 BstBI TT'CG_AA Cuts at: 2976 5216 2976 Size: 2240 4172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6412 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 6412 BstYI r'GATC_y Cuts at: 1972 3518 3742 4109 4494 4511 5963 6049 6061 Size: 1546 224 367 385 17 1452 86 12 Cuts at: 6061 6407 1972 Size: 346 1977 Fragments arranged by size: 1977 1546 1452 385 367 346 224 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5860 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5673 Fragments arranged by size: 5673 683 56 BtsI GCAGTG_nn' Cuts at: 3928 4722 4742 3928 Size: 794 20 5598 Fragments arranged by size: 5598 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6377 DraI TTT'AAA Cuts at: 2254 3716 2254 Size: 1462 4950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5419 809 Size: 507 4103 1802 Fragments arranged by size: 4103 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3859 4748 6377 Size: 54 1425 50 275 18 2029 889 1629 Cuts at: 6377 8 Size: 43 Fragments arranged by size: 2029 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3859 1537 Size: 275 2047 4090 Fragments arranged by size: 4090 2047 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2694 4342 1353 Size: 1341 1648 3423 Fragments arranged by size: 3423 1648 1341 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4999 5373 5519 387 Size: 1064 138 3410 374 146 1280 Fragments arranged by size: 3410 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2676 2676 Size: 6412 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2730 2814 2818 2898 2902 2982 3150 Size: 393 1098 84 4 80 4 80 168 Cuts at: 3150 3154 3234 3238 3318 3402 3486 3570 4475 Size: 4 80 4 80 84 84 84 905 Cuts at: 4475 5072 5859 6127 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1098 905 787 597 393 268 168 84 84 84 84 80 80 80 80 4 4 4 4 EcoRI G'AATT_C Cuts at: 2700 3036 3120 3456 3540 3624 3721 4099 2700 Size: 336 84 336 84 84 97 378 5013 Fragments arranged by size: 5013 378 336 336 97 84 84 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2468 2468 Size: 6412 FspI TGC'GCA Cuts at: 4918 4918 Size: 6412 HaeII r_GCGC'y Cuts at: 1678 5559 1678 Size: 3881 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 2929 2961 5053 Size: 32 135 32 581 32 744 32 2092 Cuts at: 5053 5085 5127 5159 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2092 744 581 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3756 4002 669 Size: 988 281 1818 246 3079 Fragments arranged by size: 3079 1818 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6412 HpaI GTT'AAC Cuts at: 4002 4002 Size: 6412 KpnI G_GTAC'C Cuts at: 3737 3737 Size: 6412 MfeI C'AATT_G Cuts at: 4011 4011 Size: 6412 MluI A'CGCG_T Cuts at: 6288 6288 Size: 6412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5526 5710 1305 Size: 786 3435 184 2007 Fragments arranged by size: 3435 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4590 Fragments arranged by size: 4590 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 4370 4729 4888 518 Size: 648 1393 1811 359 159 2042 Fragments arranged by size: 2042 1811 1393 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2398 3828 4505 5653 5898 1606 Size: 792 1430 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 792 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5860 NdeI CA'TA_TG Cuts at: 387 2287 387 Size: 1900 4512 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6412 NotI GC'GGCC_GC Cuts at: 3859 3859 Size: 6412 NsiI A_TGCA'T Cuts at: 2728 2728 Size: 6412 NspI r_CATG'y Cuts at: 1132 1229 2730 3770 5315 1132 Size: 97 1501 1040 1545 2229 Fragments arranged by size: 2229 1545 1501 1040 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6412 PciI A'CATG_T Cuts at: 5311 5311 Size: 6412 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6412 PmeI GTTT'AAAC Cuts at: 3716 3716 Size: 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4481 4513 5994 6026 4481 Size: 32 1481 32 4867 Fragments arranged by size: 4867 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6412 PsiI TTA'TAA Cuts at: 3982 3982 Size: 6412 PstI C_TGCA'G Cuts at: 830 3764 830 Size: 2934 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6412 PvuII CAG'CTG Cuts at: 2398 2398 Size: 6412 SacI G_AGCT'C Cuts at: 2678 2678 Size: 6412 SalI G'TCGA_C Cuts at: 1936 3754 1936 Size: 1818 4594 SbfI CC_TGCA'GG Cuts at: 3764 3764 Size: 6412 ScaI AGT'ACT Cuts at: 4660 6372 4660 Size: 1712 4700 SfcI C'TryA_G Cuts at: 826 1045 2119 3511 3696 3760 4895 5576 5767 Size: 219 1074 1392 185 64 1135 681 191 Cuts at: 5767 826 Size: 1471 Fragments arranged by size: 1471 1392 1135 1074 681 219 191 185 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6412 SmaI CCC'GGG Cuts at: 1872 3739 1872 Size: 1867 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2052 4516 5417 5679 820 Size: 197 926 109 2464 901 262 1553 Fragments arranged by size: 2464 1553 926 901 262 197 109 SnaBI TAC'GTA Cuts at: 493 4203 493 Size: 3710 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6412 SphI G_CATG'C Cuts at: 1132 3770 1132 Size: 2638 3774 SspI AAT'ATT Cuts at: 5 52 4336 5 Size: 47 4284 2081 Fragments arranged by size: 4284 2081 47 StuI AGG'CCT Cuts at: 1517 2999 3335 3438 1517 Size: 1482 336 103 4491 Fragments arranged by size: 4491 1482 336 103 StyI C'CwwG_G Cuts at: 513 1065 2010 3851 513 Size: 552 945 1841 3074 Fragments arranged by size: 3074 1841 945 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4439 4456 4609 4794 5239 325 Size: 1255 2859 17 153 185 445 1498 Fragments arranged by size: 2859 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 3109 4658 Size: 274 80 33 51 1443 117 1015 1549 Cuts at: 4658 6370 96 Size: 1712 138 Fragments arranged by size: 1712 1549 1443 1015 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6372 6404 6372 Size: 32 6380 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6412 XbaI T'CTAG_A Cuts at: 3748 3748 Size: 6412 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2235 2235 Size: 6412 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6412 XmaI C'CCGG_G Cuts at: 1870 3737 1870 Size: 1867 4545 XmnI GAAnn'nnTTC Cuts at: 2451 2663 2747 2831 3167 3251 3587 4541 2451 Size: 212 84 84 336 84 336 954 4322 Fragments arranged by size: 4322 954 336 336 212 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4220 276 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI